Electrochemical sensing of NADH on NiO nanoparticles-modified carbon paste electrode and fabrication of ethanol dehydrogenase-based biosensor

Aydoğdu, Gözde; Zeybek, Derya Koyuncu; Zeybek, Bülent; Pekyardımcı, Şule

doi:10.1007/s10800-013-0536-3

Cited by 50 publications

(12 citation statements)

References 48 publications

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“…For examples, alumina/graphene oxide nanocomposite modified electrode showed NADH oxidation at 0.45 V and the linear range of detection was from 30–330 μM [12] . Nickel oxide nanoparticles modified carbon paste electrode was showed the NADH oxidation at 0.4 V and linear range of detection was from 0.1 to 1.0 mM [55] . The graphite nanosheet modified GCE showed NADH oxidation at 0.32 V and the linear range was from 2 μM to 4.69 mM [56] .…”

Section: Resultsmentioning

confidence: 99%

“…[12] Nickel oxide nanoparticles modified carbon paste electrode was showed the NADH oxidation at 0.4 V and linear range of detection was from 0.1 to 1.0 mM. [55] The graphite nanosheet modified GCE showed NADH oxidation at 0.32 V and the linear range was from 2 μM to 4.69 mM. [56] So, it was obvious that GrÀ W/GCE had reduced the NADH overpotential compared to other reported methods.…”

Section: Electro-catalytic Oxidation Of Nadhmentioning

confidence: 96%

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Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode

2020

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Reduced form of β‐nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD+) are the main cofactors involved in more than 300 dehydrogenase reactions. NAD+ is one of the important oxidizing agent for the oxidation of alcohol, aldehyde and ketones. Similarly, NADH has been used for the treatment of Alzheimer and Parkinson's diseases. Herein, we have reported synthesis of graphene flakes by electrochemical exfoliation of graphite in sodium tungstate solution. It was found that tungstate (WO42−) and hydroxyl (OH−) ions were intercalated into graphite layers and enabled the production of graphene flakes. As‐obtained graphene flakes were characterized by UV‐Visible (UV‐Vis), Fourier transform infrared (FT‐IR), Raman, Field emission scanning electron microscopy (FE‐SEM), Energy dispersive X‐ray (EDX) and High‐resolution transmission electron microscopies (HR‐TEM). FT‐IR, Raman and EDX analysis were confirmed that tungstate (WO42−) ions were present on the surface of graphene flakes. Moreover, graphene‐WO42− (Gr−W) dispersion was prepared by probe‐sonication to form a thin‐film on the surface of glassy carbon electrode (Gr−W/GCE). Interestingly, Gr‐W modified GCE reduced the overpotentials of NADH oxidation and NAD+ reduction. This new senor was also showed linear responses for NADH and NAD+ from 10–270 μM and 100–500 μM, respectively. Furthermore, the selectivity of the Gr−W/GCE was tested in the presence of L‐tyrosine, L‐isoleucine, L‐alanine, glutathione, dopamine (DA), ascorbic acid (AA), uric acid (UA), oxalic acid (OA), glucose, hydrogen peroxide (H2O2), acetaminophen (PA), potassium chloride (KCl) and sodium chloride (NaCl). It was found that selective detection of both NADH and NAD+ could be achieved by using Gr−W/GCE. Finally, the real application of the sensor was demonstrated by accurately detecting spiked NADH concentrations in human blood serum. The recovery analysis was also confirmed that Gr−W/GCE could be useful to detect NADH in biological samples.

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Section: Resultsmentioning

confidence: 99%

Section: Electro-catalytic Oxidation Of Nadhmentioning

confidence: 96%

Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode

2020

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“…The GC/CVD‐MWCNT/PT electrode (Figure c) displays little difference with or without PT modification (increase of only 9±3 %at 1 mM NADH), presumably due to the presence of a large amount of metal impurities, which have been shown to oxidise NADH catalytically . In this case, the NADH electrocatalysis may be dominated by the metallic impurities with a negligible contribution from PT.…”

Section: Resultsmentioning

confidence: 99%

Variation of Carbon Based Materials on the Electropolymerization of Tyramine

et al. 2018

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The electropolymerization of tyramine has been investigated using glassy carbon electrodes modified with six classes of carbon materials, namely carbon black, graphitized carbon, graphite, graphene oxide, chemical vapour deposition based multi‐walled carbon nanotubes and arc discharged based multi‐walled carbon nanotubes. These materials were characterized before and after electrodeposition of polytyramine (PT) by Raman spectroscopy, inductively coupled plasma optical emission spectrometry, cyclic voltammetry and amperometry. Previously, other groups have established that impurities present in carbon materials, can play a critical role in electrocatalytic activity. In this study, the presence of graphitic basal plane carbon, rather than metallic impurities, is believed to initiate the formation of a redox active PT film that mediates the oxidation of NADH. The importance of graphitic basal plane carbon was supported by examining the impact of the graphitization of carbon black over the range of 500 to 2000 °C. Graphitic basal plane carbon impurity was demonstrated to be highly active and important with respect to the electrodeposition of a PT film that possesses significant catalytic activity towards the oxidation of NADH.

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“…The relationship (Figure 4 inset) between the current and the concentration of NADH is displayed linear concentration ranges from 1.0 to 500.0 μM, sensitivity of 18.9 μA mM À 1 cm À 2 (R 2 = 0.9989), and a low limit of detection (LOD) of 0.002 μM was obtained (S/N = 3). The comparison of amperometric responses of different electrodes with previously reported NADH sensing systems [2,[37][38][39][40][41][42] has been presented in Table 1. The NiNPs/ERGO-ITO electrode prepared in this study was observed to exhibit both lower amperometric response and higher sensitivity to NADH in comparison with others.…”

Section: Amperometric Detection Of Nadh On Ninps/ergo-itomentioning

confidence: 99%

One‐pot Electrochemical Synthesis of Ni Nanoparticles‐decorated Electroreduced Graphene Oxide for Improved NADH Sensing

et al. 2020

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Ni nanoparticles (NPs) decorated electrochemical reduced graphene oxide (NiNPs/ERGO) nanocomposites (NCs) on ITO electrode were successfully fabricated using one-pot simultaneously electrochemical co-reduction method at room temperature. The NiNPs/ERGO electrodes demonstrated very good electrocatalytic activity in the oxidation of NADH at a low oxidation potential (~+ 430 mV), a wide linear range (1-500 μM), a calculated detection limit of 0.002 μM. Compared to ERGO and NiNPs electrodes, the synthesized NiNPs/ERGO NCs have high electrocatalytic activity due to synergistic effect emerged between NiNPs and ERGO. The results indicated that the NiNPs/ERGO NCs can be easily prepared by one-pot electrochemical technique and NiNPs/ERGO could used to as potential biosensor for the NADH detection.

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Electrochemical sensing of NADH on NiO nanoparticles-modified carbon paste electrode and fabrication of ethanol dehydrogenase-based biosensor

Cited by 50 publications

References 48 publications

Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode

Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode

Variation of Carbon Based Materials on the Electropolymerization of Tyramine

One‐pot Electrochemical Synthesis of Ni Nanoparticles‐decorated Electroreduced Graphene Oxide for Improved NADH Sensing

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Electrochemical sensing of NADH on NiO nanoparticles-modified carbon paste electrode and fabrication of ethanol dehydrogenase-based biosensor

Cited by 50 publications

References 48 publications

Selective Electrochemical Sensing of NADH and NAD+ Using Graphene/Tungstate Nanocomposite Modified Electrode

Selective Electrochemical Sensing of NADH and NAD+ Using Graphene/Tungstate Nanocomposite Modified Electrode

Variation of Carbon Based Materials on the Electropolymerization of Tyramine

One‐pot Electrochemical Synthesis of Ni Nanoparticles‐decorated Electroreduced Graphene Oxide for Improved NADH Sensing

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Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode

Selective Electrochemical Sensing of NADH and NAD⁺ Using Graphene/Tungstate Nanocomposite Modified Electrode