Electrochemical sensor for enzymatic lactate detection based on laser-scribed graphitic carbon modified with platinum, chitosan and lactate oxidase

Madden, Julia; Vaughan, Eoghan; Thompson, Michael; Riordan, Alan O’; Galvin, Paul; Iacopino, Daniela; Teixeira, Sofia Rodrigues

doi:10.1016/j.talanta.2022.123492

Cited by 28 publications

(19 citation statements)

References 33 publications

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“…Table 3 shows the comparisons of the characteristics of diverse LA biosensors. Madden et al showed slower electron transfer behavior due to chitosan and lactate oxidase modification, which could be attributed to the nonconductive chitosan covering an area of the Pt-LSG electrode [ 44 ]. Thus, it slowed down the diffusion of redox substances to the electrode.…”

Section: Resultsmentioning

confidence: 99%

The Characteristics Analysis of a Microfluid-Based EGFET Biosensor with On-Chip Sensing Film for Lactic Acid Detection

Kuo

Chang

Lai

et al. 2022

Sensors

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In this research, a microfluid-based extended gate field-effect transistor (EGFET) biosensor with an on-chip sensing window (OCSW) was fabricated. The detection window was composed of six metal layers, and a ruthenium dioxide (RuO2) film was spattered on the surface and functionalized with lactase to detect lactic acid (LA). To detect LA in a more diversified way, a microfluidic system was integrated with the biosensor. Moreover, a special package was used to seal the sensing window and microfluidic tube and insulate it from other parts to prevent water molecule invasion and chip damage. The sensitivity analysis of the EGFET biosensor was studied by a semiconductor parameter analyzer (SPA). The static and dynamic measurements of the EGFET with sensing windows on a chip were analyzed. The sensing characteristics of the EGFET biosensor were verified by the experimental results. The proposed biosensor is suitable for wearable applications due to the advantages of its low weight, low voltage, and simple manufacturing process.

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Section: Resultsmentioning

confidence: 99%

The Characteristics Analysis of a Microfluid-Based EGFET Biosensor with On-Chip Sensing Film for Lactic Acid Detection

Kuo

Chang

Lai

et al. 2022

Sensors

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“…Some researchers used the laser direct writing method to make a laser-scribed graphitic (LSG) carbon electrode on a polyimide (PI) substrate, then deposited Pt on the electrode, and finally poured chitosan (CS) and LOx on the electrode to prepare a flexible laser-engraved graphite carbon based lactate biosensors, which could detect lactate linearly in the range of 0.2–3 mM ( R 2 > 0.99 ) with the detection limit of 0.11 mM and the sensitivity of 35.8 µA mM −1 cm −2 , as shown in Figure 3 f [ 73 ]. Another research group fabricated the working electrode for a lactate sensor consisting of a mediating layer, which was made by drop-casting tetrathiafulvalene (TTF)/CNT suspension on the gold electrode, followed by LOx immobilized in the CS/CNT suspension.…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Recent Advances in Wearable Biosensors for Non-Invasive Detection of Human Lactate

Shen

Liu

et al. 2022

Biosensors

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Lactate, a crucial product of the anaerobic metabolism of carbohydrates in the human body, is of enormous significance in the diagnosis and treatment of diseases and scientific exercise management. The level of lactate in the bio-fluid is a crucial health indicator because it is related to diseases, such as hypoxia, metabolic disorders, renal failure, heart failure, and respiratory failure. For critically ill patients and those who need to regularly control lactate levels, it is vital to develop a non-invasive wearable sensor to detect lactate levels in matrices other than blood. Due to its high sensitivity, high selectivity, low detection limit, simplicity of use, and ability to identify target molecules in the presence of interfering chemicals, biosensing is a potential analytical approach for lactate detection that has received increasing attention. Various types of wearable lactate biosensors are reviewed in this paper, along with their preparation, key properties, and commonly used flexible substrate materials including polydimethylsiloxane (PDMS), polyethylene terephthalate (PET), paper, and textiles. Key performance indicators, including sensitivity, linear detection range, and detection limit, are also compared. The challenges for future development are also summarized, along with some recommendations for the future development of lactate biosensors.

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“…Moreover, chitosan has been explored for tissue engineering, sutures, and wound dressings, taking advantage of its bacteriostatic and healing properties [31][32][33][34]. Other applications include the development of biosensors [35].…”

Section: Chitosan-based Materials and Devicesmentioning

confidence: 99%

Applications of Chitosan in Surgical and Post-Surgical Materials

et al. 2022

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The continuous advances in surgical procedures require continuous research regarding materials with surgical applications. Biopolymers are widely studied since they usually provide a biocompatible, biodegradable, and non-toxic material. Among them, chitosan is a promising material for the development of formulations and devices with surgical applications due to its intrinsic bacteriostatic, fungistatic, hemostatic, and analgesic properties. A wide range of products has been manufactured with this polymer, including scaffolds, sponges, hydrogels, meshes, membranes, sutures, fibers, and nanoparticles. The growing interest of researchers in the use of chitosan-based materials for tissue regeneration is obvious due to extensive research in the application of chitosan for the regeneration of bone, nervous tissue, cartilage, and soft tissues. Chitosan can serve as a substance for the administration of cell-growth promoters, as well as a support for cellular growth. Another interesting application of chitosan is hemostasis control, with remarkable results in studies comparing the use of chitosan-based dressings with traditional cotton gauzes. In addition, chitosan-based or chitosan-coated surgical materials provide the formulation with antimicrobial activity that has been highly appreciated not only in dressings but also for surgical sutures or meshes.

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Electrochemical sensor for enzymatic lactate detection based on laser-scribed graphitic carbon modified with platinum, chitosan and lactate oxidase

Cited by 28 publications

References 33 publications

The Characteristics Analysis of a Microfluid-Based EGFET Biosensor with On-Chip Sensing Film for Lactic Acid Detection

The Characteristics Analysis of a Microfluid-Based EGFET Biosensor with On-Chip Sensing Film for Lactic Acid Detection

Recent Advances in Wearable Biosensors for Non-Invasive Detection of Human Lactate

Applications of Chitosan in Surgical and Post-Surgical Materials

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