Electrochemiluminescence detection of reduced and oxidized glutathione ratio by quantum dot-layered double hydroxide film

Abstract

The ratio of reduced and oxidized glutathione (GSH/GSSG ratio) is a greater first indication of disease risk than the total concentration of GSH. However, the interferences from thiolated biomolecules, especially cysteine (Cys), make the accurate detection of GSH/GSSG ratio a technical problem. In this work, we successfully used a mixture of quantum dots (QDs) and ZnAl-LDH nanosheets to fabricate a high electrochemiluminescence resonance energy transfer (ERET) efficiency sensor for GSH from the disturbances of amino acids, especially Cys and GSSG. The mechanisms of high ERET efficiency and selectivity were well investigated with spectroscopy analysis and theoretical calculation. The results showed that the interaction force between ZnAl-LDH nanosheets and molecules proved a long-range-ordered space and selective transmission for molecules. On the basis of these interesting phenomena, we successfully measured the GSH/GSSG ratio in whole blood and serum samples.

Sensitive and Selective Carmine Acid Detection Based on Chemiluminescence Quenching of Layer Doubled Hydroxide–Luminol–H2O2 System

“…24,25 With the integration of the electrochemical technique, sensitive peptide detection and enzyme activity evaluation are achieved. 26−28 The high sensitivity and the excellent characters encourage us to develop a simple CL approach for CA detection.…”

“…The sensitivity of H 2 O 2 detection using a luminol CL system is increased by 2 orders of magnitude with the assistance of LDH . By combining flow system and injection detector, sensitive CL detection systems have been established for dyes, thiols, H 2 O 2 , and so forth. , With the integration of the electrochemical technique, sensitive peptide detection and enzyme activity evaluation are achieved. − The high sensitivity and the excellent characters encourage us to develop a simple CL approach for CA detection.…”

Highly Stable Basswood Porous Carbon Anode Activated by Phosphoric Acid for a Sodium Ion Battery

“…More active sites are conducive to chemical reactions and electrode capacity increase. 31 In order to compare the carbon formation rate, Figure 2d shows the thermogravimetric analysis (TGA) of the four kinds of carbon material precursors under a N 2 atmosphere. Because of the addition of phosphoric acid in different ratios, the carbon-forming yields of the three kinds of phosphoric acidactivated carbons (PBC-21, PBC-11, and PBC-12) after rough calculation at 500 °C are about 51.5, 56.8, and 55.5%, much higher than 15.1% of BC.…”

“…This confirms that as the proportion of phosphoric acid increases, the defect points gradually increase and the degree of graphitization decreases (the electrical conductivity decreases). More active sites are conducive to chemical reactions and electrode capacity increase …”

Electroanalytical determination of reduced-glutathione in biological samples

“…In view of the important biological and clinical significance for GSH, it has thus spurred strong interest in developing useful chemical materials for quantitatively evaluating the level of intracellular GSH [4,8]. Glutathione is a tripeptide which can be synthesized in the liver using no genetic information [9][10][11][12]. It is a major antioxidant which protects cells from oxidative damage by reacting with free radicals and peroxides [7,9,10].…”