Electrochemiluminescence Resonance Energy Transfer System Based on Silver Metal–Organic Frameworks as a Double-Amplified Emitter for Sensitive Detection of miRNA-107

Abstract

As a class of electrochemiluminescence (ECL) enhancers, silver-based materials have broad application prospects. In this work, a novel silver metal–organic framework (AgMOF) was developed as a self-enhanced ECL emitter by one-step mixing and standing at room temperature. The AgMOF could produce strong and stable ECL emissions based on a double-amplification method, which originated from the aggregation-induced ECL emission of ligands and catalyzing S2O8 2– to produce more SO4 •– by silver. Moreover, an ECL resonance energy transfer (ECL-RET) biosensor with AgMOF as a donor and BHQ2 as an acceptor was fabricated by duplex-specific nuclease (DSN)-assisted target recycling amplification to detect miRNA-107. The biosensor exhibited a strong ECL-RET effect due to the higher ECL emission of the AgMOF and perfect match of spectra between the AgMOF and BHQ2. Upon the introduction of DSN and target miRNAs, the specific DNA–RNA binding and nuclease cleaving could trigger the detachment of BHQ2, resulting in an increased ECL signal of AgMOF. Benefiting from the ECL-RET and DSN-assisted target recycling amplification methods, this biosensor achieved a wide linear relationship range from 20 to 120 fM with a low limit of detection (4.33 fM). This research presents an effective emitter for self-enhanced ECL systems, which broadens the potential ECL applications of silver-based nanomaterials.

Fast Room‐Temperature Synthesis of an Extremely Alkaline‐Resistant Cationic Metal–Organic Framework for Sequestering TcO4− with Exceptional Selectivity

“…Though some cationic MOF materials could be obtained at room temperature, it still retained the defect of long reaction time. [50,51] In this work, the crystal of Ag-TPPE was first prepared hydrothermally by reacting 1,1,2,2-tetrakis(4-pyridylphenyl)ethylene (TPPE) (Figure S1, Supporting Information) with AgNO 3 in mixed solvent of CH 3 CN/DMSO (v/v, 10/4) at 100 °C for 48 h (Figure S2a, Supporting Information). Single-crystal X-ray diffraction indicated that Ag-TPPE remained identical four-interpenetrated networks as coordination polymer obtained by diffusion method (Table S1, Supporting Information).…”

Nucleic acid isothermal amplification-based soft nanoarchitectonics as an emerging electrochemical biosensing platform

“…One type of ECL-RET system consists of a nanomaterial luminophore and an organic (or inorganic) reagent as a donor/ acceptor pair. 124,125 As shown in Fig. 4A, Yang's group designed an ECL-RET system based on graphitic carbon nitride nanosheets (GCNNs) and Ru( phen) 3 2+ as a donor/ acceptor pairs, in which the signal was regulated by the amount Ru( phen) 3 2+ present.…”

Boron Carbon Nitride Nanosheets-Ru Nanocomposite Self-Enhancement Electrochemiluminescence Emitter with a Three-Dimensional DNA Network Structure as a Signal Amplifier for Ultrasensitive Detection of TK1 mRNA

“…Electrochemiluminescence (ECL), a form of energy relaxation, was triggered by the electrochemical stimulation, which was attracting increasing interest in a variety of areas, − such as clinical diagnosis, environmental regulation, food analysis, and drug detection on account of low background, high sensitivity, and excellent controllability. − In the coreactant ECL system, the coreactant was indispensable to improve the ECL efficiency of the emitter. − However, the reaction between the external coreactant and emitter in the traditional coreactant ECL system had certain disadvantages of slow electron transfer speed and low ECL efficiency, limiting the improvement of ECL strength. , Therefore, it was particularly important to explore an efficient ECL emitter to avoid the electron transmission obstruction and large energy loss between the emitter and coreactant in solution. On account of the π–π stacking and electrostatic interactions as well as the stimulation of electrical activity by heteropolar B–N bonding, boron carbon nitride nanosheets (BCN NSs) exhibited striking performance such as extraordinary chemical stability, excellent adsorption, and impressive oxygen reduction reaction catalysis. − Moreover, the electronic tunneling among the ultrathin BCN NSs’ interlamination could realize faster electron transfer, which was conducive to the rapid and effective intramolecular reaction.…”