Electrochemiluminescent biosensing of carbohydrate-functionalized CdS nanocomposites for in situ label-free analysis of cell surface carbohydrate

Recent advances in nanostructures and nanocrystals as signal-amplification elements in electrochemical cytosensing

“…Interestingly, an easy ECL strategy for in situ label-free monitoring of carbohydrate expression on living cells was designed by integrating the specific recognition of lectin to carbohydrate with a carbohydrate-functionalized CdS nanocomposite [31]. In this report, the mercaptopropionic acid-capped CdS-QD were first immobilized on a CNT-modified electrode and then functionalized with carbohydrate using mannan as a model on the surface (Scheme 6).…”

Quantum dot-based photoelectric conversion for biosensing applications

“…The MPA-capped CdS-QDs were also modified on carbon nanotubes (CNTs), then further modification with carbohydrate or capture antibody, or carbohydrate or antibody-modified CdS nanocomposite was successfully prepared with good ECL performance for cytosensing or immunoassay [37,38].…”

Sensitive biosensing strategy based on functional nanomaterials

“…The decrease in fluorescence intensity of the nanoprobe solution is related to the cell amount and the expression extent of the mannosyl groups on the K562 cells. A facile ECL strategy for label-free monitoring of carbohydrate expression on living cells has been designed based on carbohydrate-functionalized CdS QDs/CNTs nanocomposites, which act as ECL emitting species on sensor surface [107]. Upon competitive binding of Con A to the carbohydrate groups on both cell surface and electrode surface, the ECL changed, which depends on both the cell amount and the expression level of cell surface carbohydrate.…”