Electrochemistry and photoelectrochemistry of photosystem I adsorbed on hydroxyl-terminated monolayers

Ciobanu, Mădălina; Kincaid, Helen; Lo, Vivian; Dukes, Albert D.; Jennings, G. Kane; Cliffel, David E.

doi:10.1016/j.jelechem.2006.09.019

Cited by 76 publications

(92 citation statements)

References 22 publications

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“…On hydroxyl-terminated monolayers, 70 % of the immobilized proteins are oriented with the primary electron donor (P700 cofactor) close to the electrode. Clear reversible peaks for P700 were observed in the cyclic voltammetry of PSI from spinach leaves adsorbed on 6-mercapto-1-hexanol modified gold electrodes in the absence of light (Ciobanu et al , 2007 ). The signals of the terminal electron acceptors (the iron-sulfur clusters F A and F B ) further away from the electrode surface were detected by using the more sensitive square wave voltammetry technique only.…”

Section: Photosystemsmentioning

confidence: 99%

Recent advances in the electrochemistry and spectroelectrochemistry of membrane proteins

Melin

Hellwig

2013

Biological Chemistry

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Abstract:Integral membrane proteins are encountered in fundamental natural processes, such as photosynthesis and respiration. The relation between the structure of the proteins and their function and dynamics are still not clear in most cases. Once fully understood, these processes could ultimately help researchers to develop alternative methods for producing energy, either from light or biomass. They could also lead to more efficient antibiotics, which would selectively inhibit a specific membrane protein of pathogenic bacteria. Since the chemical reactions involved in both photosynthesis and respiration are redox reactions, electrochemical methods can play a considerable role in uncovering their mechanisms. The electrochemical characterization of membrane proteins is, however, quite challenging. An overview on the techniques used for the characterization of membrane proteins, including classical approaches such as voltammetry and spectroelectrochemistry, and recent developments, such as their combination with surface-enhanced techniques is given.

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Section: Photosystemsmentioning

confidence: 99%

Recent advances in the electrochemistry and spectroelectrochemistry of membrane proteins

Melin

Hellwig

2013

Biological Chemistry

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“…The effect of the immobilization on the properties of redox proteins has been discussed, often with emphasis on cytochromes adsorbed onto modified gold electrodes [275][276][277] 202 Noncatalytic data was also reported for a laccase covalently attached to a monolayer of thiol self-assembled on a gold electrode, 77 for Photosystem-I solubilized in detergent and adsorbed on modified gold, 286 and for a number of enzymes embedded into alternate layers of charged polymers or layers of surfactants. 258 This occurred for a bacterial reaction center, 287 photosystems I 288 and II, 289 bovine milk xanthine oxidase, 290 Rhodobacter capsulatus xanthine dehydrogenase 183 and DMSO reductase, [291][292][293] For adsorbed enzymes, a preliminary diagnosis of health can come from noncatalytic electrochemical studies themselves, since the conformity of the peaks to ideal predictions (section 2.1) reports on the homogeneity of reduction potentials, and the values of the measured reduction potentials can be compared to those measured under potentiometric, equilibrium titrations.…”

Section: Observing Reasonable Noncatalytic Signalsmentioning

confidence: 99%

Direct Electrochemistry of Redox Enzymes as a Tool for Mechanistic Studies

2008

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“…[ 4 ] Several developments have enabled dramatic improvements in the photo-generated currents that can be produced by PSI-electrode based devices. [ 5 ] These improvements include novel PSI immobilization methods on gold electrodes, [6][7][8] embedding PSI within polymer deveined and macerated followed by centrifugation at 8,000 g to isolate the thylakoid membranes. [ 20 ] The resulting pellet was then resuspended using a buffer with a high surfactant concentration to break open the thylakoid membrane.…”

Section: Introductionmentioning

confidence: 99%

Integration of Photosystem I with Graphene Oxide for Photocurrent Enhancement

LeBlanc

Winter

Crosby

et al. 2014

Advanced Energy Materials

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matrixes, [ 5 ] the use of thick protein fi lms, [ 9 ] and the use of semiconductor electrode materials. [ 10,11 ] The integration of PSI with carbon nano-materials, however, has been limited. The work of Carmeli and co-workers demonstrated how PSI could be covalently attached to carbon nanotubes. [ 12 ] Additionally, our research group demonstrated how PSI could be interfaced as a monolayer on a graphene electrode. [ 13 ] The use of carbon nanostructures such as graphene and carbon nanotubes to develop nano-composite materials has become an area of great research interest due to the unique properties of these materials. [ 14 ] The time-consuming and challenging preparation of graphene, however, has resulted in the increased interest in graphene oxide (GO). [ 15 ] GO, the oxidized form of graphene, is easily generated through the oxidation and subsequent exfoliation of graphite. The oxygen functional groups enable GO to be dispersed in polar solvents (i.e., water) and further functionalized. However, because the conjugation of the system is disrupted, the conductivity of GO is fi ve orders of magnitude lower than graphite. [ 16 ] To regain conductivity, GO is commonly reduced to generate reduced graphene oxide (RGO). [ 17 ] Both GO and RGO have been incorporated with various materials to develop new functional composites. [ 18,19 ] The addition of GO and RGO is particularly attractive for PSIbased devises for a number of reasons. GO and RGO are both water-soluble, making the integration of these materials with PSI straightforward. Furthermore, the low cost and facile synthesis of GO and RGO make these materials ideal candidates for making inexpensive composite materials. The functional groups present on GO make possible the interaction with both the polar groups of PSI as well as the electrochemical mediator. However, the improved conductivity of RGO can facilitate electron transfer between the PSI complexes and the underlying electrode. Here we describe how incorporating either GO or RGO with PSI can improve the photoelectrochemical properties of p-doped silicon. Experimental SectionExtraction and Isolation of Photosystem I : Photosystem I complexes were isolated from commercially available baby spinach leaves as described previously. [ 11 ] Briefl y, the baby spinach was Photosystem I is a photoactive membrane protein used in nature to photoexcite electrons with nearly unit internal quantum effi ciency, sparking interest in using this biomaterial for solar energy conversion. Films of PSI deposited on p-doped silicon have previously demonstrated signifi cant photocurrents with an electrochemical mediator; however, improvement in electron transfer is needed. Here, it is investigated how PSI can be combined with graphene oxide (GO) or reduced graphene oxide (RGO) to generate composite fi lms capable of improved photoelectrochemical performance. It is found that both composite fi lms outperformed the PSI fi lm alone, and the PSI-GO composite fi lm is found to perform the best. The enhancement is attributed to the d...

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Electrochemistry and photoelectrochemistry of photosystem I adsorbed on hydroxyl-terminated monolayers

Cited by 76 publications

References 22 publications

Recent advances in the electrochemistry and spectroelectrochemistry of membrane proteins

Recent advances in the electrochemistry and spectroelectrochemistry of membrane proteins

Direct Electrochemistry of Redox Enzymes as a Tool for Mechanistic Studies

Integration of Photosystem I with Graphene Oxide for Photocurrent Enhancement

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