Electrochemistry of horseradish peroxidase entrapped in graphene and dsDNA composite modified carbon ionic liquid electrode

Graphene for Biosensor Applications

“…As seen in Table 11, smaller proteins lead to a lower LOD. The same strategy was applied for analytes such as nitromethane, 280 trichloroacetic acid, 290 and chlorite, 291 leading to similar results.…”

Electrochemical performance of myoglobin based on TiO2-doped carbon nanofiber decorated electrode and its applications in biosensing

“…Two linear regression equations were calculated as I pc (mA) ¼ 93.23v (V s À1 ) + 4.28 (R ¼ 0.9914) and I pa (mA) ¼ À63.90v (V s À1 ) À 1.60 (R ¼ 0.9921), respectively, indicating a characteristic surface-conned thin-layer electrochemical process. The average surface concentration of electroactive Mb (G*, mol cm À2 ) could be estimated from the charge integration of the reduction peak based on the Faraday equation: Q ¼ nFAG*, 32 where F is the Faraday constant, Q is the charge by integrating the reduction peak of Mb, and n and A represent the number of electrons transferred and the geometrical surface area of the electrode, respectively. The number of electroactive Mb molecules was estimated to be 4.69 Â 10 À10 mol cm À2 , accounting for 8.20% of the total Mb on the electrode surface (5.72 Â 10 À9 mol cm À2 ).…”

“…While the TCA concentration was more than 105.0 mmol L À1 , Fig. 4 Cyclic voltammograms of Nafion/Mb/TiO 2 @CNF/CILE in pH 4.0 PBS in the presence of (A) 0, 3,6,10,15,20,26,32,38,44,50,60,70,80,90, 105 mmol L À1 TCA (curves a to p, inset is the linear relationship of catalytic reduction peak currents vs. TCA concentration); (B) 0, 6,9,12,20,30,40,50,60, 70 mmol L À1 NaNO 2 (curves a to j, inset is the linear relationship of catalytic reduction peak currents vs. NaNO 2 concentration); (C) 0, 1, 2, 3, 5, 7, 10, 15, 20, 25, 30, 32 mmol L À1 H 2 O 2 (curves a to l, inset is the linear relationship of the catalytic reduction peak currents vs. H 2 O 2 concentration).…”

Direct Electrochemistry of Horseradish Peroxidase Immobilized in a Low Molecular Weight Gel

“…[17,18] In the present study, HRP is tried to be entrapped in a gel formed by DDPB in acetone/H 2 O and the gelation is exploited to immobilize HRP on glassy carbon electrode (GCE) to fabricate an HRP-gel modified GCE electrode (HRP-gel/GCE). [17,18] In the present study, HRP is tried to be entrapped in a gel formed by DDPB in acetone/H 2 O and the gelation is exploited to immobilize HRP on glassy carbon electrode (GCE) to fabricate an HRP-gel modified GCE electrode (HRP-gel/GCE).…”