Electrochemistry of the Interaction between Bioactive Drugs Daunorubicin and Dopamine and DNA at a Water/Oil Interface

Ribeiro, Danilo Monteiro; Pereira, Carlos M.; Silva, A. Fernando

doi:10.1016/j.electacta.2015.08.074

Cited by 20 publications

(14 citation statements)

References 60 publications

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“…∆ G is defined in eqn (4) as:

italicΔG = italicΔH - italicTΔS

where ∆ G is Gibbs free energy, ∆ H is enthalpy change, ∆ S is entropy change and T is the temperature. Signs of ∆ H and ∆ S can reflect the binding mechanism between a small molecule and DNA: hydrophobic forces are dominant when ∆ H > 0 and ∆ S > 0; van der Waals interaction and hydrogen bonds are the main interactions contributing to the binding when ∆ H < 0 and ∆ S < 0; and electrostatic interactions are dominant when ∆ H < 0 and ∆ S > 0 eqn (5):

ln K_{a} = - \frac{ΔH}{RT} + \frac{ΔS}{R}

where K a is the binding constant at the corresponding temperature T , and R is the gas constant. As listed in Table , negative values of ∆ G and ∆ H and positive values of ∆ S were obtained, and this finding indicated that the binding of cephalosporin and DNA is a spontaneous process driven by both enthalpy and entropy, and electrostatic interactions are the main binding force .…”

Section: Resultsmentioning

confidence: 99%

Comparative study of two cephalosporin antibiotics binding to calf thymus DNA by multispectroscopy, electrochemistry, and molecular docking

et al. 2019

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The over-use of antibiotics has caused a number of problems such as contamination of antibiotic residues and virus resistance, and therefore has attracted global attention. In this study, spectroscopic techniques and molecular docking were employed to predict conformational changes and binding interaction between two cephalosporins (cefaclor and cefixime) and calf thymus DNA (ctDNA). Fluorescence and UV-vis spectra suggested that static quenching was predominant and cephalosporin bound to the groove region of ctDNA. Binding parameters calculated by the Stern-Volmer and Scatchard equations showed that cephalosporin bound to ctDNA with a binding affinity in the order of 10 3 L mol −1 . Thermodynamic parameters further indicated that the reaction was a spontaneous process driven by enthalpy and entropy, and that the main binding force was an electrostatic force. The effects of iodide, denaturant, thermal denaturation and pH on a cephalosporin-Hoechst-DNA complex were also studied, and the results confirmed that cephalosporin bound to the groove area of DNA.Finally, these results were further confirmed by molecular docking and electrochemical studies.

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“…∆ G is defined in eqn (4) as:

italicΔG = italicΔH - italicTΔS

ln K_{a} = - \frac{ΔH}{RT} + \frac{ΔS}{R}

Section: Resultsmentioning

confidence: 99%

Comparative study of two cephalosporin antibiotics binding to calf thymus DNA by multispectroscopy, electrochemistry, and molecular docking

et al. 2019

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“…The usage of nucleic acid layers combined with electrochemical or optical transducers produce a new kind of affinity biosensors for the detection of molecular interactions between double stranded DNA (dsDNA) and the target pollutants or drugs for screening of these compounds [1][2][3][4]. As a result of these applications, there has been a growing interest in the usage of these biosensors in the electrochemical investigation of interactions between anticancer drugs and DNA [5][6][7][8][9][10][11][12][13][14] by using the advantages of electrochemical methods such as rapid, simple and low-cost point-of-care detection. For the electrochemical detection of the interactions between drug and DNA, the changes at oxidation peak current of the drug or the electroactive DNA bases were observed.…”

Section: Introductionmentioning

confidence: 99%

“…The interaction was studied in aqueous medium or on electrode surface by using glassy carbon electrode (GCE) in connection with using square-wave voltammetry (SWV) and differential pulse voltammetry (DPV). Ribeiro et al [12] described a voltammetric method based on the ion transfer at a water/oil interface for the electrochemical study of the interaction between high molecular weight dsDNA and two molecules of biological interest: the anthracycline drug daunorubicin (DNR) and the neurotransmitter dopamine (DA) using DPV technique. Shervedani et al [13] reported the immobilization of methotrexate (MTX) anticancer drug onto the graphene surface and its interactions with ctDNA and 4T1 cancer cells by using surface analysis techniques and electrochemical methods.…”

Section: Introductionmentioning

confidence: 99%

“…Ribeiro et al [12] described a voltammetric method based on the ion transfer at a water/oil interface for the electrochemical study of the interaction between high molecular weight dsDNA and two molecules of biological interest: the anthracycline drug daunorubicin (DNR) and the neurotransmitter dopamine (DA) using DPV technique. Shervedani et al [13] reported the immobilization of methotrexate (MTX) anticancer drug onto the graphene surface and its interactions with ctDNA and 4T1 cancer cells by using surface analysis techniques and electrochemical methods. Erdem et al [14] used the DNA-modified CPE in combination with CV and DPV to investigate the interaction of EPR with dsDNA and ssDNA and observed the changes in the EPR signals caused by the intercalation of EPR into dsDNA.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical detection of anticancer drug Lumazine and DNA interaction by using carbon nanotube modified electrodes

Karadeniz¹,

Ekşin²,

Erdem³

2019

jrp

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The interaction of lumazine (LMZ) with dsDNA was investigated with differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) by using disposable carbon nanotubes-modified pencil graphite electrode (CNTs-PGE). The passive adsorption process was used to modify the electrode surface by CNTs and then dsDNA was immobilized onto the modified PGEs. The performance of CNT-PGEs was studied by examining the optimum analytical conditions with DPV and then impedance method was performed to characterize the modification of CNTs onto the electrode surface.

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“…The principle of the electrochemical detection of nucleic acids is based on the detection of redox signals, mediated by electro-activity of bases (adenine, guanine and cytosine). Square wave voltammetry (SWV), cyclic voltammetry (CV), elimination voltammetry (EV) and differential pulse voltammetry (DPV) can be employed in the detection of nucleic acids [ 21 , 22 , 23 ]. SWV in combination with the adsorptive transfer stripping (AdTS) technique increased sensitivity and decrease sample consumption [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection

Jiménez

Ruttkay-Nedecký

Dostálová

et al. 2016

IJMS

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The majority of carcinomas that were developed due to the infection with human papillomavirus (HPV) are caused by high-risk HPV types, HPV16 and HPV18. These HPV types contain the E6 and E7 oncogenes, so the fast detection of these oncogenes is an important point to avoid the development of cancer. Many different HPV tests are available to detect the presence of HPV in biological samples. The aim of this study was to design a fast and low cost method for HPV identification employing magnetic isolation, polymerase chain reaction (PCR) and electrochemical detection. These assays were developed to detect the interactions between E6-HPV16 oncogene and magnetizable particles (MPs) using commercial Dynabeads M-280 Streptavidin particles and laboratory-synthesized “homemade” particles called MANs (MAN-37, MAN-127 and MAN-164). The yields of PCR amplification of E6-HPV16 oncogene bound on the particles and after the elution from the particles were compared. A highest yield of E6-HPV16 DNA isolation was obtained with both MPs particles commercial M-280 Streptavidin and MAN-37 due to reducing of the interferents compared with the standard PCR method. A biosensor employing the isolation of E6-HPV16 oncogene with MPs particles followed by its electrochemical detection can be a very effective technique for HPV identification, providing simple, sensitive and cost-effective analysis.

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Electrochemistry of the Interaction between Bioactive Drugs Daunorubicin and Dopamine and DNA at a Water/Oil Interface

Cited by 20 publications

References 60 publications

Comparative study of two cephalosporin antibiotics binding to calf thymus DNA by multispectroscopy, electrochemistry, and molecular docking

Comparative study of two cephalosporin antibiotics binding to calf thymus DNA by multispectroscopy, electrochemistry, and molecular docking

Electrochemical detection of anticancer drug Lumazine and DNA interaction by using carbon nanotube modified electrodes

Specific Magnetic Isolation of E6 HPV16 Modified Magnetizable Particles Coupled with PCR and Electrochemical Detection

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