Electrode design improvement for impedance evaluation of biological cell culture under variable frequency low intensity sinusoidal electric field

Chaudhuri, Chirasree Roy; Mondal, Debasis

doi:10.1109/tdei.2013.6508738

Cited by 7 publications

(7 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These dimensions have been selected based on the optimized geometry in the ECIS platform. 18 Finally, a polydimethylsiloxane (PDMS) well is fabricated by soft lithography technique and composed over the porous area for containing the cell suspension.…”

Section: A Ps Formation and Surface Modificationmentioning

confidence: 99%

“…Electrical measurement by impedance spectroscopy not only monitors cellular adhesion, spreading, and proliferation but also quantitatively estimates the average values of cell-cell junction resistance and cell membrane-substrate distance, along with their fluctuations due to micromotion. 18,19 However, these measurements have been carried out using electric cell substrate impedance sensing (ECIS) wells with patterned gold electrodes on plastic substrates whose topographies are not found in nature. 20 Hence, to mimic in vivo cellular responses, it is more appropriate to study the intra-and extracellular mechanisms on porous topographies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Real-time sensing of epithelial cell-cell and cell-substrate interactions by impedance spectroscopy on porous substrates

Mondal

Pal

RoyChaudhuri

2015

Journal of Applied Physics

Self Cite

View full text Add to dashboard Cite

Oxidized porous silicon (PS) is a common topographical biocompatible substrate that potentially provides a distinct in vitro environment for better understanding of in vivo behavior. But in the reported studies on oxidized PS, cell-cell and cell-substrate interactions have been detected only by fluorescent labeling. This paper is the first attempt to investigate real-time sensing of these interactions on HaCaT cells by label-free impedance spectroscopy on oxidized PS of two pore diameters (50 and 500 nm). One of the major requirements for successful impedance spectroscopy measurement is to restrict the channeling of electric field lines through the pores. To satisfy this criterion, we have designed the pore depths after analyzing the penetration of the medium by using computational fluid dynamics simulation. A distributed electrical model was also developed for estimating the various cellular attributes by considering a pseudorandom distribution of pores. It is observed from the impedance measurements and from the model that the proliferation rate increases for 50 nm pores but decreases for 500 nm pores compared to that for planar substrates. The rate of decrease in cell substrate separation (h) in the initial stage is more than the rate of increase in cell-cell junction resistance (Rb) corresponding to the initial adhesion phase of cells. It is observed that Rb and h are higher for 50 nm pores than those for planar substrates, corresponding to the fact that substrates more conducive toward cell adhesion encourage cell-cell interactions than direct cell-substrate interactions. Thus, the impedance spectroscopy coupled with the proposed theoretical framework for PS substrates can sense and quantify the cellular interactions.

show abstract

Section: A Ps Formation and Surface Modificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Real-time sensing of epithelial cell-cell and cell-substrate interactions by impedance spectroscopy on porous substrates

Mondal

Pal

RoyChaudhuri

2015

Journal of Applied Physics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The working electrode (WE) is around 250 µm in diameter and its spacing with the counter electrode (CE) is around 300 µm. These dimensions have been selected based on the optimized geometry in the ECIS platform reported earlier [21]. W Finally, a polydimethylsiloxane (PDMS) well has been fabricated by soft lithography technique and attached over the porous area of the sensor.…”

Section: A Sensor Fabricationmentioning

confidence: 99%

“…Solving the different balances in the crystallite region inside the WE [21], the potential (V 1 ) and current distribution (I c1 ) is given by equation 1:…”

Section: Analytical Model For Impedance Calculationmentioning

confidence: 99%

See 1 more Smart Citation

Real-Time Monitoring of Wound Healing on Nano-Patterned Substrates: Non-Invasive Impedance Spectroscopy Technique

Mondal

Bose

Chaudhuri

2016

IEEE Trans. Nanotechnology

View full text Add to dashboard Cite

Real time in vitro monitoring and impedance modeling of mammalian cell activities on planar ECIS and micro/nano patterned cytosensors

Mondal

RoyChaudhuri

2018

ISSS J Micro Smart Syst

View full text Add to dashboard Cite

Electrode design improvement for impedance evaluation of biological cell culture under variable frequency low intensity sinusoidal electric field

Cited by 7 publications

References 24 publications

Real-time sensing of epithelial cell-cell and cell-substrate interactions by impedance spectroscopy on porous substrates

Real-time sensing of epithelial cell-cell and cell-substrate interactions by impedance spectroscopy on porous substrates

Real-Time Monitoring of Wound Healing on Nano-Patterned Substrates: Non-Invasive Impedance Spectroscopy Technique

Real time in vitro monitoring and impedance modeling of mammalian cell activities on planar ECIS and micro/nano patterned cytosensors

Contact Info

Product

Resources

About