Electrodeposition-Assisted Rapid Preparation of Pt Nanocluster/3D Graphene Hybrid Nanozymes with Outstanding Multiple Oxidase-Like Activity for Distinguishing Colorimetric Determination of Dihydroxybenzene Isomers

Abstract

Here, we demonstrate a facile bottom-up strategy to fabricate Pt nanoclusters (Pt NCs) grafted onto three-dimensional graphene foam (3D GF) assisted by cetyltrimethyl ammonium bromide (CTAB) using the electrodeposition method. The homogeneous grafting of Pt NC onto 3D GF is due to the formation of hemimicelles above some CTAB concentration. With the unique nanocluster structure and the high content of Pt0, the Pt NC/3D GF nanohybrid exhibits extremely high activity and shows higher reusability and stability. Apart from the intrinsic oxidase-like activity with 3,3′,5,5′-tetramethylbenzidine (TMB) as the substrate, the Pt NC/3D GF nanohybrid can act simultaneously as an effective polyphenol oxidase (PPO) mimic, such as tyrosinase, catechol oxidase, and laccase. More importantly, utilizing intrinsic catechol oxidase-like activity and the oxidase-like activity with TMB as the substrate of the nanohybrid, distinguishing colorimetric determination of dihydroxybenzene isomers (catechol and hydroquinone) is performed. Distinguishing colorimetric analysis of dihydroxybenzene isomers was first developed using nanozymes. The present work provides a simple bottom-up approach for the reasonable fabrication of various nanostructured nanozymes with excellent performance using the electrodeposition method assisted with surfactants.

Mussel-Inspired Magnetic Nanoflowers as an Effective Nanozyme and Antimicrobial Agent for Biosensing and Catalytic Reduction of Organic Dyes

“…According to the previous research studies, the mechanism of peroxidase-like activity of nanoflowers could be attributed to the possibility that the nanoflowers promote electron transfer among H 2 O 2 and TMB (eq 5). 46 These results show that when the concentrations of nanoflowers increases, the adsorption intensity is also increased (Figure 6b). Antimicrobial Activity.…”

“…According to the previous research studies, the mechanism of peroxidase-like activity of nanoflowers could be attributed to the possibility that the nanoflowers promote electron transfer among H 2 O 2 and TMB (eq ). Huang et al investigated the peroxidase-like activity of the BSA Cu 3 (PO 4 ) 2 ·3H 2 O nanoflower and confirmed that the peroxidase-like activity was derived from the Cu 3 (PO 4 ) 2 ·3H 2 O crystal, whereas the BSA was used as an organic component to cause nucleation of Cu 3 (PO 4 ) 2 ·3H 2 O nanopetals. They also verified that the peroxidase-like activity was not due to free copper ion leaching during the reaction and relied on the nanoflower structure.…”

Prospects of nano-carbons as emerging catalysts for enzyme-mimetic applications

“…based carbon nanozymes has been reported by many groups and utilized further for detection, sensing, environmental application, etc. 89,149,150 The electrochemical sensing and The synergy between Pt NC and graphene foam provides the higher catalytic sites for the diffusion of substrates and accelerated electron transfer for redox reactions. This nanozyme shows excellent selectivity and high sensitivity for the catechol oxidation confirmed by mixing different phenols and ions into the solution.…”

“…This nanozyme shows excellent selectivity and high sensitivity for the catechol oxidation confirmed by mixing different phenols and ions into the solution. 152 A simple self-assembly-based fabrication of the 3D hydrogel using GO, poly(vinyl alcohol) (PVA) molecules, and G-quartet/ hemin (G4/H) motifs was reported by Zhang et al as an electrochemical sensor for the detection of H 2 O 2 to mimic peroxidase-like activity. 153 The GO/PVA/G4/H hydrogel was synthesized via cation-templated self-assembly, as shown in Fig.…”

MIL-100(Fe) Metal–Organic Framework Nanospheres Embedded in Graphene Matrixes for Xanthine Fluorescence Sensing

“…How to design and construct a favoring composition between MIL-100­(Fe) and GO? As a seamlessly connected porous carbon network, three-dimensional graphene (3DG) is an ideal substrate to accommodate catalysts. − More importantly, 3DG can avoid a loss of active sites and break the continuous pathway for electron/phonon transports from the agglomeration of 2D graphene sheets. , Up to date, many 3DG based-nanocomposites, for example, ZnFe 2 O 4 /ZnO/GO foam, Fe 3 O 4 NP-loaded 3DG, , Pt nanocluster/3DG, and 3DG@WO 3 , have been successfully fabricated and exhibited excellent catalytic activities. Considering the above facts, 3DG was chosen to combine with MIL-100­(Fe) based on the following reasons.…”