Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins

Bazin, Damien; Chevalier, Sébastien; Saadaoui, H.; Santarelli, Xavier; Larpent, Chantal; Feracci, Hélène; Faure, Chrystel

doi:10.1021/la301063s

Cited by 5 publications

(2 citation statements)

References 47 publications

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“…On the other hand, electrophoresis has been widely used to deposit colloidal particles onto a conducting substrate using direct-current (dc) electric field. However, this method usually results in densely packed layers with little control on the order of particle packing. − Liu et al fine-tuned the equilibrium distance of 2D colloidal arrays with both frequency of the ac electric field and temperature. The nonclose-packed array was further fixed by a sudden switch to a dc field.…”

Section: Introductionmentioning

confidence: 99%

Electric-Field Assisted Assembly of Colloidal Particles into Ordered Nonclose-Packed Arrays

Gong

2017

Langmuir

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Nonclose-packed colloidal arrays have many potential applications ranging from plasmonic sensors, light trapping for photovoltaics, to transparent electrodes. However, scalable fabrication of those structures remains a challenge. In this Article, we investigate the robustness of an electric-field assisted approach systematically. A monolayer of nonclose-packed crystalline array is first created under a low-frequency alternating-current electric field in solution. We then apply a sequence of direct-current pulses to fix the particle array onto the substrate so that it remains intact even after both field removal and solvent evaporation. Key process parameters such as the alternating-current field strength, direct-current magnitude, particle concentration, and solvent-evaporation rate that affect both ordering and fixing of colloidal particles have been studied systematically. We find that direct currents with an intermediate magnitude induce electrophoretic motion of particles toward the substrate and facilitate their permanent adhesion on the substrate due to strong van der Waals attraction. A higher current, however, causes lateral aggregation of particles arising from electroosmotic flow of solvent and destroys the periodic ordering between particles. This approach, in principle, can be conveniently adapted into the continuous convective assembly process, thus making the fabrication of nonclose-packed colloidal arrays scalable.

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Section: Introductionmentioning

confidence: 99%

Electric-Field Assisted Assembly of Colloidal Particles into Ordered Nonclose-Packed Arrays

Gong

2017

Langmuir

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“…Many biomolecules including proteins have been reported to be immobilized on surfaces with controlled orientation while maintaining their structure and functionality − by utilizing the formation of ternary metal–chellate complexes through the interaction of nitrotriacetic acid (NTA) and histidine-tagged (His-tag) biomolecules or exploitation of the streptavidin/biotin interaction.…”

Section: Introductionmentioning

confidence: 99%

Biofunctionality of Calmodulin Immobilized on Gold Surface Studied by Surface-Enhanced Infrared Absorption Spectroscopy: Ca²⁺-Induced Conformational Change and Binding to a Target Peptide

et al. 2016

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Calcium ion (Ca 2+ )-induced functional expression of calmodulin (CaM), which is a signal transmitter in living cell, is known to be associated with its conformation change; however, it is difficult to correlate the conformational change and functional expression induced by Ca 2+ reversibly as it is not easy to change the Ca 2+ concentration while keeping the CaM concentration in solution. This can be done if CaM is immobilized on a solid surface while maintaining its function intact. Here CaM is immobilized on a self-assembled monolayer (SAM) of Ni-nitrilotriacetic acid (NTA) on a Au surface by histidine tag (His-tag) method. The conformational change of the immobilized CaM induced by the Ca 2+ concentration change in solution was investigated using surface-enhanced infrared absorption spectroscopy (SEIRAS). The Ca 2+ -induced conformational change of CaM immobilized on the Au surface was followed by monitoring IR intensities at 1550 and 1645 cm −1 , which originated from the Ca 2+ binding site (EF hand) in CaM and the central helix portion in CaM, respectively. The conformation of CaM was confirmed to be changed reversibly when the concentration of Ca 2+ was increased and decreased between 10 −5 and 10 −3 M in a phosphate-buffered D 2 O solution. The isotherm for immobilized CaM with respect to the Ca 2+ concentration is in good agreement with that in solution, which confirms that the activity of CaM is maintained when immobilized on the surface. The interaction between CaM and a target peptide, mastoparan (MP), was also investigated using SEIRAS. Although MP was reversibly bound to and released from the CaM immobilized on the Au surface depending on the presence and absence of Ca 2+ , respectively, the dissociation constant of CaM immobilized on Au surface with MP was determined from the MP concentration dependence of attachment of MP as two orders of magnitude larger than that of CaM in homogeneous environment, possibly due to the difficulty for MP to access the binding site of CaM, which is immobilized on the surface with high order.

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Double‐Emulsion Globules as a Tool to Produce 2D Patterned Metal Deposits Using Electro‐Colloidal Lithography

Faure

Leal-Calderón

Saadaoui

2014

Adv Materials Inter

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micro-particles used to stabilize the emulsion translate to either the poles or the equator of the drops, depending on the relative dielectric constants of the particles, on the surrounding fl uid and on the fl uid within the drop. Such motions break the particle barrier, thus allowing the drops to merge and therefore the emulsion to be destabilized. In a different approach, Dommersnes et al. [ 4 ] demonstrated that AC fi elds could also induce the organization of colloidal particles (metallic, clays or polymers) in "equatorial" ribbons and "longitudinal" dipolar chains at the surface of silicone drops dispersed in castor oil.Oil-in-water emulsions (O/W) under electric fi eld were disregarded until recently since it is diffi cult to destabilize them electrically because of the high conductivity of the continuous phase: the current densities required to induce signifi cant chain formation are much larger compared to those required for W/O emulsions. Vigo and Ristenpart [ 5 ] recently developed a lowvoltage technique for separating stable O/W emulsions. They were able to induce lateral aggregation and further coalescence of the oil droplets by applying a transversal AC electric fi eld, thus proving that the aggregation behavior is strongly reminiscent of that observed under similar electric fi eld conditions for rigid colloids. [ 6 ] In the same vein, here we provide evidence that double-emulsion globules (W/O/W) behave exactly like solid colloidal particles, and that they can self-assemble into planar close-packed or non-close packed hexagonal crystals. Moreover, we take advantage of this property to produce patterned micro-structures using a process we recently developed, called electro-colloidal lithography. [ 7 ] Like conventional colloidal lithography, electro-colloidal lithography relies on the fabrication of a colloidal mask. In colloidal lithography, surface patterning combines colloidal crystal formation with other techniques such as etching, imprinting or electrochemical deposition of a metal salt. [ 8 ] The added-value of electro-colloidal lithography lies in its simplicity since both the colloidal mask and the patterned surface are generated by the application of electric fi elds. The process begins with the arrangement of polystyrene micro-beads into close-packed hexagonal crystals (hpc) or in non-close packed hexagonal crystals (hnpc) using an AC electric fi eld. After fi xing 2D-microbeads crystals onto the electrode surface, a second population of colloidal particles is introduced; either these particles A technique developed to self assemble solid colloidal particles under a sinusoidal electric fi eld (AC fi eld) is adapted to soft W/O/W double-emulsion globules, and is exploited for surface patterning. Double-emulsions containing cupric ions are prepared, placed between two planar ITO electrodes and submitted to a transversal AC fi eld which induced their ordering into hexagonal 2D-arrays. The characteristic spacing is monitored by varying the globule volume fraction. Such self-assembly is used...

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Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins

Cited by 5 publications

References 47 publications

Electric-Field Assisted Assembly of Colloidal Particles into Ordered Nonclose-Packed Arrays

Electric-Field Assisted Assembly of Colloidal Particles into Ordered Nonclose-Packed Arrays

Biofunctionality of Calmodulin Immobilized on Gold Surface Studied by Surface-Enhanced Infrared Absorption Spectroscopy: Ca²⁺-Induced Conformational Change and Binding to a Target Peptide

Double‐Emulsion Globules as a Tool to Produce 2D Patterned Metal Deposits Using Electro‐Colloidal Lithography

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Electrodeposition of Polymer Nanodots with Controlled Density and Their Reversible Functionalization by Polyhistidine-Tag Proteins

Cited by 5 publications

References 47 publications

Electric-Field Assisted Assembly of Colloidal Particles into Ordered Nonclose-Packed Arrays

Electric-Field Assisted Assembly of Colloidal Particles into Ordered Nonclose-Packed Arrays

Biofunctionality of Calmodulin Immobilized on Gold Surface Studied by Surface-Enhanced Infrared Absorption Spectroscopy: Ca2+-Induced Conformational Change and Binding to a Target Peptide

Double‐Emulsion Globules as a Tool to Produce 2D Patterned Metal Deposits Using Electro‐Colloidal Lithography

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Biofunctionality of Calmodulin Immobilized on Gold Surface Studied by Surface-Enhanced Infrared Absorption Spectroscopy: Ca²⁺-Induced Conformational Change and Binding to a Target Peptide