Electroelution of antigens immobilized on antibody-linked affinity matrices

Schulze‐Osthoff, Klaus; Michels, Elmar; Overwien, Bruno; Sorg, Clemens

doi:10.1016/0003-2697(89)90058-4

Cited by 3 publications

(2 citation statements)

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“…Electroelution is used routinely for recovery of small quantities of protein from aerylamide gels (Findlay 1990a, Otto andSnejdarkova 1981). Commercially available electroelution equipment has also been used for desorption of antigens from immunoaffinity gels (Schulze-Osthoff et al 1989). Electroelution cells are designed with small compartments, where proteins can be recovered in relatively high concentration.…”

Section: Immunoafiinity Chromatographymentioning

confidence: 99%

“…Findlay (1990a) has described a simple electroelution apparatus, which can be prepared using an electrophoresis lank and dialysis tubing. Schulze-Osthoff et al (1989) reported almost complete elution of BSA from BSA-antiserum-conjtigate gel after 4 h of electrophoresis (9 V cm'') in 10 mM Tris-borate buffer at pH 8.2 using the Biotrap apparatus.…”

Section: Immunoafiinity Chromatographymentioning

confidence: 99%

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Strategies in the purification of plant proteins

Weselake

Jain

1992

Physiologia Plantarum

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This review focuses on some recent developments in the purification of plant proteins. Topics discussed include chromatographic procedures using immobilized antibodies, protein subunits and metal ions. The use of affinity labeling to facilitate purification is presented along with recent uses of detergents. Some of the methodology has had limited application in plant biochemistry, and where appropriate, the authors have presented examples of results with non‐plant systems.

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Section: Immunoafiinity Chromatographymentioning

confidence: 99%

Section: Immunoafiinity Chromatographymentioning

confidence: 99%