Electrofusion of<i>Neurospora crassa</i>slime cells

Fikus, Magdalena; Grzesiuk, Elżbieta; Marszałek, Piotr E.; Rózycki, S.; Zielinski, Jerzy

doi:10.1111/j.1574-6968.1985.tb01651.x

Cited by 10 publications

(4 citation statements)

References 9 publications

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“…Similar values were found on Neurospora crassa slime cells using DEP 29 (membrane relative permittivity 8.3–9.4 and membrane conductivity < 1–2.2 × 10 –6 ) or dielectric spectroscopy 30 (membrane relative permittivity 7.9 and cytosol conductivity 0.23 S/m). When working on Myeloma Tib9 cells, Marszalek and col. 29 found a membrane relative permittivity of 9.4 and a membrane conductivity of less than 2 × 10 –6 .…”

Section: Resultssupporting

confidence: 78%

Early differentiation of mesenchymal stem cells is reflected in their dielectrophoretic behavior

Tivig,

Vallet,

Moisescu

et al. 2024

Sci Rep

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The therapeutic use of mesenchymal stem cells (MSCs) becomes more and more important due to their potential for cell replacement procedures as well as due to their immunomodulatory properties. However, protocols for MSCs differentiation can be lengthy and may result in incomplete or asynchronous differentiation. To ensure homogeneous populations for therapeutic purposes, it is crucial to develop protocols for separation of the different cell types after differentiation. In this article we show that, when MSCs start to differentiate towards adipogenic or osteogenic progenies, their dielectrophoretic behavior changes. The values of cell electric parameters which can be obtained by dielectrophoretic measurements (membrane permittivity, conductivity, and cytoplasm conductivity) change before the morphological features of differentiation become microscopically visible. We further demonstrate, by simulation, that these electric modifications make possible to separate cells in their early stages of differentiation by using the dielectrophoretic separation technique. A label free method which allows obtaining cultures of homogenously differentiated cells is thus offered.

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Section: Resultssupporting

confidence: 78%

Early differentiation of mesenchymal stem cells is reflected in their dielectrophoretic behavior

Tivig,

Vallet,

Moisescu

et al. 2024

Sci Rep

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show abstract

“…The alignment and fusion of A. nidulans protoplasts required stronger electric fields than for fusion of plant [2] or animal cells [3]. However, parameters were similar to those required for yeast protoplasts [6], N. crassa slime cells [8] and P. chrysogenum protoplasts. Membrane breakdown voltages required for fusion have been reported to be directly proportional to the radius of a cell [14] assuming that membrane breakdown occurs at all sites simultaneously.…”

Section: Resultsmentioning

confidence: 99%

“…The method has been very successfully applied to animal cells [3], plant protoplasts [4,5] and yeast protoplasts [6]. However, the filamentous fungi have been poorly represented although electrofusions between Penicillium chrysogenum protoplasts [7] and Neurospora crassa slime cells [8] have been reported.…”

Section: Introductionmentioning

confidence: 99%

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Electrofusion of protoplasts from Aspergillus nidulans

Lynch

1989

FEMS Microbiology Letters

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Electrofusion of protoplasts from celery (Apium graveolens L.) with protoplasts from the filamentous fungus Aspergillus nidulans

Lynch

Isaac

Collin

1989

Planta

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A method was developed for electrofusion of higher-plant protoplasts from celery and protoplasts from the filamentous fungus Aspergillus nidulans. Initially, methods for the fusion of protoplasts from ecch species were determined individually and, subsequently, electrical parameters for fusion between the species were determined. Pronase-E treatment and the presence of calcium ions markedly increased celery protoplast stability under the electrical conditions required and increased fusion frequency with A. nidulans protoplasts. A reduction in protoplast viability was observed after electrofusion but the majority of the protoplasts remained viable over a 24-h incubation period. A small decline in protoplast respiration rate occurred during incubation but those celery protoplasts fused with A. nidulans protoplasts showed elevated respiration rates for 3 h after electrofusion.

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Electrofusion ofNeurospora crassaslime cells

Cited by 10 publications

References 9 publications

Early differentiation of mesenchymal stem cells is reflected in their dielectrophoretic behavior

Early differentiation of mesenchymal stem cells is reflected in their dielectrophoretic behavior

Electrofusion of protoplasts from Aspergillus nidulans

Electrofusion of protoplasts from celery (Apium graveolens L.) with protoplasts from the filamentous fungus Aspergillus nidulans

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