Electrogenerated chemiluminescence aptamer-based biosensor for the determination of cocaine

Li, Yan; Qi, Honglan; Peng, Yage; Yang, Jia; Zhang, Chengxiao

doi:10.1016/j.elecom.2007.07.038

Cited by 128 publications

(77 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[16][17][18] But the combination of high sensitive ECL technique with an aptamer was seldom recorded in literatures. [19][20][21] To the best of our knowledge, ECL-based aptasensor for the determination of thrombin is rarely reported. 22 The combination of nanotechnology and biotechnology for the detection of biological elements has brought new opportunities.…”

Section: Introductionmentioning

confidence: 98%

Ru(bpy)₃²⁺‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

et al. 2008

View full text Add to dashboard Cite

A new electrogenerated chemiluminescent (ECL) detection system for protein using the aptamers was developed. Two different aptamers, which recognize different positions of thrombin, were chosen to construct sandwich type sensing system for protein, one was immobilized onto the gold electrode for capturing thrombin onto the electrode and the other was used for detection. To obtain the signal, the aptamer for detection was labeled with tris(2,2'-bipyridyl)ruthenium(II)-doped silica nanoparticles (

show abstract

Section: Introductionmentioning

confidence: 98%

Ru(bpy)₃²⁺‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

et al. 2008

View full text Add to dashboard Cite

show abstract

“…[25,26] Among different methods of fluorescence, [27] EC, [28] ECL [29] and colorimetry, [30,31] ECL has attracted substantial attention in the development of aptasensors because it is highly sensitive and inexpensive, and uses simple instruments. Traditionally, ECL aptasensors have used diverse luminophore-labeled aptamers, [32][33][34] which are difficult to obtain. Therefore, it is necessary to develop a simple and efficient method to fabricate aptasensors.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive and Signal‐on Electrochemiluminescence Aptasensor Using the Multi‐tris(bipyridine)ruthenium(II)‐β‐cyclodextrin Complexes

Zhao

Chen

et al. 2014

Chin. J. Chem.

View full text Add to dashboard Cite

An ultrasensitive and signal-on electrochemiluminescence (ECL) aptasensor to detect target protein (thrombin or lysozyme) was developed using the host-guest recognition between a metallocyclodextrin complex and single-stranded DNA (ss-DNA). The aptasensor uses both the photoactive properties of the metallocyclodextrins named multi-tris(bipyridine)ruthenium(II)-β-cyclodextrin complexes and their specific recognition with ss-DNA, which amplified the ECL signal without luminophore labeling. After investigating the ECL performance of different multi-tris(bipyridine)ruthenium(II)-β-cyclodextrin (multi-Ru-β-CD) complexes, tris-tris(bipyridine)-ruthenium(II)-β-cyclodextrin (tris(bpyRu)-β-CD) was selected as a suitable host molecule to construct an atasensor. First, double-stranded DNA (ds-DNA) formed by hybridization of the aptamer and its target DNA was attached to a glassy carbon electrode via coupling interaction, which showed low ECL intensity with 2-(dibutylamino) ethanol (DBAE) as coreactant, because of the weak recognition between ds-DNA and tris(bpyRu)-β-CD. Upon addition of the corresponding protein, the ECL intensity increased when target ss-DNA was released because of the higher stability of the aptamer-protein complex than the aptamer-DNA one. A linear relationship was observed in the range of 0.01 pmol/L to 100 pmol/L between ECL intensity and the logarithm of thrombin concentrations with a limited detection of 8.5 fmol/L (S/N＝3). Meanwhile, the measured concentration of lysozyme was from 0.05 pmol/L to 500 pmol/L and the detection limit was 33 fmol/L (S/N＝3). The investigations of proteins in human serum samples were also performed to demonstrate the validity of detection in real clinical samples. The simplicity, high sensitivity and specificity of this aptasensor show great promise for practical applications in protein monitoring and disease diagnosis.

show abstract

“…The Ru-peptide2 was purified by dialysis for 12 h at 4°C using MD34-2 Da molecular weight cutoff membrane with 0.1 M PBS (pH 7.4). The concentration of Ru-peptide2 solution was estimated to be 1.5×10 −5 M, on the basis of UV absorbance of Ru(bpy) 2 (dcbpy-NHS)(PF 6 ) 2 at 457 nm [32,33].…”

Section: Preparation Of Ecl Probesmentioning

confidence: 99%

Sensitive electrogenerated chemiluminescence peptide-based biosensor for the determination of troponin I with gold nanoparticles amplification

Meng

Qiu

et al. 2013

Gold Bull

Self Cite

View full text Add to dashboard Cite

A sensitive electrogenerated chemiluminescence (ECL) peptide-based biosensor was fabricated for the determination of troponin I (TnI) by employing gold nanoparticles as amplification platform. Two specific peptides including peptide1 with a sequence of CFYSHSFHENWPS and peptide2 with a sequence of FYSHSFHENWPSK were employed as capture peptide and report peptide, respectively. The peptide2 was labeled with ruthenium bis(2,2′-bipyridine) (2,2′-bipyridine-4,4′-dicarboxylic acid)-N-hydroxysuccinimide ester (Ru(bpy) 2 (dcbpy)NHS) at NH 2 -containing lysine via acylation reaction and utilized as the ECL probe. Gold nanoparticles were electrodeposited onto gold electrode and used as an amplification platform. The peptide-based biosensor was fabricated by self-assembling peptide1 onto the surface of gold nanoparticles-modified gold electrode through a thiolcontaining cysteine at the end of the peptide1. When the biosensor reacted with target TnI, and then incubated with the ECL probe, a strong ECL response was electrochemically generated. The ECL intensity is directly proportional to the logarithm of the concentration of TnI in the range from 1 to 300 pg/mL. The biosensor employing gold nanoparticles as amplification platform shows high sensitivity for the detection of TnI with a detection limit of 0.4 pg/mL (S/N =3). Moreover, the biosensor is successfully applied to analysis of TnI in human serum sample. This work demonstrates that the combination of a highly binding peptide with nanoparticle amplification is a great promising approach for the design of ECL biosensor.

show abstract

Electrogenerated chemiluminescence aptamer-based biosensor for the determination of cocaine

Cited by 128 publications

References 28 publications

Ru(bpy)₃²⁺‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

Ru(bpy)₃²⁺‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

Ultrasensitive and Signal‐on Electrochemiluminescence Aptasensor Using the Multi‐tris(bipyridine)ruthenium(II)‐β‐cyclodextrin Complexes

Sensitive electrogenerated chemiluminescence peptide-based biosensor for the determination of troponin I with gold nanoparticles amplification

Contact Info

Product

Resources

About

Electrogenerated chemiluminescence aptamer-based biosensor for the determination of cocaine

Cited by 128 publications

References 28 publications

Ru(bpy)32+‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

Ru(bpy)32+‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

Ultrasensitive and Signal‐on Electrochemiluminescence Aptasensor Using the Multi‐tris(bipyridine)ruthenium(II)‐β‐cyclodextrin Complexes

Sensitive electrogenerated chemiluminescence peptide-based biosensor for the determination of troponin I with gold nanoparticles amplification

Contact Info

Product

Resources

About

Ru(bpy)₃²⁺‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence

Ru(bpy)₃²⁺‐doped Silica Nanoparticle Aptasensor for Detection of Thrombin Based on Electrogenerated Chemiluminescence