Electroinduction of interferon-like proteins

Pretnar, G.; Filipič, Bratko; Golob, A; Škodič, A.; Tóth, Szabolcs; Mécs, I; Suhar, A.

doi:10.1016/0302-4598(91)87002-x

Cited by 9 publications

(2 citation statements)

References 18 publications

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“…Pretnar et al (19) reported that one or a few strong (700-1200-V) electric impulses can induce an IFN-like protein in human leukocytes that is antigenically different from any known antiviral molecule. Blood was obtained from the slaughterhouse, where the pigs were paralyzed by 3000 V of electroshock, and it was postulated that this could have been responsible for the presence of the antiviral agent.…”

Section: Resultsmentioning

confidence: 98%

Induction of Antiviral Response by Electric Pulses

Kovács¹,

Somogyvári

Filipič

et al. 2000

Electro- and Magnetobiology

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Antiviral activity was detected in porcine leukocytes obtained from animals killed in a slaughterhouse. The activity appeared in the supernatant immediately after the addition of Sendai virus, an intended interferon (IFN) inducer. Electroinduction appears to be responsible for the production of antiviral molecules in both porcine and human leukocytes. The macromolecule conferring the antiviral activity is sensitive to heat and pH 2.0 treatment. The preformed or electroinduced antiviral activity originating from porcine leukocytes was not neutralized by either anti-PoIFN-α or anti-PoIFN-γ. The substance involved differs from all known antiviral proteins in that it is not secreted spontaneously into the extracellular space. Its release requires intact and virulent Sendai virus as a trigger signal. Differences in antigenic properties and characteristics indicate that this is a new antiviral substance.

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Section: Resultsmentioning

confidence: 98%

Induction of Antiviral Response by Electric Pulses

Kovács¹,

Somogyvári

Filipič

et al. 2000

Electro- and Magnetobiology

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“…This collapses topically to 0 at the breached epithelium giving rise to a steady voltage gradient of 40 to 200 mV/mm directed toward the wound edge and parallel to the epithelial layer, with the wound negative about distal tissue. [3][4][5] Pretnar and colleagues 6 reported that one or few strong electric impulses could induce interferon (IFN)-like proteins in human leukocytes that are antigenically different from any contemporary IFN molecule. Kovacs and colleagues 7 found that in porcine leukocytes, the addition of Sendai virus and electric impulses produced porcine interferon a-like molecules.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Effect of 100.414-kHz Electromagnetic Field Produced by Defender’s Pulse Generator on the ChIFN γ-Like Molecule Inducing Capacity of Lens culinaris Agglutinin and 10% PBS Washouts of Different Holocene Minerals

Filipič¹,

Rihar²,

Gregorič³

et al. 2019

Technol Cancer Res Treat

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Macrophages play key role in host defense and tissue repair, and thus understanding regulation of their function is important. For instance, our previous results have shown that in chicken macrophage system (CoMA cell line), application of a pulse of electromagnetic fields of frequencies 0.618, 1.054, 5.229, and 100.414 kHz induces production of interferon γ-like molecules. In this study, we have shown that the electromagnetic field of 100.414 kHz is the most effective in inducing synthesis of chicken interferon γ and chicken interferon γ-like molecules in CoMA cells, especially when combined with Lens culinaris agglutinin and 10% phosphate-buffered saline washouts of different Holocene minerals. A 2-minute pulse of electromagnetic field was produced by Defender’s pulse generator. Both chicken interferon γ and chicken interferon γ-like molecules from the cell supernatant were evaluated by an antiviral assay and were also analyzed with reverse-phase high-performance liquid chromatography on Phenomenex, Aeris peptide columns. Our results show that application of a single inducing factor (Lens culinaris agglutinin, 100.414 kHz electromagnetic field, 10% phosphate buffer saline washout) or combined usage of 2 of them moderately stimulated production of chicken interferon γ-like molecules (from 1.550 to 48.028 IU/mL), whereas the combination of 10% phosphate-buffered saline washout of Koprivnica rock + Lens culinaris agglutinin + 100.414 kHz/9 V resulted in an output of 162.122 IU/mL. Hence, we may conclude that a combined use of electromagnetic field, Holocene minerals, and Lens culinaris agglutinin greatly stimulates synthesis of chicken interferon γ-like molecules in CoMA cells.

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Direct electric field effects and sequential processes in biosystems

Astumian

Berg²

1991

Journal of Electroanalytical Chemistry and Interfacial Electroc

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Electroinduction of interferon-like proteins

Cited by 9 publications

References 18 publications

Induction of Antiviral Response by Electric Pulses

Induction of Antiviral Response by Electric Pulses

Enhancing Effect of 100.414-kHz Electromagnetic Field Produced by Defender’s Pulse Generator on the ChIFN γ-Like Molecule Inducing Capacity of Lens culinaris Agglutinin and 10% PBS Washouts of Different Holocene Minerals

Direct electric field effects and sequential processes in biosystems

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