Electrokinetic behaviour of colloidal particles in travelling electric fields: studies using yeast cells

Huang, Ying; Wang, X B; Tame, John A.; Pethig, Ronald

doi:10.1088/0022-3727/26/9/030

Cited by 143 publications

(121 citation statements)

References 9 publications

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“…Cells suspended above TWD electrodes would be induced to move along the electrodes, at right-angles to the electrode orientation, and the velocity and direction of movement are related to the electrical properties of the cells. Demonstrations were performed of the separation of live and dead cells 18 with the two populations heading to opposite ends of the array; similarly, blood cell populations were separated by fractionation, with subpopulation of cells moving along the array at different speeds. 19 The use of TWD as a general motive force led to the development of putative "biofactories on a chip," highly complicated devices where railway tracks of electrodes would peel away from one another, sending populations in different directions.…”

Section: The Microfabrication Explosionmentioning

confidence: 99%

Fifty years of dielectrophoretic cell separation technology

Hughes

2016

Biomicrofluidics

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In 1966, Pohl and Hawk [Science 152, 647–649 (1966)] published the first demonstration of dielectrophoresis of living and dead yeast cells; their paper described how the different ways in which the cells responded to an applied nonuniform electric field could form the basis of a cell separation method. Fifty years later, the field of dielectrophoretic (DEP) cell separation has expanded, with myriad demonstrations of its ability to sort cells on the basis of differences in electrical properties without the need for chemical labelling. As DEP separation enters its second half-century, new approaches are being found to move the technique from laboratory prototypes to functional commercial devices; to gain widespread acceptance beyond the DEP community, it will be necessary to develop ways of separating cells with throughputs, purities, and cell recovery comparable to gold-standard techniques in life sciences, such as fluorescence- and magnetically activated cell sorting. In this paper, the history of DEP separation is charted, from a description of the work leading up to the first paper, to the current dual approaches of electrode-based and electrodeless DEP separation, and the path to future acceptance outside the DEP mainstream is considered.

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Section: The Microfabrication Explosionmentioning

confidence: 99%

Fifty years of dielectrophoretic cell separation technology

Hughes

2016

Biomicrofluidics

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“…This work led to Fuhr et al [1991] effectively rediscovering asynchronous travelling wave dielectrophoresis. Since then, a large corpus of study has been conducted on this technique, including theoretical studies (Huang et al [1993]; Hughes et al [1996]) and demonstrations of practical devices for electrostatic pumping (e.g. Fuhr et al 1994) and biotechnological applications (e.g.…”

Section: Travelling-wave Dielectrophoresismentioning

confidence: 99%

AC electrokinetics: applications for nanotechnology

Hughes¹

2000

Nanotechnology

266

177

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The phenomena of dielectrophoresis and electrorotation, collectively referred to as AC electrokinetics, have been used for many years to study, manipulate and separate particles on the nanotechnology, that is the precise manipulation of particles on the nanometre scale. In this paper we present the principles of AC electrokinetics for particle manipulation, review the current state of AC Electrokinetic techniques for the manipulation of particles on the nanometre scale, and consider how these principles may be applied to nanotechnology.3

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“…[20][21][22][23] ͒ The mechanism responsible for particle motion can therefore on occasion be ambiguous. The analysis presented in this paper will aid in discriminating between particle motion induced by dielectrophoresis and particle motion due to fluid flow in traveling-wavedielectrophoretic experiments.…”

Section: Introductionmentioning

confidence: 99%

Pumping of liquids with traveling-wave electroosmosis

Ramos

Morgan

Green

et al. 2005

Journal of Applied Physics

163

180

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Net flow of electrolyte induced by a traveling-wave electric potential applied to an array of microelectrodes is reported. Two fluid flow regimes have been observed: at small-voltage amplitudes the fluid flow follows the direction of the traveling wave, and at higher-voltage amplitudes the fluid flow is reversed. In both cases, the flow seems to be driven at the level of the electrodes. The experiments have been analyzed with a linear electroosmotic model based upon the Debye-Huckel theory of the double layer. The electrical problem for the experimental interdigitated electrode array is solved numerically using a truncated Fourier series. The observations at low voltages are in qualitative accordance with the electroosmotic model.

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Electrokinetic behaviour of colloidal particles in travelling electric fields: studies using yeast cells

Cited by 143 publications

References 9 publications

Fifty years of dielectrophoretic cell separation technology

Fifty years of dielectrophoretic cell separation technology

AC electrokinetics: applications for nanotechnology

Pumping of liquids with traveling-wave electroosmosis

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