Electron Beam Sterilization of Poly(Methyl Methacrylate)—Physicochemical and Biological Aspects

Abstract

Electron beam (E‐beam) irradiation is an attractive and efficient method for sterilizing clinically implantable medical devices made of natural and/or synthetic materials such as poly(methyl methacrylate) (PMMA). As ionizing irradiation can affect the physicochemical properties of PMMA, understanding the consequences of E‐beam sterilization on the intrinsic properties of PMMA is vital for clinical implementation. A detailed assessment of the chemical, optical, mechanical, morphological, and biological properties of medical‐grade PMMA after E‐beam sterilization at 25 and 50 kiloGray (kGy) is reported. Fourier transform infrared spectroscopy, thermogravimetric analysis, and differential scanning calorimetry studies indicate that E‐beam irradiation has minimal effect on the chemical properties of the PMMA at these doses. While 25 kGy irradiation does not alter the mechanical and optical properties of the PMMA, 50 kGy reduces the flexural strength and transparency by 10% and 2%, respectively. Atomic force microscopy demonstrates that E‐beam irradiation reduces the surface roughness of PMMA in a dose dependent manner. Live‐Dead, AlamarBlue, immunocytochemistry, and complement activation studies show that E‐beam irradiation up to 50 kGy has no adverse effect on the biocompatibility of the PMMA. These findings suggest that E‐beam irradiation at 25 kGy may be a safe and efficient alternative for PMMA sterilization.

Systematic optimization of visible light-induced crosslinking conditions of gelatin methacryloyl (GelMA)

“…Cell viability. The viability of cells was assessed using a commercial kit (LIVE/DEAD™ viability/cytotoxicity kit, for mammalian cells, (Thermofisher Scientific; Cambridge, MA), as previously described 60 , 61 . Briefly, cells were double-stained with calcein acetoxymethyl and ethidium homodimer for 30 min and imaged by inverted fluorescent microscopy (Zeiss Axio Observer Z1; Thornwood, NY) with a 10X objective.…”

Electron beam irradiation treatment of textiles materials: a review

“…This is crucially important in the radiation chemistry of the polymer and leads to the industrial application of the radiation processing of polymer. Although cross-linking and degradation may take place to some extent at the same time, one of them must predominate [46][47][48][49].…”

Graphene-Lined Porous Gelatin Glycidyl Methacrylate Hydrogels: Implications for Tissue Engineering

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