Electron-cytochemical osmium-ferricyanide staining of cereal chloroplasts

Mosejev, V. V.; Romanovskaya, O. I.; Iljin, Viktor V.

doi:10.1007/bf01273252

Cited by 3 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Black filling of ER and desmotubule (Fig. 4a, c) is a normal phenomenon when osmium ferricyanide stain is used (Mosejev et al 1987). In all four species PR in BS chloroplasts was usually enlarged near mitochondria (Fig.…”

Section: Pr Contact With Plasmodesmata and Mitochondriamentioning

confidence: 90%

“…Two 2 mm 9 1 mm pieces were cut from each leaf and placed in the fixative [50 mM HEPES; pH 7.0, 2 % (w/v) paraformaldehyde and 2 % (v/v) glutaraldehyde] for 4 h with a fixative change after first 2 h. Postfixation in 2 % osmium tetroxide (aqueous solution) with 0.8 % potassium ferricyanide-a technique reported to preferentially stain PR and membranes of the chloroplast envelope (Lawton 1988;Mosejev et al 1987)-was conducted overnight. Next, the material was dehydrated in ethanol graded series, embedded in Spurr's resin, cut into ultrathin sections of 80 nm and immobilized on copper slot grids with a Leica ultramicrotome, and contrasted in uranyl acetate (2 % aqueous solution).…”

Section: Tem Protocolmentioning

confidence: 99%

See 1 more Smart Citation

The occurrence of chloroplast peripheral reticulum in grasses: a matter of phylogeny or a matter of function?

Szczepanik

Sowiński

2014

Acta Physiol Plant

View full text Add to dashboard Cite

The chloroplast peripheral reticulum (PR) is a structure of unknown function. Some authors postulated that it is a characteristic feature of C 4 plants, although it was reported from C 3 species as well. It is unknown whether the occurrence of PR follows a phylogenetic (it is found in clades containing C 4 species, regardless of the photosynthetic type) or functional (photosynthetic pathway dependent) pattern. Here, we present a phylogenetically controlled analysis of the occurrence, form and functional aspects of PR in grasses. The occurrence of the PR follows a functional and not a phylogenetic pattern. Its most elaborated form (PR type I) is a unique feature of C 4 species. Although PR was found in some of the studied C 3 grasses, it was always less developed than PR in the chloroplasts of Kranz mesophyll cells of C 4 species. The size of PR in C 4 plants was found to increase when the plants were grown under low light intensity. Additional observations, such as a negative correlation between PR size and chloroplast surface and PR occurrence in vicinity of mitochondria or plasmodesmata, suggest that PR may play some role in C 4 metabolism.

show abstract

Section: Pr Contact With Plasmodesmata and Mitochondriamentioning

confidence: 90%

Section: Tem Protocolmentioning

confidence: 99%

The occurrence of chloroplast peripheral reticulum in grasses: a matter of phylogeny or a matter of function?

Szczepanik

Sowiński

2014

Acta Physiol Plant

View full text Add to dashboard Cite

show abstract

Ultrastructure of chloroplast membranes in leaves of maize and ryegrass as revealed by selective staining methods

Lawton

1988

New Phytologist

View full text Add to dashboard Cite

S II M MARYLeaves of Lolium multiflorum Lam. and Zea mays L. of different ages xvere prepared tor 'ri':M by a number of cytochemical methods. After post-fixation in potassium ferricyanide xvith osmium tetroxide the peripheral reticulum in both mesophyll and bundle sheath chloroplasts of maize stain very clearly. Post-fixation xxith uranyl acetate, copper and lead salts also stains the chloroplast envelope rather than the internal thylakoid system. Differences betxxeen the mesophyll and bundle sheath chloroplasts of maize and betxveen those of ryegrass are revealed after post-fixation in zinc iodide xxith osmium tetroxide. After post-fixation in potassium iodide xvith osmium tetroxide the loeuli stain less intensely than in zine iodide. Malachite green added to glutaraldehyde at pM 7-0 enhances membrane staining in general and reveals large lipid drops in the stroma. At pH beloxv 60 only the 'A' spaee is stained. The 'A' space and the loculus are stained xvhen ethidium bromide is added to glutaraldehyde. The different reactions ofthe ehloroplast membranes probably reflect differences in ehemieal composition.

show abstract

Copper and Cadmium Tolerance, Uptake and Effect on Chloroplast Ultrastructure. Studies on Salix purpurea and Phragmites australis

Hakmaoui

Ater

Bóka

et al. 2007

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

We have compared the effect of toxic Cu and Cd concentrations on growth, metal accumulation, and chloroplast ultrastructure of willow (Salix purpurea L.) and reed [Phragmites australis (Cav.) Trin. ex Steud.]. After a 10-day treatment, both species have tolerated to some extent the lowest concentration of both metals; however, plant growth was strongly reduced at the highest Cu and Cd concentrations. These plants could be described as Cutolerant at the lowest concentration tested, showing a higher tolerance index in reed than in willow; in contrast, willow exhibited higher tolerance against Cd. Both plants appeared to be moderate root accumulators of Cu and Cd. Ultrastructural studies revealed special features that can provide some protection against heavy metals stress, such as ferritin aggregates in the stroma. In addition, Cu and Cd induced distortion of thylakoids, reduction of grana stacks, as well as an increased number and size of plastoglobuli and peripheral vesicles.

show abstract

Electron-cytochemical osmium-ferricyanide staining of cereal chloroplasts

Cited by 3 publications

References 8 publications

The occurrence of chloroplast peripheral reticulum in grasses: a matter of phylogeny or a matter of function?

The occurrence of chloroplast peripheral reticulum in grasses: a matter of phylogeny or a matter of function?

Ultrastructure of chloroplast membranes in leaves of maize and ryegrass as revealed by selective staining methods

Copper and Cadmium Tolerance, Uptake and Effect on Chloroplast Ultrastructure. Studies on Salix purpurea and Phragmites australis

Contact Info

Product

Resources

About