Electron microscope observations of African swine fever virus in tissue culture cells

Characterization of African swine fever virion proteins j5R and j13L: immuno-localization in virus particles and assembly sites.

“…The virus factory contains membrane elements of endoplasmic reticulum (ER) origin and virus particles at various stages of assembly (Moura-Nunes et al, 1975 ;Brookes et al, 1996 ;Rouiller et al, 1998). The particles leave the virus factory as a prelude to release from the cell by budding through the plasma membrane where the outer envelope is acquired (Breese & Deboer, 1966 ;Moura-Nunes et al, 1975).…”

African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified Virus

“…Although the sources of ASF virus causing many of these outbreaks have not been conclusively established, uncooked pork products from airports and seaports have been implicated (Peritz, 1980). The African swine fever virus replicates in the cytoplasm of infected ceils, is icosahedral in shape, and matures by budding through the plasma membrane (Breese & DeBoer, 1966;Moura Nunes et al, 1975;Els & Pini, 1977). The genome of ASF virus consists of a double-stranded DNA molecule with an approximate molecular weight of 100 x 106 (Enjuanes et al, 1976) and contains $1 nuclease-sensitive cross-links near the ends (Ortin et al, 1979).…”

The African Swine Fever Virus Virion Membrane Protein pE248R Is Required for Virus Infectivity and an Early Postentry Event

“…The icosahedral virus particle contains more than 50 polypeptides and is composed of several concentric domains, including an internal DNA-containing nucleoid surrounded by a protein layer designated the core shell, an inner envelope, and an outer icosahedral capsid (8,10,20). An additional membrane acquired by budding through the plasma membrane envelops the extracellular virion (14).…”