Electron microscopic cytochemistry of alkaline phosphatase

Although decarboxylases, particularly ornithine decarboxylase, are of considerable importance in cell metabolism, it has been impossible to demonstrate their activity histochemically, as this depends on trapping carbon dioxide at neutral pH values. A new reagent, lead hydroxyisobutyrate, has been shown capable of such trapping. It has been applied to the demonstration of ornithine decarboxylase activity in mouse kidney. Optimal concentrations of substrate, co-factor and trapping agent, as well as the pH optimum, have been determined for cryostat sections stabilized with a collagen polypeptide. The activity was inhibited by the specific ornithine decarboxylase inhibitor alpha-difluoromethyl ornithine.

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Changes in the activity and distribution of alkaline phosphatase(ALP) in primary cultured rat hepatocytes.

正男¹

1987

Acta hepatologica Japonica

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Electron microscopic cytochemistry of alkaline phosphatase

Cited by 3 publications

References 73 publications

Quantitative cytochemistry of alkaline phosphatase activity

Quantitative cytochemistry of alkaline phosphatase activity

A quantitative cytochemical method for ornithine decarboxylase activity.

Changes in the activity and distribution of alkaline phosphatase(ALP) in primary cultured rat hepatocytes.

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