Electron Microscopic Features of Nasal Mucosa Treated with Topical and Circumostial Injection of Mitomycin C

Ali, Mohammad Javed; Baig, Fahd; Lakshman, Mekala; Naik, Milind N.

doi:10.1097/iop.0000000000000205

Cited by 27 publications

(12 citation statements)

References 13 publications

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“…Under TEM, the cells were highly swollen with cytoplasm after the H 2 O 2 treatment, which may cause the synthesis of glycoproteins, steroid hormones and lipids, resulting in functional damage (Ali, Baig, Lakshman, & Naik, 2015). In the case of dead cells, the nuclear membrane was incomplete, the mitochondrial membrane in the cytoplasm was incomplete, and the ridge was blurred (Hochstrasser & Sedat, 1987).…”

Section: Discussionmentioning

confidence: 99%

Moringa oleifera leaf flavonoids protect bovine mammary epithelial cells from hydrogen peroxide‐induced oxidative stress in vitro

Liu

Wang

et al. 2020

Reprod Domestic Animals

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Bovine mammary epithelial cells (BMECs) of high‐producing dairy cows are subject to constant oxidative stress as a result of high metabolic rate and physiological adaptation to intensive farming. Moringa (Moringa oleifera) leaf has been proposed to have the antioxidant potential in scavenging free radicals due to the presence of flavonoids. In this study, we investigated the cytoprotective effects of moringa leaf flavonoids in alleviating oxidative stress in BMECs in vitro. Oxidative stress was established by exposing isolated BMECs to H2O2 for 2 hr. Doses of moringa leaf flavonoids were evaluated by treating BMECs for 12 hr. The optimal concentrations of H2O2 and moringa leaf flavonoids were 500 μmol/L and 1.0 mg/ml, respectively. The results showed that moringa leaf flavonoids increased the activities of superoxide dismutase, catalase, and glutathione peroxidase; and reduced malondialdehyde activity and intracellular accumulation of reactive oxygen species (ROS) in the H2O2‐induced oxidative stress system. A Hoechst33258 staining assay revealed that moringa leaf flavonoids decreased the apoptosis rate in BMECs, while leaving membrane integrity and nucleolar morphology unchanged. We concluded that moringa leaf flavonoids have the antioxidant capacity to effectively reduce oxidative stress in BMECs.

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Section: Discussionmentioning

confidence: 99%

Moringa oleifera leaf flavonoids protect bovine mammary epithelial cells from hydrogen peroxide‐induced oxidative stress in vitro

Liu

Wang

et al. 2020

Reprod Domestic Animals

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“…The nasal fibroblasts showed a dramatic structural response to MMC: Intracellular edema, pleomorphic and vesicular mitochondria, dilated smooth and rough endoplasmic reticulum, and chromatin condensation. [ 12 ]…”

Section: The Evidence: Does Mitomycin-c Really Help?mentioning

confidence: 99%

Mitomycin-C in dacryocystorhinostomy: From experimentation to implementation and the road ahead: A review

Nair

Ali

2015

Indian J Ophthalmol

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Dacryocystorhinostomy (DCR) is the procedure of choice in patients with epiphora due to primary acquired nasolacrimal duct obstruction. The evolution of surgical tools, fiber-optic endoscopes, effective anesthesia techniques, and the adjunct use of antimetabolites intraoperatively; namely mitomycin-C (MMC) have significantly contributed to the advancement of DCR surgery. MMC is a systemic chemotherapeutic agent derived from Streptomyces caespitosus that inhibits the synthesis of DNA, cellular RNA, and protein by inhibiting the synthesis of collagen by fibroblasts. Even the cellular changes in the human nasal mucosal fibroblasts induced by MMC at an ultrastructural level have been documented. There, however, seems to be a lack of consensus regarding MMC: The dosage, the route of delivery/application, the time of exposure and subsequently what role each of these variables plays in the final outcome of the surgery. In this review, an attempt is made to objectively examine all the evidence regarding the role of MMC in DCR. MMC appears to improve the success rate of DCR.

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“…It has been used widely in other ophthalmic procedures like glaucoma filtration surgery and pterygium excision to enhance surgical success. Concerning its application in DCR, Ali et al demonstrated that both topical and circumostial injection of MMC induced ultrastructural changes within the fibroblasts, in particular, intracellular edema, pleomorphic, and vesicular mitochondria, dilated smooth and rough endoplasmic reticulum, and chromatin condensation [18]. Laboratory test on primary cultures of human nasal mucosal fibroblasts showed that application of MMC 0.4 mg/ ml beyond 5 min and 0.5 mg/ml beyond 3 min caused extensive cell death when compared with controls.…”

Section: Discussionmentioning

confidence: 98%

Safety and efficacy of adjunctive intranasal mitomycin C and triamcinolone in endonasal endoscopic dacryocystorhinostomy

Cheng

Wong

et al. 2015

Int Ophthalmol

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One of the common causes of failure in dacryocystorhinostomy for nasolacrimal duct obstruction (NLDO) is mucosal scarring and fibrosis around the ostium. Steroid and mitomycin C (MMC) can potentially reduce scarring by their action on the inflammatory and proliferative phase of wound healing, respectively. The purpose of this study is to evaluate the safety and efficacy of combined usage of adjunctive MMC and intranasal triamcinolone (TA) in endonasal endoscopic dacryocystorhinostomy (EE-DCR). This is a retrospective interventional case series. All patients underwent mechanical EE-DCR in two regional hospitals in Hong Kong from January 2005 to December 2006 were included. All received intraoperative MMC application for 5 min and gelfoam soaked with TA onto the ostium. Main outcome measures include the anatomical and functional success rate at follow-up at least 6 months after operation. Other outcomes include complications occurred during and after operation. A total of 73 EE-DCR were performed in 69 patients. Three patients had simultaneous bilateral DCR; one had sequential DCRs for both sides. At the last follow-up, anatomical success was achieved in 68 cases (93 %) and both anatomical with functional success in 67 cases (92 %). No major complication was observed. Minor complications included asymptomatic mucosal adhesion between the nasal septum and lateral nasal wall in one patient and moderate secondary hemorrhage in another. EE-DCR with adjunctive MMC and TA is a safe and successful procedure for the treatment of NLDO.

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Electron Microscopic Features of Nasal Mucosa Treated with Topical and Circumostial Injection of Mitomycin C

Cited by 27 publications

References 13 publications

Moringa oleifera leaf flavonoids protect bovine mammary epithelial cells from hydrogen peroxide‐induced oxidative stress in vitro

Moringa oleifera leaf flavonoids protect bovine mammary epithelial cells from hydrogen peroxide‐induced oxidative stress in vitro

Mitomycin-C in dacryocystorhinostomy: From experimentation to implementation and the road ahead: A review

Safety and efficacy of adjunctive intranasal mitomycin C and triamcinolone in endonasal endoscopic dacryocystorhinostomy

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