Electron microscopic improvement in the study of diarrheagenicEscherichia coli

Humbert, Jeanne; Jouve, Mabel; Bouguénec, Chantal Le; Gounon, Pierre

doi:10.1002/(sici)1097-0029(20000515)49:4<383::aid-jemt7>3.0.co;2-r

Cited by 9 publications

(9 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DAEC, diffusely adhering Escherichia coli; EAggEC, enteroaggregative Escherichia coli; EIEC, enteroinvasive Escherichia coli. (Reproduced with permission from Humbert et al, 2000. ) calves (Butler and Clarke, 1994) and lambs (Hodgson, 1994).…”

Section: Enterotoxigenic E Colimentioning

confidence: 99%

See 1 more Smart Citation

Identification of virulence attributes of gastrointestinalEscherichia coliisolates of veterinary significance

DebRoy

Maddox

2001

Anim. Health. Res. Rev.

134

113

View full text Add to dashboard Cite

The pathogenic strains of Escherichia coli recovered from the intestinal tract of animals fall into categories called enterotoxigenic, enteropathogenic, enterohemorrhagic and necrotoxigenic. The other two categories, enteroinvasive and enteroaggregative, have not been reported in animals. The pathogenicity of these strains is determined by the presence of certain genes that encode adhesins and toxins, are generally organized in large blocks in chromosomes, large plasmids or phages, and are often transmitted horizontally between strains. In this review, we summarize current knowledge of the virulence attributes that determine the pathogenic potential of E. coli strains and the methods available to assess the virulence of the strains. We also discuss the clinical symptoms, the gross and histological lesions, and the molecular diagnostic methods our laboratories have implemented for detecting pathogenic strains of E. coli that are isolated from the gastrointestinal tract of animals.

show abstract

Section: Enterotoxigenic E Colimentioning

confidence: 99%

“…1. This diagram, modified from Humbert et al (2000) is based upon the model of pathogenesis published by Nataro and Kaper (1998).…”

Section: Introductionmentioning

confidence: 99%

Identification of virulence attributes of gastrointestinalEscherichia coliisolates of veterinary significance

DebRoy

Maddox

2001

Anim. Health. Res. Rev.

134

113

View full text Add to dashboard Cite

show abstract

“…An unusual feature of the afa-positive strains is their additional association with intestinal infections in children (17,19,37,38). These diarrhea-associated isolates are E. coli organisms of the diffusely adherent pathotype (DAEC) (26,35).…”

mentioning

confidence: 99%

Characterization of AfaE Adhesins Produced by Extraintestinal and Intestinal Human Escherichia coli Isolates: PCR Assays for Detection of Afa Adhesins That Do or Do Not Recognize Dr Blood Group Antigens

Bouguénec¹,

Lalioui²,

Merle³

et al. 2001

J Clin Microbiol

Self Cite

View full text Add to dashboard Cite

adhesins) on the human decay-accelerating factor (DAF) as a receptor, and others (Afa/Dr؊ adhesins) do not. Thus, the afa operons detected in this study were characterized by subtyping the afaE gene using specific PCRs. In addition, the DAF-binding capacities of as-yet-uncharacterized AfaE adhesins were tested by various cellular approaches. The afaE8 subtype (Afa/Dr ؊ adhesin) was found to predominate in afa-positive isolates from sepsis patients (75%); it was frequent in afa-positive pyelonephritis E. coli (55.5%) and absent from diarrhea-associated strains. In contrast, Afa/Dr ؉ strains (regardless of the afaE subtype) were associated with both diarrhea (100%) and extraintestinal infections (44 and 25% in afa-positive pyelonephritis and sepsis strains, respectively). These data suggest that there is an association between the subtype of AfaE adhesin and the physiological site of the infection caused by afa-positive strains.

show abstract

“…These bacteria are not true invasive pathogens, unlike Salmonella and Shigella. However, several reports demonstrate that various Afa strains are able to enter epithelial cells in vitro, and can enter differentiated enterocytes after infection of piglet ileal explants (Goluszko et al, 1997b;Guignot et al, 2001;Humbert et al, 2000;Jouve et al, 1997;Selvarangan et al, 2000). At least two Afa products (AfaD and AfaE) contribute to different internalization processes, demonstrating the complexity of the interactions between afa-expressing bacteria and epithelial cells.…”

Section: Article In Pressmentioning

confidence: 99%

Adhesins and invasins of pathogenic Escherichia coli

Bouguénec

2005

International Journal of Medical Microbiology

View full text Add to dashboard Cite

Electron microscopic improvement in the study of diarrheagenicEscherichia coli

Cited by 9 publications

References 78 publications

Identification of virulence attributes of gastrointestinalEscherichia coliisolates of veterinary significance

Identification of virulence attributes of gastrointestinalEscherichia coliisolates of veterinary significance

Characterization of AfaE Adhesins Produced by Extraintestinal and Intestinal Human Escherichia coli Isolates: PCR Assays for Detection of Afa Adhesins That Do or Do Not Recognize Dr Blood Group Antigens

Adhesins and invasins of pathogenic Escherichia coli

Contact Info

Product

Resources

About