Electron Microscopic Studies on the Identification of Secretory Granules in the Parathyroid Gland of Several Animals by Means of Acid Phosphatase Reaction

Effects of Ovariectomy on the Ultrastructure of the Parathyroid Gland of the Golden Hamster

“…Large secretory granules of 400-600 nm in diameter are also present in the chief cells of the control and ovariectomized golden hamsters. Our interpretation that such large secretory granules are thought to be storage granules which remain undischarged in the cells is supported by the observation that large secretory granules are acid phos phatase-negative [Setoguti and Goto, 1974;Shannon and Roth, 1974;Hayashi et al, 1980;Setoguti et al, 1980]. In the present study, numerous vesicles similar to those inside and outside large vacuolar bodies surround large secretory granules, and large vacuolar bodies con tain material similar to the contents of the secretory granule.…”

Ultrastructural localization of phosphatases in the rat parathyroid gland.

“…10 Inset). Some lipofuscin granules, which are usually located in small groups in the peripheral cytoplasm towards the pericapillary space, indicate moderate deposits as previously demonstrated by one of the present authors (SETOGUTI and GOTO, 1974), but there are lipofuscin granules which show no deposits (Fig. 10).…”

“…Most of these authors demonstrated remarkable enzyme activities mainly on the vascular wall within the interstitial tissue, but not always on the parenchymal cell itself. Recently, ultrastructural studies on acid phosphatase activity have been performed in the parathyroid gland of humans (SHANON and BOTH, 1974), several mammals and an urodele (SETOGUTI and GOTO, 1974), but the main aim of the studies has been limited to demonstrate lysosomes in the parenchymal cell, and other types of phosphatase activity have not yet been observed. The present study was undertaken to elucidate the ultrastructural localization pattern of certain phosphatase enzymes in the parathyroid gland of the rat which is the most useful laboratory animal in the investigation of this gland.…”

Effects of glucocorticoid on the ultrastructure of the mouse parathyroid gland.

“…x 49,000 (SETOGUTI and GOTO, 1974;SHANNON and ROTH, 1974;HAYASHI et al, 1980;SETOGUTI et al, 1980). In the present study, large secretory granules of 300-600 nm in diameter were also present in the chief cells of the control and dexamethasone-treated mice.…”

“…The multivesicular bodies in the parathyroid gland are described to be acid phosphatase-negative (SETOGUTI and GoTO, 1974;HAYASHI et al, 1980;SETOGUTi et al, 1980). It may be possible that some of the vesicles lying adjacent to the storage granule are incorporated into the latter, forming some multivesicular bodies, as previously suggested in the studies using mice ISONo et al, , 1981 and rabbits ISONo et al, 1982).…”