ELECTRON MICROSCOPY OF FINE STRUCTURE OF BORRELIA DUTTONII

Electron Microscopy of Axial Fibrils, Outer Envelope, and Cell Division of Certain Oral Spirochetes

“…'Most of these recent studies described the existence of a 1, 088IS'rTARITEN ANI) SOCRIANSKY more or less granulai cytolplasm containing clear central areas of a filamentous or reticular nature. 'T'his reticular area is not se)arated from the remaining cytop)lasm by any detectable membrane, and is thought to rel)resent a nu(cleoid body similar to that found in otheI bacteria (Kawata, 1957(Kawata, , 1961l'illot, 1963). Listgarten et al (1963) also noted in Treponema microdentiurn the existence of concentiic laminations in a few crosssections of the l)rotol)lasmic cylinder, the significance of which is not understood.…”

“…The controlled enzyme digestion of the outer envelope (Bradfield and Cater, 1952;Swain, 1955) or its dissolution with deoxycholate (Swain, 1957) allowed these investigators to demonstrate that in most species the fibrils were probably contained in the space between the lprotol)lasmic cylinder and the outer envelope. 'More recent studies of ultrathin sections supl)lied additional information on the fine structure of the plotoplasmic cylinder (Kawata, 1957(Kawata, , 1961Simpson and White, 1961;-Miller and Wilson, 1962;Ryter and Pillot, 1963;Listgarten, Loesche, and Socransky, 1963), and provided conclusive proof that the axial fibrils are located between the protoplasmic cylinder and the outer envelope. 'Most of these recent studies described the existence of a 1, 088IS'rTARITEN ANI) SOCRIANSKY more or less granulai cytolplasm containing clear central areas of a filamentous or reticular nature.…”

Fine Structure of the Reiter Treponeme as Revealed by Electron Microscopy Using Thin Sectioning and Negative Staining Techniques

“…Since Shinohara et al (26) observed the mitochondria-like structure in sections of Mycobacterium avium, the intracytoplasmic membrane system showing various configurations, such as parallel stacks of membranes, lamellar bodies and clusters of vesicles, was demonstrated in bacteria and streptomycetes during the past few years (1,3,5,12,13,11,28) It has been established that such membranous organelles are derived from the cytoplasmic membrane; however, as yet, little is known about their function. Kawata(n) first observed similar membranous organelles presenting membranous stacks and laminated bodies in spirochetes.…”

Borreliae, Human Relapsing Fever, and Parasite-Vector-Host Relationships

“…He saw a distinct cell membrane, threadlike fibrous and granular structures similar to the nuclear apparatus of bacteria, and dense areas resembling nuclear sites. He (174) believed that B. duttonii has a foamy envelope which is dissolved in sodium deoxycholate. Kawata observed 20 to 25 fibrils on the surface of the cell wall.…”