Fine Structure of the Reiter Treponeme as Revealed by Electron Microscopy Using Thin Sectioning and Negative Staining Techniques

Kawata, Tomio; Inoue, Toshinori

doi:10.1111/j.1348-0421.1964.tb00258.x

Cited by 13 publications

(2 citation statements)

References 22 publications

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“…Lipopolysaccharide is a compound from the outer envelope of gram-negative bacteria that is responsible for endotoxicity and antigenic specificity among gram-negative bacteria (12,30) and is readily extracted with hot phenol-water (2,30,31). Electron microscopy of Treponema has shown that this genus has an outer envelope similar to that of other gram-negative bacteria (1,10,11,14,17,18,(24)(25)(26). It was presumed, therefore, that phenol-water extraction of betahemolytic T. hyodysenteriae could yield lipopolysaccharide-like antigens that would make serological grouping of the organism possible.…”

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confidence: 99%

Serotypes of beta-hemolytic Treponema hyodysenteriae

Baum¹,

Joens²

1979

Infect Immun

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Cultures from 13 isolates of pathogenic, beta-hemolytic Treponema hyodysenteriae from 11 geographically separate outbreaks and 2 experimentally induced cases of swine dysentery were lyophilized and extracted with hot phenol-water. The resulting water phases were examined serologically with antisera produced in rabbits against whole-cell bacterins of the 13 isolates for evidence of antigenic classes within the species. Water-phase antigens gave precipitin reactions with homologous antisera. Results from cross-testing of each water phase with each antiserum showed four serologically distinct groups among the isolants examined.

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confidence: 99%

Serotypes of beta-hemolytic Treponema hyodysenteriae

Baum¹,

Joens²

1979

Infect Immun

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“…After incubation with tellurite, whole cells were fixed by addition of formalin at a final concentration of 10% and then suspended in distilled water. The whole-cell suspensions were negatively stained with 1% phosphotungstic acid adjusted to pH 7.4 with KOH as previously described (23).…”

Section: Methodsmentioning

confidence: 99%