Electron microscopy of Staphylococcus epidermidis fibril and biofilm formation using image-enhancing ionic liquid

Takahashi, Chisato; Kalita, Golap; Ogawa, Naoko; Moriguchi, Keiichi; Tanemura, Masaki; Kawashima, Yasunaru; Yamamoto, Hiromitsu

doi:10.1007/s00216-014-8391-6

Cited by 21 publications

(13 citation statements)

References 39 publications

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“…As observed by optical microscopy and scanning electron microscopy, they showed similar morphology, such as round and agglomerated shape ( Figure 2E, F, I and J ), which is in accordance with the previous report. 28 …”

Section: Resultsmentioning

confidence: 99%

Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction

Jiang¹,

Deng

Yang³

et al. 2018

IDR

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ObjectiveTo explore the composition of the ocular microbiome in normal subjects and patients with Meibomian gland dysfunction (MGD).Subjects and methodsSeventy subjects (140 eyes) were enrolled in our study. Signs of dry eye were evaluated and bacterial species in the conjunctival sac (CS) and Meibomian gland (MG) secretions were then identified by 16S rRNA gene sequencing. Additionally, 17 subjects (34 eyes) were further evaluated to determine differences in the microbiomes in the surface and deep layers of MG using a segmental secretion analysis.ResultsThe positive bacterial isolation rate was markedly higher in MG secretions than in the CS. The bacterial composition of the control and mild group was simple, whereas the composition of bacteria was more complex as the severity of MGD increased. The positive bacterial isolation rate and number of bacterial types were significantly higher in the severe MGD group than those in the control, mild and moderate MGD groups. Corynebacterium macginleyi was only detected in the severe MGD group, with an isolation rate of up to 26.3%. Furthermore, a new grading system for bacterial severity of MGD was proposed and the severity of MGD appeared to be positively correlated with a higher grade of bacterial severity. The segmental secretion analysis showed severe MGD had a significantly higher incidence of bacterial discordance rate.ConclusionThe severity of MGD was positively correlated with a higher isolation rate, a greater number of bacterial species, and a higher grade of bacterial severity, which implied that MGD might be correlated with bacterial changes. This study provided some basis for the indications of antibiotic in clinical practice.

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Section: Resultsmentioning

confidence: 99%

Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction

Jiang¹,

Deng

Yang³

et al. 2018

IDR

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“…In S. epidermidis, thick slime-like layer was observed. Rod-like structures emerging from cell wall interconnecting to other bacterial cells were also seen and this may be due to proteinaceous factors (Takahashi et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Virulence factors associated with Coagulase Negative Staphylococci isolated from human infections

et al. 2017

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Infections caused by commensal organisms by changing to infectious life style generate much challenge to the current treatment strategies. Coagulase Negative Staphylococci (CoNS) are one of them, with their coexisting biofilm forming and multiple antibiotic resistance properties form important agents of nosocomial infection. To evaluate species distribution, biofilm formation, and antibiogram, CoNS isolates from various clinical samples were isolated. The presence of biofilm and associated genes icaAB, aap, atlE, embp, bhp, and fbe in CoNS was screened by PCR. The biofilm chemical composition and its correlation with the genotypes were also analysed. Staphylococcus epidermidis (59%) was found to be the most prevalent CoNS species. Most of the CoNS isolates harboring biofilm gene showed carbohydrate-protein-eDNA biofilm, whereas carbohydrate-protein biofilms were also observed. High percentage of multiple drug resistance, and biofilm gene frequency among these CoNS isolates point towards the need of periodic surveillance as CoNS are recently identified to cause difficult to treat infections.

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“…31 Various SEM images of bacterial biofilms have indicated the potential existence of bacterial nanotubes as prominent bridges between cells. 31,54 Our preliminary SEM images of Staphylococcus epidermidis which was incubated under different timeframes, also showed the prevalent occurrence of nanotubes on different surfaces (Fig. S6-S9, ESI †), which appeared to bridge and connect cells.…”

Section: The Growth Of P Aeruginosa Biofilm Is Aided Via Bacterial Nmentioning

confidence: 74%

Bacterial nanotubes mediate bacterial growth on periodic nano-pillars

et al. 2020

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Electron microscopy of Staphylococcus epidermidis fibril and biofilm formation using image-enhancing ionic liquid

Cited by 21 publications

References 39 publications

Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction

Pathogens in the Meibomian gland and conjunctival sac: microbiome of normal subjects and patients with Meibomian gland dysfunction

Virulence factors associated with Coagulase Negative Staphylococci isolated from human infections

Bacterial nanotubes mediate bacterial growth on periodic nano-pillars

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