Electron Microscopy of Streptomyces Spore Morphology and Its Role in Species Differentiation

Tresner, H. D.; Davies, M.C.; Backus, E. J.

doi:10.1128/jb.81.1.70-80.1961

Cited by 110 publications

(33 citation statements)

References 9 publications

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“…and Benedict (5), and spore surface configuration was assessed by electron microscopy as described by Tresner et al (8). Carbon source utilization patterns were established by the methods of Pridham and Gottlieb (4); nitrate reduction was measured by the techniques described in A Manual of Methods for Pure Culture Study of Bacteria (1); and tolerance to a graded series of NaCI concentrations (4 to 13%) was determined in a yeast extract-agar medium, the formula of which is given by Pridham et al (3).…”

Section: Methodsmentioning

confidence: 99%

“…They defined the complex as consisting of streptomycetes which are characterized by (i) tightly spiralled spore chains, (ii) brownish-gray spore masses, and (iii) the formation of hygroscopic patches in the sporulating aerial mycelium. The complex might now be characterized further by adding that none of its members is chromogenic, i.e., darken peptoneiron agar (7), and that all of its members have smooth-surfaced spores (8). Dietz and Mathews (2) showed, however, that, by use of carbon repligraphic techniques, the spores exhibited intricate surface features, although they appeared smooth in silhouette with ordinary electron microscopy.…”

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confidence: 99%

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Morphological Spore Types in the Streptomyces hygroscopicus-like Complex

Tresner¹,

Backus²,

Hayes³

1967

Applied Microbiology

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Streptomycetes comprising the Streptomyces hygroscopicus-like complex may be divided into two groups based on the micromorphology of their spores. The species S. hygroscopicus has short-cylindrical, phalangiform spores, whereas members of the second group possess elliptical-type spores. It was determined that S. platensis, of the latter group, is not only readily distinguishable from S. hygroscopicus on the basis of spore micromorphology, but differs, as well, according to various physiological measurements.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

Morphological Spore Types in the Streptomyces hygroscopicus-like Complex

Tresner¹,

Backus²,

Hayes³

1967

Applied Microbiology

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“…CuOff24 was identified conventionally. [22][23][24][25] Furthermore, molecular identification of the potential isolates was achieved by 16S rRNA gene sequencing and phylogenetic analysis and evolutionary relationships were obtained using MEGA 6 software. 26…”

Section: Identification Of Potential Eps Producing Marine Streptomycementioning

confidence: 99%

Bioremediation of Sr²⁺ ion radionuclide by using marine Streptomyces sp. CuOff24 extracellular polymeric substances

Kannan

Sivaperumal

Thilagaraj

et al. 2019

J of Chemical Tech & Biotech

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BACKGROUND Microbial biosorption is recognized as a resourceful process for the remediation of pollution from contaminated environs. To investigate radionuclide bioremediation, carbohydrate contained extracellular polymeric substance (EPS) and strontium ion (Sr+) tolerant marine actinobacterium has been isolated and identified as Streptomyces sp. CuOff24 through 16S rDNA sequencing. RESULTS EPS was extracted, characterized and the biosorption of the long half‐life radionuclide of Sr2+ was also performed. EPS could remove 82% of Sr2+ from the experimental solution of 100 mg L−1 of strontium chloride (SrCl2). Furthermore, EPS composition also obtained with the presence of Sr2+ radionuclide and major contents of carbohydrate (68.6%) followed by protein (12.3%), nucleic acid (10.4%) and unidentified compounds 8.7%) were quantified. The spectroscopy study also confirmed the presence of carbohydrate functional groups presence in the EPS surface were probably to be involved in Sr2+ adsorption. CONCLUSION Overall results conclude that, marine actinobacterial EPS could be used for the large‐scale remediation of radionuclides contaminated environment. © 2019 Society of Chemical Industry

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“…According to Fig. 3a, S. lunalinharesii presents spore surface ornamentation belonging to the spiny group (Tresner et al, 1961).…”

Section: Spore Ornamentationmentioning

confidence: 99%

Streptomyces lunalinharesiispores contain chitin on the outer sheath

Gomes¹,

Soares²,

Nakamura³

et al. 2008

FEMS Microbiology Letters

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Chitin from Streptomyces lunalinharesii spores, detected on its outermost surface layer, was isolated and characterized by chemical and spectroscopic methods, transmission electron microscopy and flow cytometry analysis. Gold-chitinase- and gold-lectin (Lycopersicum esculentum agglutinin, LEA)-conjugated labels were used in microscopy experiments, whereas a fluorescence-lectin (LEA) conjugate was used in flow cytometry analysis. Chitin isolation consisted of several steps of hot alkali and nitrous acid treatment, and the final material was obtained in the colloidal form. The infrared and the 13C CP/MAS NMR spectra of Streptomyces sp. colloidal chitin and colloidal chitin obtained from commercial crab shell chitin were very similar. Incubation of the spores with gold-labeled lectin, or gold-labeled recombinant chitinase, showed the presence of gold particles around the spore surface, indicating the specific binding of the lectin or the recombinant chitinase with the chitin present on the outermost surface. Flow cytometry analysis, using the fluorescence-lectin conjugate, confirmed these results. According to scanning electron microscopy, S. lunalinharesii presented spore surface ornamentation belonging to the spiny group. This is the first detailed characterization of chitin on the spore's outermost layer from a Streptomyces species.

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Electron Microscopy of Streptomyces Spore Morphology and Its Role in Species Differentiation

Cited by 110 publications

References 9 publications

Morphological Spore Types in the Streptomyces hygroscopicus-like Complex

Morphological Spore Types in the Streptomyces hygroscopicus-like Complex

Bioremediation of Sr²⁺ ion radionuclide by using marine Streptomyces sp. CuOff24 extracellular polymeric substances

Streptomyces lunalinharesiispores contain chitin on the outer sheath

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Electron Microscopy of Streptomyces Spore Morphology and Its Role in Species Differentiation

Cited by 110 publications

References 9 publications

Morphological Spore Types in the Streptomyces hygroscopicus-like Complex

Morphological Spore Types in the Streptomyces hygroscopicus-like Complex

Bioremediation of Sr2+ ion radionuclide by using marine Streptomyces sp. CuOff24 extracellular polymeric substances

Streptomyces lunalinharesiispores contain chitin on the outer sheath

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Product

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Bioremediation of Sr²⁺ ion radionuclide by using marine Streptomyces sp. CuOff24 extracellular polymeric substances