Morphological Spore Types in the Streptomyces hygroscopicus-like Complex

Tresner, H. D.; Backus, E. J.; Hayes, Jean A.

doi:10.1128/aem.15.3.637-639.1967

Cited by 2 publications

(1 citation statement)

References 4 publications

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“…It has smooth-cylindrical spores which may be formed on the substrate or aerial mycelium as a straight chain to rectiflexible and are nonmotile. [15][16][17] The current research demonstrates the isolation, identification, characterization and anti-microbial screening of the Actinomycete strain Streptomyces polyrhachis AS07 from soil along with the production, fermentation and yield optimisation of the secondary metabolic extract. Microbial fermentation was employed to obtain a sufficient yield of the crude metabolite under appropriate growing conditions that facilitate optimal secondary metabolite synthesis.…”

Section: Introductionmentioning

confidence: 99%

Isolation, characterization, and identification of bioactive metabolite from soil derived Streptomyces polyrhachis AS07

2022

JABB

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The increasing stress caused by antibiotic resistance necessitates the need to explore novel and effective antimicrobial agents from actinomycetes which are often acknowledged for their capability to produce a variety of antibiotics. This study focuses on investigating the antimicrobial properties of the secondary metabolite synthesized by the soil resident Streptomyces polyrhachis AS07. The soil isolated strain was identified using 16S rRNA sequencing technology and phylogenetic analysis. Under appropriate laboratory conditions, the Streptomyces sp. produced sufficient yield of secondary metabolite for assessment of antimicrobial properties. The Gas Chromatography Mass Spectrometry chromatogram of the metabolite revealed abundant presence of antimicrobial constituents such as propionic acid, palmitic acid, and other compounds from tetrazolic and monocarboxylic groups. When tested against a broad range of Gram-positive and Gram-negative bacteria, the extracted metabolite exhibited appreciable antimicrobial activity with highest activity against S. aureus. Additionally, it also had potential antioxidant activity with an IC50 value of 5.50 μg/mL. The metabolite displayed highest antibiofilm activity against B. subtilis as well as successfully restricted the quorum sensing ability of C. violaceum. Furthermore, the extract significantly inhibited the swarming ability of P. aeruginosa upto 16.67 %. Conclusively, Streptomyces metabolite can be considered as a potent reservoir of bioactive molecules for industrial production with promising pharmaceutical applications.

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Section: Introductionmentioning

confidence: 99%