Electron-Opaque Bodies and Fat Droplets in Mouse Liver After Fasting or Glucose Injection

Trotter, Nancy L.

doi:10.1083/jcb.34.3.703

Cited by 18 publications

(4 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specialization of the smooth ER for synthesis of neutral lipid for export is suggested by numerous studies on fatty liver, the common early morphologic feature of which is the accumulation of triglyceride-rich particles within vesicles of smooth ER (9,16,23,27,52,53). In normal liver, particles the size of VLDL do not occur in the flattened cisternae of the rough ER except at the smoothsurfaced ends, some of which are sites of continuity with smooth ER (9,27).…”

Section: Discussionmentioning

confidence: 99%

Subcellular Localization of “B” Apoprotein of Plasma Lipoproteins in Rat Liver

Alexander¹,

Hamilton²,

Havel³

1974

Proc. annu. meet. Electron Microsc. Soc. Am.

View full text Add to dashboard Cite

The B apoprotein is thought to be essential for the synthesis and secretion of triglyceriderich lipoproteins from mammalian liver. Antibodies were prepared against rat low density lipoproteins (LDL, density 1.025-1.045) and very low density lipoproteins (VLDL). By Ouchterlony immunodiffusion and Immunoelectrophoresis, the major determinant shared by these antisera had the characteristics of the B apoprotein. Fab fragments were prepared by the method of Porter and conjugated to horseradish peroxidase (HRP) by the two step method of Avrameas and Ternynck. Male Long-Evans rats (250-300 gm) were fasted overnight, anesthetized with Brevital (Lilly), and perfused through the portal vein with fresh 4% formaldehyde in 0.135 M sodium phosphate buffer.

show abstract

Section: Discussionmentioning

confidence: 99%

Subcellular Localization of “B” Apoprotein of Plasma Lipoproteins in Rat Liver

Alexander¹,

Hamilton²,

Havel³

1974

Proc. annu. meet. Electron Microsc. Soc. Am.

View full text Add to dashboard Cite

show abstract

“…The BDNF and LR immunoreactivity was higher in young WT and 5-HTT ko mice after fasting (Figures 4a-h Obese 5-HTT ko mice have more pronounced hepatic steatosis than WT mice The increase in liver fat is part of the adaptive response to fasting. 21 We used oil red staining to investigate whether fasting-induced changes in liver fat are altered in 5-HTT ko mice. Fasting resulted in an increase in liver fat (Figures 5a and b) in both genotypes in young (WT: P ¼ 0.02, ko: P ¼ 0.02; Figure 5c) and old mice (WT: P ¼ 0.001, ko: P ¼ 0.04; Figure 5d).…”

Section: Resultsmentioning

confidence: 99%

Lack of the serotonin transporter in mice reduces locomotor activity and leads to gender-dependent late onset obesity

et al. 2010

View full text Add to dashboard Cite

Objective: Mice deficient of the serotonin transporter (5-HTT ko) mice have a reduced brain serotonin content and develop lateonset obesity. To elucidate the pathophysiology of this obesity, we analyzed the expression of the interrelated weight-regulatory molecules: brain-derived neurotrophic factor (BDNF) and leptin receptor (LR) in brain areas associated with nutrition and activity. Research Design and Methods: We investigated feeding behavior, physical activity and metabolic parameters of 5-HTT ko and wild-type mice and measured the expression of BDNF and LR in brain areas associated with nutrition and activity using quantitative real-time PCR. The influence of age, gender and fasting was analyzed. Results: Male 5-HTT ko mice developed obesity without hyperphagia from the age of 5 months. Physical activity was reduced in old male, but not old female, 5-HTT ko mice. The BDNF gene expression in frontal cortex was elevated in young, but reduced in old 5-HTT ko mice. Fasting failed to increase the BDNF gene expression in frontal cortex of young 5 HTT ko mice and in the hypothalamus in old 5-HTT ko mice. The fasting-induced hypothalamic increase of LR was absent in both young and old 5-HTT ko mice. Conclusions: We propose that low brain serotonin level due to the 5-HTT ko genotype leads to reduced physical activity and low BDNF, which together with the lack of fasting-induced hypothalamic BDNF and LR production results in late-onset obesity. Although lack of the 5-HTT is a genetic vulnerability factor for obesity, female gender is protective.

show abstract

Section: Figure 13mentioning

confidence: 99%

Subcellular localization of B apoprotein of plasma lipoproteins in rat liver.

Alexander¹,

Hamilton²,

Havel³

1976

The Journal of Cell Biology

343

159

View full text Add to dashboard Cite

Multispecific antigen-binding fragments (Fab) from rabbit antisera against rat very low density lipoproteins (VLDL) and Fab against rat low density lipoproteins that were monospecific for the B apoprotein were conjugated to horseradish peroxidase. Conjugates were incubated with 6-#m frozen sections from fresh and perfusionfixed livers and with tissue chopper sections (40 am thick) from perfusion-fixed livers. In the light microscope, specific reaction product was present in all hepatocytes of experimental sections as intense brown to black spots whose locations corresponded to the distribution of the Golgi apparatus: along the bile canaliculi, near the nuclei, and between the nuclei and bile canaliculi. Perfusion fixation with formaldehyde produced satisfactory ultrastructural preservation with retention of lipoprotein antigenic determinants. In the electron microscope, patches of cisternae and ribosomes of the rough endoplasmic reticulum (ER) and particularly its smooth-surfaced ends, vesicles located between the rough ER and the Golgi apparatus, the Golgi apparatus and its secretory vesicles and VLDL particles in the space of Disse all bore reaction product. The tubules and vesicles of typical hepatocyte smooth ER did not contain reaction product, nor did the osmiophilic particles contained therein. The localization obtained in this study together with other evidence suggests a sequence for the biosynthesis of VLDL that differs in some respects from that proposed by others: (a) the triglyceride-rich particle originates in the smooth ER where triglycerides are synthesized; (b) at the junction of the smooth and rough ER the particle receives apoproteins synthesized in the rough ER; (c) specialized tubules transport the particle, now a nascent lipoprotein, to the Golgi apparatus where concentration occurs in secretory vesicles; (a t) secretory vesicles move to the sinusoidal surface where the particles are secreted into the space of Disse by fusion of the vesicular membrane with the plasma membrane of the hepatocyte.Very low density lipoproteins (VLDL) ~ of vertebrate blood plasma are triglyceride-rich particles 1Abbreviations used in this paper." DAB, 3,3'-diaminobenzidine tetrahydrochloride; EDTA, disodium (ethylenedinitrilo)-tetraacetic acid; ER, endoplasmic reticulum; Fab, fragment antigen binding; Fc, fragment 250-800 ~ in diameter. Primarily, they transport triglycerides from liver to those tissues that store their constituent fatty acids or utilize them as fuel crystallizable; LDL, low density lipoproteins; HDL, high density lipoprotcins, VLDL, very low density lipoproteins.

show abstract

Electron-Opaque Bodies and Fat Droplets in Mouse Liver After Fasting or Glucose Injection

Cited by 18 publications

References 30 publications

Subcellular Localization of “B” Apoprotein of Plasma Lipoproteins in Rat Liver

Subcellular Localization of “B” Apoprotein of Plasma Lipoproteins in Rat Liver

Lack of the serotonin transporter in mice reduces locomotor activity and leads to gender-dependent late onset obesity

Subcellular localization of B apoprotein of plasma lipoproteins in rat liver.

Contact Info

Product

Resources

About