Electron probe X-ray microanalysis of human skeletal muscle involved in rheumatoid arthritis

Wróblewski, Romuald; Gremski, Waldemiro; Nordemar, Rolf; Edström, Lars

doi:10.1007/bf00507351

Cited by 21 publications

(4 citation statements)

References 13 publications

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“…It is possible that different kinds of degenerative and destructive changes affecting the myopathic muscle fibers could result in some of the ionic changes described here. It must, however, be emphasized that we have not seen such changes in other atrophic, centronucleated, or degenerated fibers investigated so far in muscle dystrophies, congenital myopathies, inflammatory myopathies, and disuse atrophy.g,1"*33, 35,36 We are aware that in the fibers of myotubular Figure 7. Characteristic x-ray microanalytical spectra from muscle in myotubular myopathy (A) and a control muscle (B).…”

Section: Discussionmentioning

confidence: 89%

Genuine myotubular myopathy

1982

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Two patients, a father and his 14-year-old son, were suffering from a facioperoneal syndrome, and muscle biopsy findings were consistent with a myotubular myopathy. The father exhibited central nuclei in most muscle fibers, but his son had typical changes exclusively in hypotrophic type I fibers. The cytochemical and ultrastructural analysis revealed a spectrum of pathological changes typical of myotubular myopathy. Energy-dispersive electron probe x-ray microanalysis was performed on 6- to 12-microns thick freeze-dried cryosections visualized in the scanning or scanning transmission mode of electron microscopy. We found a high intracellular sodium and chlorine concentration and a low potassium concentration in comparison with control muscles. These changes pointed in the direction similar to results from human fetal muscle. The changes in the intracellular elemental composition may indicate a membrane pump dysfunction, which might be caused by a partial arrest in muscle fiber maturation.

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Section: Discussionmentioning

confidence: 89%

Genuine myotubular myopathy

1982

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“…The total elemental content (both bound and ionic) is measured by this method. X-ray microanalysis has previously been used in healthy and diseased skeletal muscle from man, rats and cows (Wroblewski et a/. 1978;Edstrom et al 1979;Wroblewski and Edstrom 1983;Bjorkman et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Elemental composition of muscle at rest and potassium levels in muscle, plasma and sweat of horses exercising at 20°C and 35°C

Gottlieb-Vedi¹,

Dahlborn²,

Jansson³

et al. 1996

Equine Veterinary Journal

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Summary In this study, 4 Standardbred geldings were exercised at 20 and 35°C. The exercise test (ET) consisted of 2 exercise bouts separated by 2 h of rest in their boxes. Blood samples were taken before, during and after the second exercise bout and muscle (m. gluteus medius) biopsies were taken before the first exercise bout and after an intensive trot over 2600 m in the second exercise bout. The blood samples were analysed for plasma potassium and total plasma protein concentration (TPP) and the muscle fibres were analysed for elemental composition by x‐ray microanalysis. The intracellular content was as follows: sodium (Na) = 40 ± 7; magnesium (Mg) = 32 ± 4; phosphorus (P) = 282 ± 15; sulphur (S) = 222 ± 13; chloride (Cl) = 119 ± 31; potassium (K) = 304 ± 21 and calcium (Ca) = 8 ± 2 mmol/kg dry weight under resting conditions. Intracellular potassium content increased after exercise compared to resting values. There was a good correlation between exercise intensity, plasma potassium concentration and shifts in plasma volume, indicated by alterations in TPP. This probably reflects the very fast shift of potassium and fluid between muscle and plasma. Plasma potassium concentrations decreased below resting values post exercise. The higher dehydration degree and potassium sweat loss after exercise at 35°C was not reflected in lower muscular potassium content, but by a lower plasma potassium/total plasma protein ratio after exercise, indicating less circulating potassium.

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“…Generally, changes in catabolic responses between predominantly glycolytic or oxidative skeletal muscle types have been assessed in several non-RA models of chronic inflammation including burn-injury [30], chronic heart failure [31], and sepsis [32] with data suggesting that fast-twitch glycolytic muscles are more prone to atrophy. In RA specifically, early patient-based studies have reported the most severe atrophy in type II fibres from the quadriceps femoris muscle [33,34]. Of interest to the current review topic, some also reported increased collagen fibril deposition around fibres exhibiting mild necrosis (but not in fibres with severe atrophy or necrosis) [34].…”

Section: The Role Of Skeletal Muscle Fibre Type Compositionmentioning

confidence: 99%

Rheumatoid cachexia: the underappreciated role of myoblast, macrophage and fibroblast interplay in the skeletal muscle niche

et al. 2021

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Although rheumatoid arthritis affects 1% of the global population, the role of rheumatoid cachexia, which occurs in up to a third of patients, is relatively neglected as research focus, despite its significant contribution to decreased quality of life in patients. A better understanding of the cellular and molecular processes involved in rheumatoid cachexia, as well as its potential treatment, is dependent on elucidation of the intricate interactions of the cells involved, such as myoblasts, fibroblasts and macrophages. Persistent RA-associated inflammation results in a relative depletion of the capacity for regeneration and repair in the satellite cell niche. The repair that does proceed is suboptimal due to dysregulated communication from the other cellular role players in this multi-cellular environment. This includes the incomplete switch in macrophage phenotype resulting in a lingering pro-inflammatory state within the tissues, as well as fibroblast-associated dysregulation of the dynamic control of the extracellular matrix. Additional to this endogenous dysregulation, some treatment strategies for RA may exacerbate muscle wasting and no multi-cell investigation has been done in this context. This review summarizes the most recent literature characterising clinical RA cachexia and links these features to the roles of and complex communication between multiple cellular contributors in the muscle niche, highlighting the importance of a targeted approach to therapeutic intervention.

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Electron probe X-ray microanalysis of human skeletal muscle involved in rheumatoid arthritis

Cited by 21 publications

References 13 publications

Genuine myotubular myopathy

Genuine myotubular myopathy

Elemental composition of muscle at rest and potassium levels in muscle, plasma and sweat of horses exercising at 20°C and 35°C

Rheumatoid cachexia: the underappreciated role of myoblast, macrophage and fibroblast interplay in the skeletal muscle niche

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