Electron spin resonance investigations of human retinal pigment epithelium melanosomes from young and old donors

Bilińska, Barbara; Pilawa, Barbara; Zawada, Zygmunt H.; Wylęgała, Edward; Wilczok, Tadeusz; Донцов, А. Е.; Сакина, Н. Л.; Оstrovsky, М. А.; Ilyasova, V.B

doi:10.1016/s1386-1425(01)00695-3

Cited by 20 publications

(32 citation statements)

References 13 publications

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“…The isolated fractions were dried up as described above. Pigment identification was based on assessment of their solubility, spectral and paramagnetic properties (Ostrovsky, Dontsov, 1985;Bilińska, et al, 2002.). Measurements of the spectra of the electron paramagnetic resonance (EPR) were performed in the EPR spectrometer Bruker EMX (Germany).…”

Section: Methodsmentioning

confidence: 99%

Melanin properties at the different stages towards life cycle of the fly Hermetia illucens

Ушакова¹,

Донцов²,

Сакина³

et al. 2017

Ukr J Ecol

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Eumelanin type pigments are synthesized at all the stages of the life cycle of the fly Hermetia illucens: in the larvae, pre-pupae, pupae and adult flies (dead flies). The greatest content of melanin was recorded in the cuticles. Melanin was present not only in the cuticle, hence it remained in the cuticle after the emergence of the adult fly. It was also found in the insect body in a complex with lipids. In pupae, it is mostly lauric acid that was associated with melanin. Its proportion in the melanin-chitosan complex was 80%. The isolated melanin-chitosan complex of adult flies showed a wide range of antibacterial activity, inhibiting the growth of 21 out of the 25 of the test cultures. The melanin-chitosan complex of empty pupal membranes and alcohol suspension of pupal melanin inhibited twice as smaller number of test cultures and the above activity was absolutely in the pupal chitosan. The largest zone of growth inhibition was recorded with respect to Aspergillus niger, Candida albicans, salmonella, and Staphylococcus aureus. An alcohol suspension of pupal melanin inhibited the growth of 10 test cultures. In this case the greatest activity was shown in relation to Mycobacterium B5 and Acinetobacter sp. 1182.Key words: melanin; melanin-chitosan complex; Hermetia illucens; antibacterial activityThe fly Hermetia illucens is a typical member of Stratiomyidae, Diptera, Insecta (Linnaeus, 1758) is characterized by complete metamorphosis, including the main developmental stages as the egg, larva, pre-pupae, pupae, adult individual. It is only larvae that feed. Larvae are able to recycle different organic waste, using them as foraging substrates. This property determines the increased interest in these insects and the peculiarities of their biochemical composition in connection with the prospects of using insect meal in feed and food (. Although the chemical composition of the insect larvae varies with food substrate the fat content averages 30% (Diener, et al., 2011;St-Hilaire, et al., 2007;Gobbi, et al., 2013;Kalová, Borkovcová, 2013). Adult flies are equally fat. Our data demonstrate that fat content in dead flies accounts for 27%, and that in the larvae raised on wheat grains, 38%, and in prepupae, 32%. The large amount of fat in the body of the fly necessitates its antioxidant protection, which may be provided by melanin pigments. Melanins are nitrogen-containing compounds of irregular structure on the basis of phenol, which are the main pigment of insects. They create the dark-brown or brown coloration of insects, being created by the insects themselves, and become part of the cuticle, are present in the hemolymph, adipose body, and the intestinal tract (Nicolaus, 1968;Prota, 1992). The pigments have antioxidant, radio-and photo-protector, electron and ion-exchange properties (Dontsov, 2014;Ostrovsky, Dontsov, 1985). Natural melanin pigments show antibacterial and immune-modulation activity. They can form diverse complexes with proteins, various pharmacological agents, for instance, with antibiotics, which i...

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Section: Methodsmentioning

confidence: 99%

Melanin properties at the different stages towards life cycle of the fly Hermetia illucens

Ушакова¹,

Донцов²,

Сакина³

et al. 2017

Ukr J Ecol

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“…o-Semiquinone free radicals structure is presented in Figure 10 (Kozdrowska, 2006;Pasenkiewicz-Gierula, 1990;Sarna, 1981). Our earlier EPR studies of human retinal pigment epithelium melanosomes from young and old donors pointed out that the amount of free radicals depend on the age and method of irradiation (Bilińska et al, 2002). It seems that free radical reactions in melanin of eye influences on therapeutic effect of laser irradiation.…”

Section: Advances In Ophthalmology 16mentioning

confidence: 99%

“…Lasers in ophthalmology is used to the damage the eye structures via conducting of the energy from melanin biopolymer to them (Gilmour, 2002). Mainly eumelanins with the chemical structure presented in Figure 9 exist in eye (Bilińska et al, 2002;PasenkiewiczGierula, 1990;Sarna, 1981;Zdybel, 2008). (Wakamatsu & Ito, 2002).…”

Section: Advances In Ophthalmology 16mentioning

confidence: 99%

Lasers in Ophthalmology

Zdybel¹,

Pilawa²,

Krzeszewska-Zaręba³

2012

Advances in Ophthalmology

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“…Indeed‚ a recent EPR study has shown that the amount of RPE melanin decreases by a factor of 2.5 between the first and ninth decade of life (Sarna et al‚ 2002). In an independent study‚ the melanin content of RPE melanosomes from human donors of different age was analyzed by EPR after drying the pigment granules (Bilinska et al‚ 2002). Surprisingly‚ the content of melanin‚ when normalized to a single pigment granule‚ was found independent of age.…”

Section: Ocular Melaninmentioning

confidence: 99%

“…Surprisingly‚ the content of melanin‚ when normalized to a single pigment granule‚ was found independent of age. On the other hand‚ RPE melanosomes from younger individuals could contain significantly higher percentage of strongly bound water‚ that those from older individuals (Bilinska et al‚ 2002). RPE melanosomes from older human donors exhibit increased photoreactivity: they induce faster oxygen photouptake and accumulation of superoxide anion spin adducts (Rozanowska et al‚ 2002).…”

Section: Ocular Melaninmentioning

confidence: 99%

Free Radicals and Medicine

Swartz

Mason

Hogg

et al. 2005

Biomedical EPR, Part A: Free Radicals, Metals, Medicine, and Physiology

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Abstract:EPR has been employed in attempts to understand the basis of specific pathophysiologies in which free radicals have a postulated role. Examples described include pulmonary free radical damage‚ free radicals and sickle cell disease‚ free radicals in amyotrophic lateral sclerosis‚ melanin and free radicals and the potential role of oxidative stress in the induction of cancer. The final section of the chapter describes the use of NMR as the spectroscopic measure of spin-trapped radicals‚ after they have reacted further to form diamagnetic species.

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Electron spin resonance investigations of human retinal pigment epithelium melanosomes from young and old donors

Cited by 20 publications

References 13 publications

Melanin properties at the different stages towards life cycle of the fly Hermetia illucens

Melanin properties at the different stages towards life cycle of the fly Hermetia illucens

Lasers in Ophthalmology

Free Radicals and Medicine

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