Electron Spin Resonance Spectroscopy Does Not Reveal Hydroxyl Radical Production in Activated Natural Killer Lymphocytes

Gibboney, James J.; Haak, Richard A.; Kleinhans, F.W.; Brahmi, Zacharie

doi:10.1002/jlb.44.6.545

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…Hydroxyl radical scavengers inhibit NK cell-mediated lysis of tumor targets (7,9). However, by electron spin reso-nance spectroscopy, hydroxyl radical production was not demonstrated in activated NK cells (9). While our data demonstrate that catechin profoundly inhibits lymphocyte-mediated fungistasis, it remains to be determined whether the mechanism is via the scavenging of hydroxyl radicals.…”

Section: Discussionmentioning

confidence: 59%

“…The potent oxidant hydroxyl radical has been implicated in phagocyte microbicidal activity (29), but it remains controversial as to whether the hydroxyl radical is generated by lymphocytes. Hydroxyl radical scavengers inhibit NK cell-mediated lysis of tumor targets (7,9). However, by electron spin reso-nance spectroscopy, hydroxyl radical production was not demonstrated in activated NK cells (9).…”

Section: Discussionmentioning

confidence: 99%

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Mechanisms of inhibition of Cryptococcus neoformans by human lymphocytes

et al. 1995

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Recently, our laboratory and others have demonstrated that human peripheral blood T and NK lymphocytes directly inhibit the growth of Cryptococcus neoformans. In this study, we further define the conditions under which lymphocyte-mediated fungistasis against C. neoformans occurs and examine whether mechanisms implicated in lymphocyte-mediated activities against other target cells are also involved in anticryptococcal activity. The addition of whole or broken heat-killed C. neoformans modestly inhibited lymphocyte-mediated fungistasis, whereas other particulates had no effect. The hydroxyl radical scavenger catechin, but not diethyl urea or propyl gallate, profoundly inhibited fungistasis. Salicylic acid inhibited fungistasis in a dose-dependent fashion. However, two other cyclooxygenase inhibitors, piroxicam and indomethacin, had no effect, suggesting that the mechanism of inhibition by salicylic acid was cyclooxygenase independent. Reagent prostaglandin E 2 , at concentrations shown by others to inhibit NK cell-mediated bactericidal and tumorlytic activities, had no effect on lymphocyte-mediated fungistasis. The addition of selected monoclonal antibodies or ligands reactive with receptors on human lymphocytes had no significant effect on lymphocyte-mediated fungistasis. Acapsular, small-capsuled, and large-capsuled C. neoformans organisms were inhibited by lymphocytes to an approximately equal extent. These data demonstrate that lymphocyte-mediated activity against C. neoformans proceeds regardless of the presence of capsule and by mechanisms at least in part dissimilar from those seen with other target cells.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Mechanisms of inhibition of Cryptococcus neoformans by human lymphocytes

et al. 1995

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“…There is a lot of data regarding the production of reactive oxygen species by phagocytes, so our study focused on nonclassical ROS-producing cells. For example, NK cells require reactive oxygen species for the lysis of pathogens [ 15 , 16 ]. T cells also produce reactive oxygen species during oxidative metabolism, which take part in regulating T cell functions [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Cucurbiturils on the Production of Reactive Oxygen Species by T- and B-Lymphocytes, Platelets and Red Blood Cells

Aktanova

Boeva

Barkovskaya

et al. 2023

IJMS

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Reactive oxygen species (ROS) are highly reactive chemical molecules containing oxygen. ROS play an important role in signaling and cell homeostasis at low and moderate concentrations. ROS could be a cause of damage to proteins, nucleic acids, lipids, membranes and organelles at high concentrations. There are a lot of cells that can produce ROS to maintain functional activity. It is known that metal nanoparticles can increase production of ROS in cells. However, the effect of cucurbiturils on ROS production is still unknown. In our study, we evaluated production of ROS by the immune (T-, B-lymphocytes, NK-cells) and non-immune cells (red blood cells, platelets), as well as tumor cells line (1301, K562) after treatment with cucurbiturils in vitro. Assessment of reactive oxide species (ROS) were provided by using dihydrorhodamine 123 (DHR 123). Fluorescence intensity and percentage DHR123 were measured by flow cytometry. Platelets, erythrocytes and activated T-helpers were changed the level of ROS production in response to stimulation with cucurbiturils. It was found that the percentage of these ROS-producing cells was reduced by cucurbiturils. Thus, cucurbiturils may affect the production of ROS by cells, but further research is needed in this area.

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Enzymatic Sources of Free Radicals

Volodyaev,

Vladimirov

2023

Ultra-Weak Photon Emission From Biological Systems

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Electron Spin Resonance Spectroscopy Does Not Reveal Hydroxyl Radical Production in Activated Natural Killer Lymphocytes

Cited by 6 publications

References 29 publications

Mechanisms of inhibition of Cryptococcus neoformans by human lymphocytes

Mechanisms of inhibition of Cryptococcus neoformans by human lymphocytes

Influence of Cucurbiturils on the Production of Reactive Oxygen Species by T- and B-Lymphocytes, Platelets and Red Blood Cells

Enzymatic Sources of Free Radicals

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