Electronic Olfactory Sensor Based on <i>A. mellifera</i> Odorant‐Binding Protein 14 on a Reduced Graphene Oxide Field‐Effect Transistor

Larisika, Melanie; Kotlowski, Caroline; Steininger, Christoph; Mastrogiacomo, Rosa; Pelosi, Paolo; Schütz, Stefan; Peteu, Serban F.; Kleber, Christoph; Reiner‐Rozman, Ciril; Nowak, Christoph; Knoll, Wolfgang

doi:10.1002/anie.201505712

Cited by 82 publications

(55 citation statements)

References 31 publications

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“…The detection of biorecognition events with FET biosensors also allows for the investigation of the thermodynamics of the process. [9,23,63] Different functions could be used to fit the dose curve in Fig. 2b, viz.…”

Section: Resultsmentioning

confidence: 99%

EGOFET Peptide Aptasensor for Label‐Free Detection of Inflammatory Cytokines in Complex Fluids

et al. 2017

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Organic electronic transistors are rapidly emerging as ultra-high sensitive label-free biosensors suited for point of care or in-field deployed applications. Most organic biosensors reported to date are based on immunorecognition between the relevant biomarkers and the immobilized antibodies, whose use is hindered by large dimensions, poor control of sequence and relative instability. Here, we report an Electrolyte Gated Organic Field Effect Transistor (EGOFET) biosensor where the recognition units are surface immobilized peptide aptamers (Affimerä proteins) instead of antibodies. We demonstrate our peptide aptasensor for the detection of the pro-inflammatory cytokine Tumor Necrosis Factor alpha (TNFa) with a 1pM limit of detection. Ultra-low sensitivity is met even in complex solutions such as cell culture media containing 10 % serum, demonstrating the remarkable ligand specificity of our device. The device performances, together with the simple one-step immobilization strategy of the recognition moieties and the low operational voltages, all prompt EGOFET peptide aptasensors as candidates for early diagnostics and monitoring at the point-of-care.

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Section: Resultsmentioning

confidence: 99%

EGOFET Peptide Aptasensor for Label‐Free Detection of Inflammatory Cytokines in Complex Fluids

et al. 2017

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“…By incorporating functional groups with specific chemical or biological recognition ability, graphene‐based biosensors can be utilized to detect multiple biomolecules, such as DNA, protein, glucose, dopamine, etc. For example, Knoll and co‐workers demonstrated an olfactory biosensor based on a reduced graphene oxide (rGO) field‐effect transistor (FET) functionalized by the odorant‐binding protein 14 for the in situ and real‐time monitoring of a broad spectrum of odorants in aqueous solutions . Haghiri‐Gosnet and co‐workers recently reported an electrochemical biosensor based on graphene nanomesh with 260 nm wide nanoholes for the ultrasensitive detection of DNA .…”

Section: Introductionmentioning

confidence: 99%

Ultrafine Graphene Nanomesh with Large On/Off Ratio for High‐Performance Flexible Biosensors

Yang

Zou

et al. 2016

Adv Funct Materials

132

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Graphene is an attractive material for flexible electronics and biosensors, yet its zero bandgap nature has limited the on/off ratio of field-effect transistors (FETs) and the sensitivity of biosensors based on graphene. Graphene nanomesh (GNM), a continuous 2D graphene nanostructure with a high density of holes punched in the basal plane, has been created to introduce lateral confinement and enable improved on/off ratio. However, the GNMs produced to date typically have a relatively large dimension (constriction neck width >5 nm) and low on/off ratio (≈100) limited by the resolution of the lithography process used. Here, the exploration of a directly grown mesoporous silica template is reported for the preparation of ultrafine GNMs with considerably narrower neck width (<3 nm) and strong quantum confinement to enable flexible FETs with greatly improved on/off ratio (up to 1000). With excellent electronic properties and high surface area for the functionalization of specific receptors, it is further shown that the GNM FETs can be readily used to construct highly sensitive biosensors for selective detection of human epidermal growth factor receptor 2, which is further demonstrated for real-time detection of breast cancer cells overexpressed with receptor 2 down to single-cell level. The studies provide a simple and scalable method to GNMs with potential applications for flexible nanoelectronics and biosensors.

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“…Except for studies using spheroidal HEK cells [97], most research studies of cell-based odorant sensors presented here used detergent-solubilized odorous substances and not gaseous odorants as target samples. For the direct detection of airborne molecules from the atmosphere, there are few recent reports of the use of odorant-binding proteins [164,165]. In addition, to form the air-liquid interface near the cell surface, some studies suggest the use of collagen to encapsulate HEK cells and corneal epithelial cells as the host cells for expressing olfactory receptors [166,167].…”

Section: Discussionmentioning

confidence: 99%

Membrane protein-based biosensors

Misawa

Osaki

Takeuchi

2018

J. R. Soc. Interface.

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This review highlights recent development of biosensors that use the functions of membrane proteins. Membrane proteins are essential components of biological membranes and have a central role in detection of various environmental stimuli such as olfaction and gustation. A number of studies have attempted for development of biosensors using the sensing property of these membrane proteins. Their specificity to target molecules is particularly attractive as it is significantly superior to that of traditional human-made sensors. In this review, we classified the membrane protein-based biosensors into two platforms: the lipid bilayer-based platform and the cell-based platform. On lipid bilayer platforms, the membrane proteins are embedded in a lipid bilayer that bridges between the protein and a sensor device. On cell-based platforms, the membrane proteins are expressed in a cultured cell, which is then integrated in a sensor device. For both platforms we introduce the fundamental information and the recent progress in the development of the biosensors, and remark on the outlook for practical biosensing applications.

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Electronic Olfactory Sensor Based on A. mellifera Odorant‐Binding Protein 14 on a Reduced Graphene Oxide Field‐Effect Transistor

Cited by 82 publications

References 31 publications

EGOFET Peptide Aptasensor for Label‐Free Detection of Inflammatory Cytokines in Complex Fluids

EGOFET Peptide Aptasensor for Label‐Free Detection of Inflammatory Cytokines in Complex Fluids

Ultrafine Graphene Nanomesh with Large On/Off Ratio for High‐Performance Flexible Biosensors

Membrane protein-based biosensors

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