Electronically addressable nanomechanical switching of i-motif DNA origami assembled on basal plane HOPG

Abstract

Here, a pH-induced nanomechanical switching of i-motif structures incorporated into DNA origami bound onto cysteamine-modified basal plane HOPG was electronically addressed, demonstrating for the first time the electrochemical read-out of the nanomechanics of DNA origami. This paves the way for construction of electrode-integrated bioelectronic nanodevices exploiting DNA origami patterns on conductive supports.

Distortion of DNA Origami on Graphene Imaged with Advanced TEM Techniques

“…We find that DNA origami is also distorted when deposited onto highly oriented pyrolytic graphite (HOPG), which has a surface very similar to that of graphene. Although this may seem trivial, there is no consensus on whether and how DNA origami interacts with the HOPG surface . The different reported results may be due to different experimental conditions including buffer, pH, salt concentrations, or biased sampling of the imaging area.…”

Chemistries for DNA Nanotechnology

“…531 For instance, HOPG was treated with cysteamine which afforded a positively charged layer on the surface and enabled immobilization of DNA origami. 532 Aminomodified silicon surfaces were also used for immobilization of graphene oxide whose carboxylic acid groups reacted in amidebond forming reactions with the surface-amines by annealing at 180 °C. 533 This enabled the efficient immobilization of DNA origami on graphene oxide and on nitrogen-doped graphene oxide by Mg 2+ -bridging, whereas binding to reduced graphene oxide was less efficient.…”

“…Highly oriented pyrolytic graphite (HOPG) can also be rendered charged through reaction with carboxylate or amino-terminated alkane thiols . For instance, HOPG was treated with cysteamine which afforded a positively charged layer on the surface and enabled immobilization of DNA origami . Amino-modified silicon surfaces were also used for immobilization of graphene oxide whose carboxylic acid groups reacted in amide-bond forming reactions with the surface-amines by annealing at 180 °C .…”

Electrocatalytic Oxidation of Water by OH−‐ and H2O‐Capped IrOx Nanoparticles Electrophoretically Deposited on Graphite and Basal Plane HOPG: Effect of the Substrate Electrode**

“…These electrode materials have distinctly different surface properties with respect to hydrophilicity/hydrophobicity, sur-face reactivity [50][51][52][53][54] and surface structural roughness. [55] While the atomically flat surface of HOPG is extremely useful as a substrate for AFM studies, [52,56,57] its reactivity is also very attractive for electrochemical applications. [51][52][53][54] Cheap and easily renewable spectroscopic Gr, widely used in electroanalysis of small molecules and proteins due to its high-surface area enabling a higher surface coverage with adsorbates, [14,[58][59][60][61][62] has been already used for fabrication of IrO x nanocomposite electrodes.…”