Electrophoretic Analysis of Isoforms of Glyoxalase II in Clinical Blood Samples

Abstract

Summary:A non-denaturing polyacrylamide gel electrophoresis procedure is described for the analysis of isoforms of human red blood cell glyoxalase II (EC 3.1.2.6). A cathodic, continuous buffer electrophoresis system was developed, using a 60 g/1 polyacrylamide gel with 25 g/1 N^-methylene-bis-acrylamide in 50 mmol/1 sodium succinate buffer, pH 5.5 and 1 °C. Staining for glyoxalase II activity involved

Determination of rat blood S-d-lactoylglutathione by a column-switching high-performance liquid chromatography with precolumn fluorescence derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole

“…The evaluation method for the glyoxalase system has frequently been performed to measure the gene expression or the amount of the two enzymes (Gly I and II) [19,20] and to monitor the concentration of SLG [21] for the purpose of estimating the activities of these enzymes. SpeciWcally, the activity of Gly I has been evaluated by monitoring the increase in absorbance at 240 nm due to the formation of SLG, and the activity of Gly II by monitoring the decrease of SLG in the presence of tissue homogenates.…”