Electrophysiological basis of human Fallopian tubal fluid formation

Downing, Sandra J.; Maguiness, S.D.; Watson, Alan J.; Leese, Henry J.

doi:10.1530/jrf.0.1110029

Cited by 18 publications

(8 citation statements)

References 20 publications

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“…First, the epithelial cells have to be isolated from sexually immature mouse uteri in order to enable proliferation in culture, which may not have the same gene expression profile as in mature mice. Secondly, some protein expression or function may be lost during the culture as observed in many cell cultures including human fallopian tubule cells (Downing et al 1997). Most importantly, the culture condition cannot reproduce the cyclic characteristics normally observed in vivo, considering the fluctuating levels of ovarian hormones.…”

Section: Discussionmentioning

confidence: 99%

Regulatory mechanism underlying cyclic changes in mouse uterine bicarbonate secretion: role of estrogen

Chen²,

Wong³

et al. 2010

Reproduction

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Our previous study has demonstrated that bicarbonate in the uterine fluid plays an indispensable role in sperm capacitation. However, the cellular mechanisms underlying the formation of bicarbonate-rich uterine fluid and the regulatory mechanism remained largely unknown. In this study, the expression profiles of bicarbonate transport/production proteins, the cystic fibrosis transmembrane conductance regulator (CFTR), SLC26A6, carbonic anhydrase 2 (CAR2, CA2) and CAR12 (CA12), throughout the estrous cycle, were examined in the mouse uterus by western blot. The results showed that the maximum expression levels of the proteins examined were observed at estrus. Luminal surface pH measurements showed that the resting uterine surface pH at estrus was significantly higher than that at diestrus, which could be reduced significantly by CFTR blocker, diphenylamine-2,2 0 -dicarboxylic acid, SLC26A6 inhibitor, 4 0 ,4 0 -diisothiocyanostilbene-2 0 ,2 0 -disulfonic acid, and CA inhibitor, acetazolamide. In ovariectomized mice and primary culture of endometrial epithelial cells, estrogen could upregulate CFTR, SLC26A6, CAR2, and CAR12 expression with a corresponding increase in the bicarbonate-dependent short-circuit current (I sc ) and endometrial surface pH. The present results have demonstrated dynamic changes in uterine bicarbonate secretion and expression of the proteins involved in bicarbonate secretion during the estrous cycle and suggested a novel role of estrogen in regulating uterine bicarbonate transport, which may be important for successful reproduction.Reproduction (2010) 140 903-910

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Section: Discussionmentioning

confidence: 99%

Regulatory mechanism underlying cyclic changes in mouse uterine bicarbonate secretion: role of estrogen

Chen²,

Wong³

et al. 2010

Reproduction

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“…The spermatozoa then free themselves from the oviductal reservoir and, ultimately, hyperactivation causes a whiplashlike flagella beating, which helps penetration of the egg vestments. ATP is known to act as a signaling molecule on ciliated airway and oviduct epithelium, increasing cilary beat frequency via a calcium-dependent pathway (Barrera et al, 2004;Stutts et al, 1992), and ATP added apically or basally to oviduct epithelial cells can modulate the formation of oviductal fluid (Downing et al, 1997). As well as providing energy for the spermatozoa to swim to the site of fertilization in the oviduct, ATP may, therefore, be acting as a signaling molecule, increasing ciliary beat frequency and helping move the spermatozoa through the oviduct or release them from the oviductal reservoir.…”

Section: Discussionmentioning

confidence: 99%

Adenosine Triphosphate Production by Bovine Spermatozoa and Its Relationship to Semen Fertilizing Ability

Garrett

Revell

Leese

2008

Journal of Andrology

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This article's objectives are to investigate the relationship between adenosine triphosphate (ATP) production (oxidative phosphorylation and glycolysis) and fertility of bovine spermatozoa, determine the proportion of oxygen consumption devoted to proton leak and that due to nonmitochondrial processes, and discover whether freeze/thawing affects sperm oxygen consumption. Oxygen consumption of bovine spermatozoa was measured using a standard Clark electrode and, for the first time, in an Oxygen Biosensor System (OBS). Total ATP formation by bovine spermatozoa was calculated from the oxygen consumption and lactate production (glycolysis) by the same spermatozoa sample. ATP production varied from 1.99 to 8.09 mmol ATP per 10 8 spermatozoa per hour; glycolysis accounted for 16% to 38% of ATP. Nonmitochondrial oxygen consumption could not be detected in bovine spermatozoa using these methods. A significant proportion (16%-43%) of oxygen consumption was insensitive to oligomycin and was due to ''proton leak.'' There was no significant difference between oxygen consumption of frozen/thawed and fresh spermatozoa for 2 of the 3 bulls tested. However, oxygen consumption of frozen/thawed spermatozoa was significantly higher (P , .05) than fresh spermatozoa for the third bull. When ZO 2 of frozen/thawed spermatozoa from 20 bulls was compared with their 49 day nonreturn rates (NRRs), oxygen consumption was correlated positively with NRR (ie, fresh spermatozoa with a higher ZO 2 were more fertile). Moreover, total ATP production correlated with NNR better than ZO 2 . Bulls with a lower NRR produce spermatozoa that are susceptible to damage during the freeze/thawing process, causing an increase in ZO 2 , possibly due to mitochondrial membrane damage resulting in more energy being expended in maintaining the proton gradient, or capacitation-like changes causing hyperactivation. Oxygen consumption measured in the OBS may be useful in assessing bovine sperm fertility.Key words: Fertility, semen analysis, sperm, oxygen consumption. J Androl 2008;29:449-458A rtificial insemination (AI) accounts for approximately 80% of breedings in the United Kingdom dairy herd. However, the efficiency of AI is highly variable, with an average of 1.7 doses of semen being required per cow to establish a pregnancy. There is a continuing need for more reliable markers of sperm fertilizing ability. A simple, relatively inexpensive method that correlates with nonreturn rate (NRR; sperm fertility measured by the percentage of cows that do not require reinsemination) could reduce the number of low-fertility bulls that enter the costly proofing program of a stud. These could then be replaced with bulls of higher fertility. The test could also be used routinely to assess the fertility of semen before and/or after it is processed for AI. Current methods to evaluate semen quality include tests of motility, morphology, membrane integrity and membrane strength (osmotic resistance test), chromatin structure, and gamete interactions (Rodriguez-Martinez,...

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“…Where blockers of histamine receptors or ion channels were used, the cells were incubated in the presence of the blocker on both apical and basal bathing medium for 20 min prior to addition of histamine. The effect of the blockers of ion channels on PD and I scc have been shown previously [6].…”

Section: Epithelial Cell Culturementioning

confidence: 85%

“…Immunocytochemical analyses demonstrated that a pure population of epithelial cells were isolated and that the cells formed extensive desmosome junctions in culture. We have subsequently shown that the movement of chloride ions is an important factor in the generation of the transepithelial potential difference across these cultured human oviductal epithelial cells and that extracellular ATP is a potent modulator of electrophysiological activity [6].…”

Section: Introductionmentioning

confidence: 96%

Effect of Inflammatory Mediators on the Electrophysiology of the Human Oviduct1

Downing

Chambers

Maguiness

et al. 1999

Biology of Reproduction

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The effects of histamine and other inflammatory mediators on the electrophysiology and intracellular free calcium ([Ca(2+)](i)) of human oviductal epithelial cells, grown as a polarized layer in primary culture, were studied. Transepithelial potential difference (PD) and short-circuit current (I(scc)) were recorded using a modified Ussing chamber. Resistance (R) was calculated from the measurements of PD and I(scc). Basally applied histamine produced transient increases in PD and I(scc) with a small decrease in R. The histamine effect was reduced by triprolidine (H(1) receptor antagonist) but was unaffected by H(2) (ranitidine) or H(3) (thioperamide) receptor antagonists. Blockers of Na(+), K(+), or Na(+)/K(+)/2Cl(-) channels did not affect histamine action. Blockers of Cl(-)/HCO(3)(-) channels or Ca(2+) channels reduced the histamine effect. Platelet activating factor (PAF), applied apically, increased PD and I(scc). Histamine produced a transient increase in fluorescence of Fura 2-AM dye, indicating an increase in [Ca(2+)](i). Triprolidine pretreatment inhibited histamine-stimulated [Ca(2+)](i) increase. Cimetidine, (H(2) receptor antagonist), ranitidine, or thioperamide reduced the histamine effect. Histamine increased contractions of both circular and longitudinal smooth muscles in oviduct segments, an effect that was antagonized by triprolidine or thioperamide but not by ranitidine. Histamine's action on Ca(2+) and Cl(-) movements may adversely affect oviductal fluid production and decrease fertility. PAF's effects on Cl(-) movements may be important for normal embryo transport.

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Electrophysiological basis of human Fallopian tubal fluid formation

Cited by 18 publications

References 20 publications

Regulatory mechanism underlying cyclic changes in mouse uterine bicarbonate secretion: role of estrogen

Regulatory mechanism underlying cyclic changes in mouse uterine bicarbonate secretion: role of estrogen

Adenosine Triphosphate Production by Bovine Spermatozoa and Its Relationship to Semen Fertilizing Ability

Effect of Inflammatory Mediators on the Electrophysiology of the Human Oviduct1

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