Electrophysiological differences between nociceptive and non‐nociceptive dorsal root ganglion neurones in the rat <i>in vivo</i>

Fang, Xin; McMullan, Simon; Lawson, Sally N.; Djouhri, Laiche

doi:10.1113/jphysiol.2005.086199

Cited by 200 publications

(241 citation statements)

References 48 publications

(119 reference statements)

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“…In contrast, specific high-threshold cold neurons would result from the following code: low TRPM8 levels and high expression of I KD . This population appears to be functionally distinct from conventional C-type polymodal nociceptor neurons that exhibit broad spikes and lack inward rectification (Scroggs et al, 1994;Fang et al, 2005). The high-threshold cold receptors we describe probably underlie the common sensation of unpleasant cold discomfort (Mauderli et al, 2003), accompanying, for example, sudden body immersion in cold water or exposure to very cold air.…”

Section: Discussionmentioning

confidence: 93%

Variable Threshold of Trigeminal Cold-Thermosensitive Neurons Is Determined by a Balance between TRPM8 and Kv1 Potassium Channels

Madrid¹,

Peña²,

et al. 2009

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Molecular determinants of threshold differences among cold thermoreceptors are unknown. Here we show that such differences correlate with the relative expression of I KD , a current dependent on Shaker-like Kv1 channels that acts as an excitability brake, and I TRPM8 , a cold-activated excitatory current. Neurons responding to small temperature changes have high functional expression of TRPM8 (transient receptor potential cation channel, subfamily M, member 8) and low expression of I KD . In contrast, neurons activated by lower temperatures have a lower expression of TRPM8 and a prominent I KD . Otherwise, both subpopulations have nearly identical membrane and firing properties, suggesting that they belong to the same neuronal pool. Blockade of I KD shifts the threshold of cold-sensitive neurons to higher temperatures and augments cold-evoked nocifensive responses in mice. Similar behavioral effects of I KD blockade were observed in TRPA1 Ϫ/Ϫ mice. Moreover, only a small percentage of trigeminal cold-sensitive neurons were activated by TRPA1 agonists, suggesting that TRPA1 does not play a major role in the detection of low temperatures by uninjured somatic cold-specific thermosensory neurons under physiological conditions. Collectively, these findings suggest that innocuous cooling sensations and cold discomfort are signaled by specific low-and high-threshold cold thermoreceptor neurons, differing primarily in their relative expression of two ion channels having antagonistic effects on neuronal excitability. Thus, although TRPM8 appears to function as a critical cold sensor in the majority of peripheral sensory neurons, the expression of Kv1 channels in the same terminals seem to play an important role in the peripheral gating of cold-evoked discomfort and pain.

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Section: Discussionmentioning

confidence: 93%

Variable Threshold of Trigeminal Cold-Thermosensitive Neurons Is Determined by a Balance between TRPM8 and Kv1 Potassium Channels

Madrid¹,

Peña²,

et al. 2009

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“…Medium-size cells with ADP display functional properties of nociceptors, with IB 4 -positive immunoreactivity in both control and diabetic groups but enhanced responses to capsaicin only in diabetic rats Previous in vivo recordings with sharp electrodes have shown that many medium-size DRG cells belong to slowly conducting A␦ sensory fibers, most of which are high-threshold mechanoreceptors capable of sensing painful stimuli (Harper and Lawson, 1985;Lee et al, 1986;Gerke and Plenderleith, 2001;Fang et al, 2005). Although acute enzymatic dissociation allows patch clamping and studies of ion channels, functional identification of dissociated DRG cells based on conduction velocity is not feasible.…”

Section: Diabetes Alters Biophysical Properties Of Voltage-dependent mentioning

confidence: 99%

Cell-Specific Alterations of T-Type Calcium Current in Painful Diabetic Neuropathy Enhance Excitability of Sensory Neurons

Jagodic¹,

Pathirathna²,

Nelson³

et al. 2007

J. Neurosci.

240

218

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“…У роботах in vivo детально описано електрофізіологічні показники нейронів ГТН відповідно до типу модальності сигналу та швидкості проведення нервового імпульсу [3,4]. Цей ганглій, як усі сенсорні ганглії, складається з гетерогенних за морфологічними та елек-трофізіологічними показниками нейронів [5,6]. Вивчення властивостей нейронів сенсорних волокон представляє великий інтерес, бо у більшості випадків порушення периферичних волокон призводять до змін в іонній провідності мембрани сом та нейритів, внутрішньоклітинних механізмах, синтезі білків та пептидів [7,8].…”

Section: вступunclassified

“…В електрофізіологічних дослідженнях показано, що для ноцицепторів сенсорних гангліїв характерна генерація тривалих потенціалів дії (ПД) з «горбом» на фазі реполяризації [3,6]. Окрім електричних характеристик ноцицептори вирізняються такими фармакологічними показниками, як чутливість до капсаїцину, резистивність до тетродотоксину тощо [10 -12].…”

Section: вступunclassified

Excitability Properties of Trigeminal Ganglion Neurons

Tel’ka¹,

Rikhalsky²,

Veselovsky³

2016

Fiziol Zh

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Electrophysiological differences between nociceptive and non‐nociceptive dorsal root ganglion neurones in the rat in vivo

Cited by 200 publications

References 48 publications

Variable Threshold of Trigeminal Cold-Thermosensitive Neurons Is Determined by a Balance between TRPM8 and Kv1 Potassium Channels

Variable Threshold of Trigeminal Cold-Thermosensitive Neurons Is Determined by a Balance between TRPM8 and Kv1 Potassium Channels

Cell-Specific Alterations of T-Type Calcium Current in Painful Diabetic Neuropathy Enhance Excitability of Sensory Neurons

Excitability Properties of Trigeminal Ganglion Neurons

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