Electrophysiological identification of alpha- and beta-intercalated cells and their distribution along the rabbit distal nephron segments.

Muto, Shigeaki; Yasoshima, Koichi; Yoshitomi, Kuniaki; Imai, Masashi; Asano, Yoshihide

doi:10.1172/jci114913

Cited by 94 publications

(156 citation statements)

References 47 publications

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“…Our present approach is therefore noteworthy in that the stable puncture of the collecting duct cells of mammalian kidney can finally be accomplished. The value for Yb observed in the present study is as large as that reported by other investigators (Koeppen et al 1983; Schlatter and Schafer 1987; Sansom et al 1989;Muto et al 1990). We did not use differential phase contrast optics in the present study.…”

Section: Discussionsupporting

confidence: 62%

New Double-Barreled, Ion-Sensitive Microelectrodes for Measuring Intracellular Cl- Activities in Rabbit Renal Collecting Ducts.

Kondo¹,

Igarashi²,

Abe

et al. 1993

Tohoku J. Exp. Med.

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The use of the conventional ion-sensitive double-barreled microelectrodes has been unsuccessful for studying small epithelial cells such as those of the collecting duct because of the difficulty in obtaining the ideal electrodes for impaling cells. We developed a new type of ion-sensitive, double-barreled microelectrode of a size and tip configuration ideal for use in impaling small cells such as the principal cells (PCs) of the cortical collecting ducts, and remaining therein, for more than 30 min. The electrodes were pulled in two steps. The first includes the reciprocal twisting of two parallel glass capillaries, without pulling, to form a round, nontortuous fused glass capillary in the puller. In the second step, the round, fused straight capillaries are pulled so as to form the tip. This resulting procedure enabled us to impale the PCs without altering their cell membrane potentials. The basolateral membrane voltage (Vb) in PCs of the cortical collecting ducts was -69.1±1.6 mV (n=8) and intracellular C1-activity ([C1],) in PCs of the cortical collecting duct was 11.8±0.9 mmol/liter (n=5).Changing the concentration of C1-in the ambient solution showed that the basolateral cell membrane of the PCs was highly permeable to Cl-. This new electrode will help to obtain new information on intracellular ion handling, with the electrodes applied to the collecting duct cells. chloride transport ; ion exchanger; renal tubule ; electrophysiology ; aluminosilicate glassThe use of the ion-sensitive microelectrodes in studies of renal electrophysiology has provided considerable information on the mechanism of ion transport across the cell membrane of the renal tubule. Such studies have revealed, for instance, that the renal proximal tubular cells maintain a very low level of cellular Na+ by accumulating K+ into the cells, and that the cellular pH is kept slightly basic by several acid-extruding mechanisms such as the Na+/H+

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Section: Discussionsupporting

confidence: 62%

New Double-Barreled, Ion-Sensitive Microelectrodes for Measuring Intracellular Cl- Activities in Rabbit Renal Collecting Ducts.

Kondo¹,

Igarashi²,

Abe

et al. 1993

Tohoku J. Exp. Med.

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“…Because a moderate 01 -conductance exists in the basolateral membrane of the CNT cell (Yoshitomi et al 1988;Muto et al 1990), the observed hyperpolarization may reflect the reduction of intracellular Cl-concentration caused by inhibition of Clentry from the apical membrane of this type of cell. 5-8666 did not show any additive effect to TOM.…”

Section: Discussionmentioning

confidence: 99%

“…They include amiloride sensitive Na+ channel, Na+/H+ antiport, HC03-/C1-antiport and simple passive diffusion through paracellular pathway. Especially the HC03-/Cl-antiport which is mainly operated in the fl-IC cell may contribute to a large fraction of C1-flux in the CNT (Muto et al 1990). This is the reason why only small fraction of Cl-flux was inhibited by 5-8666 or TCM.…”

Section: Discussionmentioning

confidence: 99%

“…The rabbit CNTs were perfused in vitro as reported previously (Imai 1979 ;Shimizu et al 1988c ;Yoshitomi et al 1988 ;Muto et al 1990). The CNT was identified according to the criteria reported previously (Imai 1979).…”

Section: Methodsmentioning

confidence: 99%

“…To obtain direct evidence that both TOM and S-8666 act specifically on the CNT cell, we impaled the CNT cells with conventional microelectrodes filled with 0.5 M K01 while the tubules were perfused in vitro according to the method reported previously (Yoshitomi et al 1988;Muto et al 1990). The CNT cells were identified according to the following criteria ; (a) the magnitude of basolateral membrane voltage (VB) is greater than 60 mV, and (b) the positive VB deflection of greater than 20 mV is generated by abrupt increase in K+ concentration in the bath from 5 to 50 mM.…”

Section: Methodsmentioning

confidence: 99%

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Effect of S-8666 on Cl- Transport in the Rabbit Connecting Tubule Perfused in Vitro.

Shimizu

Nakamura

Imai³

1991

Tohoku J. Exp. Med.

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N-dimethylsulfamoyl)-2, 3-dihydrobenzofurancarboxylic acid] is a potent diuretic with uricosuric action. Although the major site of action of 5-8666 has been proven by the in vitro microperfusion study to be the thick ascending limb of Henle's loop, clearance studies in the rat suggested that this drug has an additional thiazide-like action. To provide direct evidence that 5-8666 acts also on distal nephron segments, we examined effect of 5-8666 on C1-flux across the rabbit connecting tubule perfused in vitro. The drug suppressed the lumen-to-bath 01 -flux by 96+41 (s.E.)pmol• mm-1 • min-1 (n =9) without affecting transmural voltage. To demonstrate that 5-8666 acts on the connecting tubule cell, the target of thiazide diuretics, we compared effects of S-8666 and trichlormethiazide on the basolateral membrane voltage of the connecting tubule cell. Both drugs added to the lumen caused a small but significant hyperpolarization of the basolateral membrane without affecting transmural voltage. We conclude that 5-8666 is a unique uricosuric diuretic having actions on both thick ascending limb of Henle's loop and connecting tubule.

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Chloride Transport

Edwards

2012

Comprehensive Physiology

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Chloride transport along the nephron is one of the key actions of the kidney that regulates extracellular volume and blood pressure. To maintain steady state, the kidney needs to reabsorb the vast majority of the filtered load of chloride. This is accomplished by the integrated function of sequential chloride transport activities along the nephron. The detailed mechanisms of transport in each segment generate unique patterns of interactions between chloride and numerous other individual components that are transported by the kidney. Consequently, chloride transport is inextricably intertwined with that of sodium, potassium, protons, calcium, and water. These interactions not only allow for exquisitely precise regulation but also determine the particular patterns in which the system can fail in disease states. © 2012 American Physiological Society. Compr Physiol 2:1061‐1092, 2012.

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Electrophysiological identification of alpha- and beta-intercalated cells and their distribution along the rabbit distal nephron segments.

Cited by 94 publications

References 47 publications

New Double-Barreled, Ion-Sensitive Microelectrodes for Measuring Intracellular Cl- Activities in Rabbit Renal Collecting Ducts.

New Double-Barreled, Ion-Sensitive Microelectrodes for Measuring Intracellular Cl- Activities in Rabbit Renal Collecting Ducts.

Effect of S-8666 on Cl- Transport in the Rabbit Connecting Tubule Perfused in Vitro.

Chloride Transport

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