Electrophysiological investigation of human embryonic stem cell derived neurospheres using a novel spike detection algorithm

Mayer, Margot; Arrizabalaga, Onetsine; Lieb, Florian; Ciba, Manuel; Ritter, S.; Thielemann, Christiane

doi:10.1016/j.bios.2017.09.034

Cited by 23 publications

(17 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, we examined nascent DNA replication rates in pairs of isogenic non-differentiated (nd) and differentiated (diff) samples. Human embryonic stem cells (hESCs) derived from the H9 cell line were differentiated into the neuronal lineage (ndiff) or spontaneously (sdiff), into the endoderm-like direction using pre-established procedures ( 29 , 30 ) (Figure 1A ). The differentiation status of the cells was confirmed by assessing the levels of: (a) the pluripotency marker Nanog ( 12 ), (b) p53 ( 12 ) and (c) the neurogenesis marker paired box 6 (PAX6) ( 31 ) ( Supplementary Figure S1A ).…”

Section: Resultsmentioning

confidence: 99%

Impact of the interplay between stemness features, p53 and pol iota on replication pathway choices

Ihle

Biber

Schroeder

et al. 2021

Nucleic Acids Research

View full text Add to dashboard Cite

Using human embryonic, adult and cancer stem cells/stem cell-like cells (SCs), we demonstrate that DNA replication speed differs in SCs and their differentiated counterparts. While SCs decelerate DNA replication, differentiated cells synthesize DNA faster and accumulate DNA damage. Notably, both replication phenotypes depend on p53 and polymerase iota (POLι). By exploring protein interactions and newly synthesized DNA, we show that SCs promote complex formation of p53 and POLι at replication sites. Intriguingly, in SCs the translocase ZRANB3 is recruited to POLι and required for slow-down of DNA replication. The known role of ZRANB3 in fork reversal suggests that the p53–POLι complex mediates slow but safe bypass of replication barriers in SCs. In differentiated cells, POLι localizes more transiently to sites of DNA synthesis and no longer interacts with p53 facilitating fast POLι-dependent DNA replication. In this alternative scenario, POLι associates with the p53 target p21, which antagonizes PCNA poly-ubiquitination and, thereby potentially disfavors the recruitment of translocases. Altogether, we provide evidence for diametrically opposed DNA replication phenotypes in SCs and their differentiated counterparts putting DNA replication-based strategies in the spotlight for the creation of therapeutic opportunities targeting SCs.

show abstract

Section: Resultsmentioning

confidence: 99%

Impact of the interplay between stemness features, p53 and pol iota on replication pathway choices

Ihle

Biber

Schroeder

et al. 2021

Nucleic Acids Research

View full text Add to dashboard Cite

show abstract

“…Spike detection was performed according to the stationary wavelet transform-based Teager energy operator (SWTTEO) algorithm that was presented previously 83 and revised for biological data 43 . The algorithm was implemented in a custom-made MATLAB (MathWorks) script.…”

Section: Methodsmentioning

confidence: 99%

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

Hyvärinen

Hyysalo

Kapucu

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Human pluripotent stem cell (hPSC)-derived neurons provide exciting opportunities for in vitro modeling of neurological diseases and for advancing drug development and neurotoxicological studies. However, generating electrophysiologically mature neuronal networks from hPSCs has been challenging. Here, we report the differentiation of functionally active hPSC-derived cortical networks on defined laminin-521 substrate. We apply microelectrode array (MEA) measurements to assess network events and compare the activity development of hPSC-derived networks to that of widely used rat embryonic cortical cultures. In both of these networks, activity developed through a similar sequence of stages and time frames; however, the hPSC-derived networks showed unique patterns of bursting activity. The hPSC-derived networks developed synchronous activity, which involved glutamatergic and GABAergic inputs, recapitulating the classical cortical activity also observed in rodent counterparts. Principal component analysis (PCA) based on spike rates, network synchronization and burst features revealed the segregation of hPSC-derived and rat network recordings into different clusters, reflecting the species-specific and maturation state differences between the two networks. Overall, hPSC-derived neural cultures produced with a defined protocol generate cortical type network activity, which validates their applicability as a human-specific model for pharmacological studies and modeling network dysfunctions.

show abstract

“…Microelectrodes are commonly used for applications involving neuronal cells, such as cochlear and retinal implants but also for neuro‐based in‐vitro assays to study neuronal network communication . Improving the interface between living cells and electronic devices is an important ongoing process, where the electrode material is one of the central components.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization of Graphene Microelectrodes for Biological Applications

et al. 2019

Self Cite

View full text Add to dashboard Cite

Graphene is a promising material both as a coating for existing neural electrodes as well as for transparent electrodes made exclusively from graphene. We studied graphene-based microelectrodes by investigating their recording and stimulation properties in order to evaluate their suitability for neuronal implants. In this work, we compare three different electrode material compositions. Microelectrode arrays (MEA) with an electrode size of about 700 mm 2 were prepared of gold, graphene on gold, and plain graphene on glass substrate. In order to reduce polymer contamination during graphene transfer, we employed a polymer-free transfer and lift-off process. Impedance studies revealed a value of 2.3 MW at 1 kHz for plain, and 0.88 MW for graphene on gold. Neuronal recording experiments showed a sufficient SNR for both graphene-based materials and a stable impedance, unaffected by surface degradation metal electrodes are known for. Stimulation measurements yielded a charge injection capacity of 0.15 mC/cm 2 using biphasic pulses of 1 ms and 1 mA transparent graphene electrodes. Cyclic voltammetry revealed a large voltage range of À1.4 V to + 1.6 V before water electrolysis occurs. Graphene-coated gold microelectrodes show enhanced recording properties, whereas plain graphene electrodes might be better suited for stimulation applications.

show abstract

Electrophysiological investigation of human embryonic stem cell derived neurospheres using a novel spike detection algorithm

Cited by 23 publications

References 35 publications

Impact of the interplay between stemness features, p53 and pol iota on replication pathway choices

Impact of the interplay between stemness features, p53 and pol iota on replication pathway choices

Functional characterization of human pluripotent stem cell-derived cortical networks differentiated on laminin-521 substrate: comparison to rat cortical cultures

Electrochemical Characterization of Graphene Microelectrodes for Biological Applications

Contact Info

Product

Resources

About