Electrophysiological Response of Two Moth Species, Helicoverpa armigera and Maruca vitrata to Host Plant (Cajanus cajan) Volatiles

Nebapure, Suresh M.

doi:10.1007/s40009-020-00898-z

Cited by 4 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For gravid female moths of G. molesta, our results showed that the EAG responses induced by ethyl butanoate at three concentrations exceeded that induced by the hexane control, and equaled the responses induced by the mixture. Similar results were found for the responses of Helicoverpa armigera (Hübner) and Maruca vitrata (Fabricius) to volatiles of Cajanus cajan L. (pigeon pea) when presented at four concentrations, with significant differences in the EAG responses of the moths (Nebapure 2020). Upwind flight frequencies of gravid females of G. molesta in response to each of the five tested esters presented individually exceeded the response to the hexane control, but no individual ester was better than the five-ester mixture.…”

Section: Discussionsupporting

confidence: 76%

Effects of pear fruit‐derived volatiles and their concentrations on the responses of oriental fruit moths

Hu,

Li,

Cao

et al. 2023

Entomological Research

View full text Add to dashboard Cite

Grapholita molesta (Busck) is an important pest of pear trees. Numerous esters accumulate consistently in mature pear fruits. However, little is known about the effects of single esters from pear fruits at different concentrations on the responses of male and female G. molesta. In this study, the responses of virgin males, virgin females, and gravid females to five esters (3‐methylbutyl acetate, ethyl hexanoate, ethyl butanoate, butyl acetate, and hexyl acetate) of pear fruits at three dosage levels (1, 5 and 10 μg/μL) were investigated using electroantennography (EAG), wind tunnel experiments, and field tests. Our results showed that ethyl butanoate (5 μg/μL) elicited higher EAG responses in virgin females and males than a mixture of all five volatiles, and elicited an equal EAG response to that for the mixture in gravid females, with all responses to ethyl butanoate and the mixture being higher than the response to hexane alone. For upwind flight, ethyl butanoate (5 μg/μL) excited equal moth flight–location behavior in virgin and gravid females to that for the mixture, which were both higher than the response to hexane. For close flight, ethyl hexanoate (5 μg/μL) and 3‐methylbutyl acetate (10 μg/μL) appeared to be the activating compounds, eliciting equal responses from virgin females to that of the mixture, and higher responses of gravid females to that of the mixture, both of which were higher than the responses to hexane. Upwind and close flight responses of virgin males were lower than those of virgin and gravid females. In field trials, we verified that traps baited with ethyl butanoate (5 μg/μL) captured more moths than the other esters. Therefore, from the esters and concentrations tested, we recommend ethyl butanoate (5 μg/μL) for potential use as an attractant for G. molesta in orchards.

show abstract

Section: Discussionsupporting

confidence: 76%

Effects of pear fruit‐derived volatiles and their concentrations on the responses of oriental fruit moths

Hu,

Li,

Cao

et al. 2023

Entomological Research

View full text Add to dashboard Cite

show abstract

“…The results obtained here were consistent with those of previous studies. According to a previous study, trans-βcaryophyllene played a role in prompting female H. armigera to lay eggs (Hartlieb & Rembold, 1996), and while this compound elicited responses in this species (Cribb et al, 2007;Nebapure, 2020), it did not induce a female response in the present experiment. This could be because the female moths used in this experiment were not mating, and trans-β-caryophyllene was mainly related to the oviposition of H. armigera.…”

Section: Discussionmentioning

confidence: 44%

“…Myrcene, linalool, and methyl salicylate play a role in the foraging, mating, and oviposition of H. armigera (Du, 2018;Nebapure, 2020). Cis-3-hexen-1-ol is a common odor of green leaves, and eugenol is a component of insect attractants (Ladd, 1984).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Efficacy of an improved method to screen semiochemicals of insect

Li¹,

Cao²,

Wang³

et al. 2021

PeerJ

View full text Add to dashboard Cite

Background A combination of gas chromatography-electroantennographic detection (GC-EAD) and gas chromatography-mass spectrometry (GC-MS) is typically used to screen active compounds that play a role in the regulation of insect behavior. This method uses two kinds of gas chromatography (GC) equipment and needs to compare compounds between the two chromatograms, and it is tedious and costly. To improve detection efficiency, as well as reduce costs and the rate of missed detection, we designed a system connecting gas chromatography (GC), electroantennography (EAG), and mass spectrometry (MS), with MS used instead of the flame ionization detector (FID) as the GC-EAD detector. To verify the feasibility of the improved method, we compare two methods—GC-EAG-MS and GC-EAD—through a series of experiments. Some researchers made similar improvements, but these were not compared with GC-EAD, and their method needed to be improved in the synchronization and split ratio. Our method has been optimized and improved in these aspects. Methods Helicoverpa armigera was the test organism; the improved method and conventional method were used to detect known and unknown compounds, as well as screen out active compounds that could generate responses in H. armigera antennae. Results Screening known single compounds using the two methods, the active compound benzaldehyde was detected in all seven concentrations of solution. By using the two methods, the five same active compounds of Helicoverpa armigera were detected in high concentration solution of the mixed compounds (100 mg L−1, 50 mg L−1); the four same active compounds were detected at 20 mg L−1 concentration; two identical same compounds were detected in low concentration solution (concentrations of 10 mg L−1 and below). By using the two methods, six identical active compounds of Helicoverpa armigera were detected in unknown compounds. Conclusion The improved method was consistent with the conventional method in terms of accuracy and sensitivity. However, compared with the traditional methods, Gas chromatography-electroantennographic-mass spectrometry (GC-EAG-MS) saved the cost of GC and FID equipment, thereby greatly lowering the experimental cost. In the experiment, GC-EAG-MS combined the two experimental operations of screening active substances by GC-EAD and identifying active substances by GC-MS into one, which not only reduced the experimental steps, but also avoided the false positive caused by the comparison of the two chromatograms, and it greatly reduced the difficulty level of the overall experimental analysis. GC-EAG-MS is more convenient, efficient, economical, and practical, and could confidently replace traditional methods. With further optimization, it could be widely applied in the study of plant and insect chemical ecology.

show abstract

Odor Perception in the Cotton Bollworm, Helicoverpa armigera, Exposed to Juglans regia, a Marginal Host Plant

et al. 2022

View full text Add to dashboard Cite

Electrophysiological Response of Two Moth Species, Helicoverpa armigera and Maruca vitrata to Host Plant (Cajanus cajan) Volatiles

Cited by 4 publications

References 10 publications

Effects of pear fruit‐derived volatiles and their concentrations on the responses of oriental fruit moths

Effects of pear fruit‐derived volatiles and their concentrations on the responses of oriental fruit moths

Efficacy of an improved method to screen semiochemicals of insect

Odor Perception in the Cotton Bollworm, Helicoverpa armigera, Exposed to Juglans regia, a Marginal Host Plant

Contact Info

Product

Resources

About