Electroporation in dense cell suspension—Theoretical and experimental analysis of ion diffusion and cell permeabilization

Pavlin, Mojca; Leben, Vilko; Miklavčič, Damijan

doi:10.1016/j.bbagen.2006.06.014

Cited by 91 publications

(74 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a sufficiently dense cell suspension, electroporation can also be monitored by measuring the bulk electric conductivity of the suspension, which increases significantly if a large fraction of the suspended cells is electroporated [55], [56]. In tissues, electroporation also affects the frequency dependence of their bulk electric conductivity and dielectric permittivity, and can thus be assessed by measuring the impedance spectrum of the tissue before and after exposure to electric pulses (and possibly also between consecutive pulses).…”

Section: Monitoring Of Electroporationmentioning

confidence: 99%

Cell membrane electroporation- Part 1: The phenomenon

Kotnik

Kramar

Pucihar

et al. 2012

IEEE Electr. Insul. Mag.

412

212

View full text Add to dashboard Cite

Section: Monitoring Of Electroporationmentioning

confidence: 99%

Cell membrane electroporation- Part 1: The phenomenon

Kotnik

Kramar

Pucihar

et al. 2012

IEEE Electr. Insul. Mag.

412

212

View full text Add to dashboard Cite

“…This phenomenon can be related to the formation of transient pores in the cell membranes by a process called electroporation. This enabled the transport of molecules and ions, known as the electro-osmosis effect, which increased the diffusion of materials (Lakkakula et al 2004;Pavlin et al 2007;Knirsch et al 2010). Additionally, in ohmic-heated hydro-distillation, the heat is generated volumetrically within the material.…”

Section: Scanning Electron Microscopy (Sem) Analysismentioning

confidence: 99%

Comparison of Citronella Oil Extraction Methods from Cymbopogon nardus Grass by Ohmic-heated Hydro-distillation, Hydro-Distillation, and Steam Distillation

Hamzah¹,

Man²,

Abidin³

et al. 2013

BioResources

View full text Add to dashboard Cite

A proposed method for citronella oil extraction was developed with the application of ohmic heated hydro-distillation. The objective was to compare the performance of three different extraction methods, viz. ohmic heated hydro-distillation, hydro-distillation, and steam distillation. The maximum amount of extracted oil yield by ohmic heated hydrodistillation was 7.64 mL/kWh as compared to hydro-distillation and steam distillation methods that resulted oil yields of 3.87 mL/kWh and 1.69 mL/kWh, respectively. The kinetics of extraction followed a second-order model. Gas chromatography-mass spectrometry analysis found that the major constituents of citronella oil (GC-MS) for the different extraction methods were citronellal, citronellol, and geraniol. Scanning electron microscopy (SEM) of citronella grass provided evidence that the lignocellulosic sources of the extracted citronella oil were schizogenous cavities and cellular lignin. The citronella that had undergone ohmicheated hydro-distillation and steam distillation showed some microfractures and less cell wall degradation than hydro-distillation. The cell walls were less rigid using ohmic-heated hydro-distillation compared to steam distillation. However, the cell walls of the hydro-distillation sample were less dense and exhibited pronounced swelling, but did not show any microfractures.

show abstract

“…For electroporation of cell suspensions, the proportion of the cells in the total volume is important. When they represent less than 1% of the volume fraction they behave as single cell, while for volume fraction greater than 10% or for clusters of cells, the induced transmembrane potential is affected by the suspension density (Susil et al 1998, Pavlin et al 2002Pavlin et al 2007). The fraction of electroporated cells decreases with increase in cell density and the resealing of cells in dense cell suspensions is slower.…”

Section: From Single-cell To Tissuementioning

confidence: 99%

“…The first phase of electroporation can be measured by changes in membrane conductivity and is related to short-lived transient pore formation, which does not contribute to molecular transport (Pavlin et al 2007). Molecular transport across the permeabilized cell membrane associated with electroporation is observed from the pore formation phase until membrane resealing is completed Teissié 1997, 1999;Prausnitz et al 1995;Puc et al 2003;Pavlin et al 2007).…”

Section: Basics and Mechanismsmentioning

confidence: 99%

Electroporation in Biological Cell and Tissue: An Overview

Kandušer

Miklavčič²

2008

Electrotechnologies for Extraction From Food Plants and Biomaterials

Self Cite

View full text Add to dashboard Cite

In this chapter, basics and mechanisms of electroporation are presented. Most important electric pulse parameters for electroporation efficiency for different applications that involve introduction of small molecules and macromolecules into the cell or cell membrane electrofusion are described. In all these applications, cell viability has to be preserved. However, in some biotechnological applications, such as liquid food sterilization or water treatment, electroporation is used as a method for efficient cell killing. For all the applications mentioned above, besides electric pulse parameters, other factors, such as electroporation medium composition and osmotic pressure, play significant roles in electroporation effectiveness. For controlled use of the method in all applications, the basic mechanisms of electroporation need to be known. The phenomenon was studied from the single-cell level and dense cell suspension that represents a simplified homogenous tissue model, to complex biological tissues. In the latter, different cell types and electric conductivity that change during the course of electric pulse application can significantly affect the effectiveness of the treatment. For such a complex situation, the design and use of suitable electrodes and theoretical modeling of electric field distribution within the tissue are essential. Electroporation as a universal method applicable to different cell types is used for different purposes. In medicine it is used for electrochemotherapy and genetherapy. In biotechnology it is used for water and liquid food sterilization and for transfection of bacteria, yeast, plant protoplast, and intact plant tissue. Understanding the phenomenon of electroporation, its mechanisms and optimization of all the parameters that affect electroporation is a prerequisite for successful treatment. In addition to the parameters mentioned above, different biological characteristics of treated cell affect the outcome of the treatment. Electroporation, gene electrotransfer and electrofusion are affected by cell membrane fluidity, cytoskeleton, and the presence of the cell wall in bacteria yeast and plant cells. Thus, electroporation parameters need to be specifically optimized for different cell types.

show abstract

Electroporation in dense cell suspension—Theoretical and experimental analysis of ion diffusion and cell permeabilization

Cited by 91 publications

References 44 publications

Cell membrane electroporation- Part 1: The phenomenon

Cell membrane electroporation- Part 1: The phenomenon

Comparison of Citronella Oil Extraction Methods from Cymbopogon nardus Grass by Ohmic-heated Hydro-distillation, Hydro-Distillation, and Steam Distillation

Electroporation in Biological Cell and Tissue: An Overview

Contact Info

Product

Resources

About