2012
DOI: 10.1038/gt.2012.15
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Electroporation-mediated gene transfer directly to the swine heart

Abstract: In vivo gene transfer to the ischemic heart via electroporation holds promise as a potential therapeutic approach for the treatment of heart disease. In the current study, we investigated the use of in vivo electroporation for gene transfer using 3 different penetrating electrodes and one non-penetrating electrode. The hearts of adult male swine were exposed through a sternotomy. Eight electric pulses synchronized to the rising phase of the R wave of the ECG were administered at varying pulse widths and field … Show more

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Cited by 45 publications
(32 citation statements)
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“…In contrast to previously reported needle-based electroporation devices232425, the ep-Patch was found to have the following prominent features; (i) Flexible parylene substrate (figure 1(a)) has the capability to match different surface profile of targeting tissue. (ii) It can electroporate in a non-invasive way.…”
Section: Resultscontrasting
confidence: 74%
“…In contrast to previously reported needle-based electroporation devices232425, the ep-Patch was found to have the following prominent features; (i) Flexible parylene substrate (figure 1(a)) has the capability to match different surface profile of targeting tissue. (ii) It can electroporate in a non-invasive way.…”
Section: Resultscontrasting
confidence: 74%
“…The drawbacks of the hybrid injection/electroporation approach limiting potential clinical use include possible ventricular fibrillation if the shock is not synchronized to ventricular electrical activation, the need to inject multiple times within the thin walled atria, and challenges of epicardial access (a limitation shared by the painting method). 41 …”
Section: Myocardial Gene Deliverymentioning
confidence: 99%
“…The artery was ligated to induce ischemia to the distal regions of the anterior left ventricle. Ventricular perfusion was assessed as described previously to determine the area of ischemia and loss of perfusion due LAD occlusion [9,10].…”
Section: Gene Electro Transfer To Porcine Myocardiummentioning
confidence: 99%
“…We have previously reported electro transfer mediated DNA delivery to the porcine heart [9,10]. Here we explore GET to the rat heart and a translational approach of GET to the porcine heart.…”
Section: Introductionmentioning
confidence: 99%