Electroporation of cell membranes supporting penetration of photodynamic active macromolecular chromophore dextrans

Lambreva, Maya D.; Glück, Brigitte; Radeva, Maria; Berg, Hermann

doi:10.1016/j.bioelechem.2003.10.007

Cited by 18 publications

(7 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Possibly, the increase of duration of electric pulses prolongs the lifetime of pores, and larger amount of the photosensitizer ALA can get into the cell at the same time. Lambreva et al (2004) used electroporation to deliver thyopyronin, trypan blue, and Cibacron-dextran to U-937 lymphoma and K-562 myeloid leukemia cells and found that access of even large molecules as Cibacrondextran can be augmented by electroporation (19). One of the main disadvantages of PDT is the slow accumulation of photosensitizers in the tumor tissues, and concentration needed for effective antitumor effects is often insufficient.…”

Section: Discussionmentioning

confidence: 99%

“…In that way, electroporation could help: a) to reduce accumulation duration of a photosensitizer, b) to minimize the effective dose of the photodrug, c) to reduce the phototoxic effect of photosensitized damage on the organism. Few recent studies performed in vitro and in vivo have shown that electroporation could increase the effectiveness of PDT (17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

et al. 2009

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Electroporation (Labanauskiene et al 2007;Lambreva et al 2004;Nolkrantz et al 2002;Underhill et al 2003;Xu et al 2007b) is an important bioengineering technique that has been used to deliver genes or anticancer drugs into the cytoplasm through the micropores on the cell membrane produced through electric stimulation and has been successfully utilized in photodynamic therapy. Xu et al reported for the first time that the combination of the electroporation and the conjugation of the TiO 2 nanoparticles with the monoclonal antibody could improve the photokilling selectivity and efficiency of photoexcited TiO 2 on cancer cells in PDT because the conjugation of the TiO 2 nanoparticles with monoclonal antibodies could increase the photokilling selectivity of TiO 2 nanoparticles to cancer cells and the electroporation could accelerate the delivery speed of the TiO 2 nanoparticles to cancer cells.…”

Section: Tio 2 Nanoparticlesmentioning

confidence: 99%

Nanoparticles for Photodynamic Therapy Applications

Vanderesse

Frochot

Barberi‐Heyob

et al. 2011

Intracellular Delivery

View full text Add to dashboard Cite

Photodynamic therapy has emerged as an alternative to chemo-and radiotherapy for the treatment of certain types of cancer. Nanoparticles have been used to improve the delivery and the efficiency of the photosensitizer as they allow its encapsulation without loss of activity. Using targeting strategies, they can also allow the selective accumulation of the photosensitizer in the cancer tissues. In this review, based on the chemical nature of the nanoparticles, the different methods for their syntheses are described from the pioneering works to the latest achievements. Indeed the nanosystems can be conjugated to a biomolecule or an antibody to target receptors over-expressed in cancer cells and/or angiogenic vascular endothelial cells. Numerous in vivo and in vitro applications have been described. Multifunctional nanoplatforms combining several applications within the same nano-object emerge as potential important theranostic tools.

show abstract

“…ECT has been used widely in the therapy of melanoma [1], but until now, EPDT has not been studied for this cancer. EPDT has been tested previously in vitro on several human tumors: lung cancer cells [1], histolytic lymphoma cells, chronic myeloid leukemia cells [33], and breast adenocarcinoma cell line [8]. Enhancement of Photofrin delivery by EP may increase the amount of photosensitizer incorporated, and the time and the site of its accumulation [8].…”

Section: Introductionmentioning

confidence: 98%

Effects of electrophotodynamic therapy in vitro on human melanoma cells – melanotic (MeWo) and amelanotic (C32)

Choromańska¹,

Kulbacka²,

Rembiałkowska³

et al. 2015

Melanoma Research

View full text Add to dashboard Cite

Photodynamic therapy has been considered ineffective for melanomas because of the competition between the absorbance of melanin from the melanoma and the absorbance of photosensitizers at the photosensitizer excitation light wavelength. Melanomas show considerable heterogeneity and resistance to phototherapy. The effectiveness of photodynamic therapy could be intensified by electroporation for enhanced transport of a photosensitizer by transient pores in the membrane. In this study, photodynamic therapy combined with electroporation was tested in vitro on the human melanoma cell lines melanotic melanoma (MeWo) and amelanotic melanoma (C32). Control experiments were conducted on human keratinocytes (HaCaT). Photofrin was used as a photosensitizer. Photosensitizer distribution, cloning efficacy test, comet assay, and assessment of apoptotic proteins were performed. Melanin levels were determined before and after photodynamic therapy. The experiments indicated that electroporation effectively supports the photodynamic method. It was found that photodynamic therapy with electroporation efficiently induces apoptosis in melanotic and amelanotic melanoma cells.

show abstract

Electroporation of cell membranes supporting penetration of photodynamic active macromolecular chromophore dextrans

Cited by 18 publications

References 8 publications

Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

Enhancement of photodynamic tumor therapy effectiveness by electroporation in vitro

Nanoparticles for Photodynamic Therapy Applications

Effects of electrophotodynamic therapy in vitro on human melanoma cells – melanotic (MeWo) and amelanotic (C32)

Contact Info

Product

Resources

About