Electroretinographic Response in WAG/Rij Rats after Low-Intensity Cyclic Light Exposure

Cillino, Salvatore; Guarneri, Rosa; Guarneri, P.; Pennica, C; Chichi, G; Piccoli, Federico; Ponte, F.

doi:10.1159/000267282

Cited by 5 publications

(3 citation statements)

References 4 publications

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“…A reduction in ERG amplitudes with age has also been shown previously for both pigmented and nonpigmented mice and rats (e.g. Cillino et al, 1993;Li et al, 2001).…”

Section: Rod Erg Analysis In Pigmented and Albino Animals During Matusupporting

confidence: 78%

“…These possibilities, however, cannot be distinguished with the present data. Our results differ from data obtained from guinea pigs in which albino guinea pigs had faster responses than pigmented animals (Vingrys & Bui, 2001), but correlates with data obtained by multifocal ERGs from albino and pigmented rats showing that response latency is increased and amplitude reduced in albino rats (Ball & Petry, 2000), and obtained by single-flash ERGs from albino rats demonstrating that b-wave latency increased with age (Cillino et al, 1993). These differences between various rodents have yet to be addressed.…”

Section: Rod Erg Analysis In Pigmented and Albino Animals During Matusupporting

confidence: 49%

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Structure–function analysis of rods and cones in juvenile, adult, and aged C57BL/6 and Balb/c mice

et al. 2003

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To determine whether the photoreceptors change structurally and functionally during aging, and to analyze whether pigmentation in the retinal pigment epithelium might be a contributing factor. Young, adult, and aged C57BL/6 and Balb/c mice (1, 4, and 17 months of age) were housed under a 12-h light/12-h dark cycle, with an ambient light intensity at the eye level of the mice of 85 +/- 18 lux. Scotopic single-flash and photopic-flicker electroretinograms (ERGs) after complete dark adaptation were used to assess rod and cone function, respectively. Numbers of rod photoreceptors were counted in plastic sections, and rhodopsin levels were measured using absorption difference spectrophotometry. Numbers and types of cones were determined using lectin staining in retinal flatmounts and cone-specific antibodies in radial frozen sections. Young pigmented C57BL/6 and nonpigmented Balb/c mice had similar numbers of rods. In both mouse strains, there was an overall decline in rod photoreceptor number during aging, which was more pronounced in albino mice. Rod cell numbers correlated with a drop in the overall amount of rhodopsin and a reduction in the maximum a-wave of the rod ERG. The number of short-wavelength cones was unaffected by age and pigmentation, whereas an age-related decline was observed in mid-wavelength (MWL) cones in albino, but not in pigmented mice. In contrast, MWL cone function was reduced during aging in both strains. Flicker-fusion frequency was determined to be approximately 10 Hz lower in albino animals, which is due to prolonged b-waves in these ERGs. Age-related changes were found in both photoreceptor systems, rods and cones, and in both pigmented and nonpigmented mice. However, rod photoreceptors appear to be more susceptible to both aging and the lack of pigmentation, when compared to cones. These results may help as we begin to understand certain age-related retinal diseases.

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“…A reduction in ERG amplitudes with age has also been shown previously for both pigmented and nonpigmented mice and rats (e.g. Cillino et al, 1993;Li et al, 2001).…”

Section: Rod Erg Analysis In Pigmented and Albino Animals During Matusupporting

confidence: 78%

Section: Rod Erg Analysis In Pigmented and Albino Animals During Matusupporting

confidence: 49%

Structure–function analysis of rods and cones in juvenile, adult, and aged C57BL/6 and Balb/c mice

et al. 2003

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“…et al (1980) [3,4] reported, that retinal degeneration in Waj/Rij rats resembles RP temporally and morphologically. In WAG/Rij rat, reared under homogenous lowintensity cyclic light exposure, the b-wave amplitude of electroretinogram decreased, and its implicit time increased in the older age groups significantly more than in Wistar rats [5] . Prolonged implicit time and decreased b-wave amplitude of electroretinogram were shown in most RP cases compared with that in normal eyes [6] .…”

Section: Introductionmentioning

confidence: 84%

Glial fibrillary acidic protein expression in postnatal development of WAG/Rij rat retina

Musina¹,

Baygildin

Хисматуллина

2020

Egyptian Journal of Histology

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Introduction: WAG/Rij rat is an experimental model of retinal degenerative diseases intensifying with age. Degeneration begins in the photoreceptor cells layer, followed by disappearance of photoreceptor cells, migration of the pigment epithelium and disorganization of remaining retinal layers. However, the age of onset of the degeneration is unknown. The destroyed neurons of retina are usually replaced by glial cells. A typical marker for several types of glial cells is the glial fibrillary acidic protein (GFAP). Aim of the Work:The aim of the study is to determine postnatal changes of GFAP expression in retina of WAG/Rij rats. Material and Methods: WAG/Rij rats of both sexes and different ages: 1, 10, 15, 20, 30, 60, 180, 360 postnatal days (P) (n =3 for each age) were taken. The immunohistochemical analysis of GFAP expression in the retina of WAG/Rij rats was performed and the expression area was measured. Results: Expression of GFAP in the retina is detected from P1 to P20 as a brown staining in the internal limiting membrane or as a brown lines on the inner retina. On P30 the glial fibrillary acidic protein is already detected in significant quantities in almost all layers of the retina of the experimental rats. From P60 to P360 the expression of GFAP in the retina of the WAG/ Rij rats is more pronounced and increases in direct proportion with the age. Conclusion: It was found that GFAP expression in the retina of WAG/Rij rats increases in direct proportion to the age. The increasing GFAP expression in the retina of the WAG/Rij rats starting from the 30th postnatal day indicates an intensification of destructive processes in retinal neurons with age and their replacement by Müller cells.

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The ERG, EOG, and VEP in Rats

1995

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Electroretinographic Response in WAG/Rij Rats after Low-Intensity Cyclic Light Exposure

Cited by 5 publications

References 4 publications

Structure–function analysis of rods and cones in juvenile, adult, and aged C57BL/6 and Balb/c mice

Structure–function analysis of rods and cones in juvenile, adult, and aged C57BL/6 and Balb/c mice

Glial fibrillary acidic protein expression in postnatal development of WAG/Rij rat retina

The ERG, EOG, and VEP in Rats

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