Electrospinning and dual crosslinking of water-soluble silk fibroin modified with glycidyl methacrylate

Bae, Su Bin; Kim, Min Hee; Park, Won Ho

doi:10.1016/j.polymdegradstab.2020.109304

Cited by 34 publications

(30 citation statements)

References 33 publications

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“…Bae et al (2020) [ 125 ] prepared a water-soluble SF derivative by the methacrylation (introduction of glycidyl methacrylate—GMA) of SF’s reactive side chains (−NH 2 , −OH, −COOH). Through a green, eco-friendly electrospinning process using distilled water as a spinning solvent, GMA-modified SF water-soluble ultrathin fibers were obtained.…”

Section: Sf As a Green Materialsmentioning

confidence: 99%

“…Next, a structural change from random coils to b-sheets was facilitated by the treatment with an aqueous ethanol solution in order to insolubilize the fibers, and chemical crosslinking improved the water resistance. Electrospinning is a suitable alternative for the green processing of SF without the use of toxic substances [ 125 ]. Another study by Yang et al (2017) [ 10 ] reported the feasibility of developing natural green composite matrices based on SF and MN (Manuka honey), used as an antimicrobial dressing in wound healing (ulcer).…”

Section: Sf As a Green Materialsmentioning

confidence: 99%

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The Contribution of Silk Fibroin in Biomedical Engineering

Lujerdean

Baci

Cucu

et al. 2022

Insects

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Silk fibroin (SF) is a natural protein (biopolymer) extracted from the cocoons of Bombyx mori L. (silkworm). It has many properties of interest in the field of biotechnology, the most important being biodegradability, biocompatibility and robust mechanical strength with high tensile strength. SF is usually dissolved in water-based solvents and can be easily reconstructed into a variety of material formats, including films, mats, hydrogels, and sponges, by various fabrication techniques (spin coating, electrospinning, freeze-drying, and physical or chemical crosslinking). Furthermore, SF is a feasible material used in many biomedical applications, including tissue engineering (3D scaffolds, wounds dressing), cancer therapy (mimicking the tumor microenvironment), controlled drug delivery (SF-based complexes), and bone, eye and skin regeneration. In this review, we describe the structure, composition, general properties, and structure–properties relationship of SF. In addition, the main methods used for ecological extraction and processing of SF that make it a green material are discussed. Lastly, technological advances in the use of SF-based materials are addressed, especially in healthcare applications such as tissue engineering and cancer therapeutics.

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Section: Sf As a Green Materialsmentioning

confidence: 99%

Section: Sf As a Green Materialsmentioning

confidence: 99%

The Contribution of Silk Fibroin in Biomedical Engineering

Lujerdean

Baci

Cucu

et al. 2022

Insects

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“…Gelatin is commonly modified with methacrylate moieties to create a material (GelMA) that can be stabilized by photoinitiated crosslinking of electrospun fibers. [115][116][117] Similar chemistry has been used to modify HA, 30,118 silk fibroin, 119,120 and PEG. 32,94 Dextran, another polysaccharide, can also be modified with methacrylate 2,3,31 or vinyl sulfone 87 functional groups for crosslinking and subsequent reactions that aim at improving bioactivity.…”

Section: Chemical Modifications For Covalent Crosslinking Of Hydrophilic Polymersmentioning

confidence: 99%

Electrospun hydrogels for dynamic culture systems: advantages, progress, and opportunities

Grewal

Highley

2021

Biomater. Sci.

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“…Because of the established history of B. mori species in the textile trade its cocoon production is in large supply, it is also renewable, recyclable, and biodegradable. 2 , 3 As such, it has been utilized in a number of tissue scaffolding applications such as in the cornea, skin, bone, cardiac patches, and periodontics, as well as drug delivery and, lately, more broadly in medicine, in the field of bioelectronics. 4 − 11 …”

Section: Introductionmentioning

confidence: 99%

Facile One-Pot Method for All Aqueous Green Formation of Biocompatible Silk Fibroin-Poly(Ethylene Oxide) Fibers for Use in Tissue Engineering

Heseltine

Bayram

Gultekinoglu

et al. 2022

ACS Biomater. Sci. Eng.

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Silk fibroin (SF) fibers are highly regarded in tissue engineering because of their outstanding biocompatibility and tunable properties. A challenge remains in overcoming the trade-off between functioning and biocompatible fibers and the use of cytotoxic, environmentally harmful organic solvents in their processing and formation. The aim of this research was to produce biocompatible SF fibers without the use of cytotoxic solvents, via pressurized gyration (PG). Aqueous SF was blended with poly(ethylene oxide) (PEO) in ratios of 80:20 (labeled SF-PEO 80:20) and 90:10 (labeled SF-PEO 90:10) and spun into fibers using PG, assisted by a range of applied pressures and heat. Pure PEO (labeled PEO-Aq) and SF solubilized in hexafluoro-isopropanol (HFIP) (labeled SF-HFIP) and aqueous SF (labeled SF-Aq) were also prepared for comparison. The resulting fibers were characterized using SEM, TGA, and FTIR. Their in vitro cell behavior was analyzed using a Live/Dead assay and cell proliferation studies with the SaOS-2 human bone osteosarcoma cell line (ATCC, HTB-85) and human fetal osteoblast cells (hFob) (ATCC, CRL-11372) in 2D culture conditions. Fibers in the micrometer range were successfully produced using SF-PEO blends, SF-HFIP, and PEO-Aq. The fiber thickness ranged from 0.71 ± 0.17 μm for fibers produced using SF-PEO 90:10 with no applied pressure to 2.10 ± 0.78 μm for fibers produced using SF-PEO 80:10 with 0.3 MPa applied pressure. FTIR confirmed the presence of SF via amide I and amide II bands in the blend fibers because of a change in structural conformation. No difference was observed in thermogravimetric properties among varying pressures and no significant difference in fiber diameters for pressures. SaOS-2 cells and hFOb cell studies demonstrated higher cell densities and greater live cells on SF-PEO blends when compared to SF-HFIP. This research demonstrates a scalable and green method of producing SF-based constructs for use in bone-tissue engineering applications.

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Electrospinning and dual crosslinking of water-soluble silk fibroin modified with glycidyl methacrylate

Cited by 34 publications

References 33 publications

The Contribution of Silk Fibroin in Biomedical Engineering

The Contribution of Silk Fibroin in Biomedical Engineering

Electrospun hydrogels for dynamic culture systems: advantages, progress, and opportunities

Facile One-Pot Method for All Aqueous Green Formation of Biocompatible Silk Fibroin-Poly(Ethylene Oxide) Fibers for Use in Tissue Engineering

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