Electrospinning of poly(hydroxybutyrate‐<i>co</i>‐hydroxyvalerate) fibrous tissue engineering scaffolds in two different electric fields

Tong, Ho‐Wang; Wang, Min

doi:10.1002/pen.21937

Cited by 14 publications

(10 citation statements)

References 35 publications

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“…Other studies using other solvent systems report that PHBV can be electrospun as fibres without beads or polymer pearls. 10,11 If thicker scaffolds are required vapour and heat annealing can be employed to anneal layers of scaffolds together (see Figure 4). These scaffold layers do not delaminate and it can be very difficult to find the junction between layers.…”

Section: Representative Resultsmentioning

confidence: 99%

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Bye

Wang²,

Bullock

et al. 2012

JoVE

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Electrospinning is a commonly used and versatile method to produce scaffolds (often biodegradable) for 3D tissue engineering.(1, 2, 3) Many tissues in vivo undergo biaxial distension to varying extents such as skin, bladder, pelvic floor and even the hard palate as children grow. In producing scaffolds for these purposes there is a need to develop scaffolds of appropriate biomechanical properties (whether achieved without or with cells) and which are sterile for clinical use. The focus of this paper is not how to establish basic electrospinning parameters (as there is extensive literature on electrospinning) but on how to modify spun scaffolds post production to make them fit for tissue engineering purposes--here thickness, mechanical properties and sterilisation (required for clinical use) are considered and we also describe how cells can be cultured on scaffolds and subjected to biaxial strain to condition them for specific applications. Electrospinning tends to produce thin sheets; as the electrospinning collector becomes coated with insulating fibres it becomes a poor conductor such that fibres no longer deposit on it. Hence we describe approaches to produce thicker structures by heat or vapour annealing increasing the strength of scaffolds but not necessarily the elasticity. Sequential spinning of scaffolds of different polymers to achieve complex scaffolds is also described. Sterilisation methodologies can adversely affect strength and elasticity of scaffolds. We compare three methods for their effects on the biomechanical properties on electrospun scaffolds of poly lactic-co-glycolic acid (PLGA). Imaging of cells on scaffolds and assessment of production of extracellular matrix (ECM) proteins by cells on scaffolds is described. Culturing cells on scaffolds in vitro can improve scaffold strength and elasticity but the tissue engineering literature shows that cells often fail to produce appropriate ECM when cultured under static conditions. There are few commercial systems available that allow one to culture cells on scaffolds under dynamic conditioning regimes--one example is the Bose Electroforce 3100 which can be used to exert a conditioning programme on cells in scaffolds held using mechanical grips within a media filled chamber.(4) An approach to a budget cell culture bioreactor for controlled distortion in 2 dimensions is described. We show that cells can be induced to produce elastin under these conditions. Finally assessment of the biomechanical properties of processed scaffolds cultured with or without cells is described.

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Section: Representative Resultsmentioning

confidence: 99%

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Bye

Wang²,

Bullock

et al. 2012

JoVE

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“…Electrospinning microporous membranes with high waterproofness and good breathability have attracted tremendous attention, owning to the characteristics that can restrict the permeation of liquids and toxic gas, but allow the water vapor to escape . In general, they have a lot of potential applications such as protective clothing, membrane distillation, filter, and tissue engineering . According to the previous studies, methods for fabrication of the membranes with excellent hydrophobicity were mainly done by decorating the surface with low‐surface energy materials and controlling the surface structure of the membranes.…”

Section: Introductionmentioning

confidence: 99%

Preparation of electrospun polyurethane/hydrophobic silica gel nanofibrous membranes for waterproof and breathable application

Cao

et al. 2017

Polymer Engineering & Sci

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The polyurethane (PU)/hydrophobic silica gel (HSG) fibrous membranes with the hydrophobic and breathable surface was fabricated via electrospinning. By employing the HSG incorporation, mechanical properties, thermal stability, and waterproofness of the composite membranes could be improved. The porous structure of the membranes would be regulated by tuning the temperatures of thermal treatment. When HSG content increased to 3 wt%, the PU/HSG composite membranes possessed remarkable tensile strength of 6.3 MPa and high water contact angle (WCA) of 134°. Furthermore, the maximum WCA (142° ± 1°), good hydrostatic pressure (5.45 kPa), large water vapor transmission rate (8.05 kg/m2/day), and high air permeability (9.25 L/m2/s) of the composite membranes could be achieved by heat treatment at 120°C. The resultant membranes performed significantly better when compared to the pure PU membranes under the same conditions, such as the higher tensile strength (100%), better waterproofness (200%), and stronger breathablity (25%). POLYM. ENG. SCI., 58:1381–1390, 2018. © 2017 Society of Plastics Engineers

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“…As fiber diameter is reduced from micrometer to nanometer scale, many unique properties appear such as high specific surface area and flexibility in surface functionalities . These properties of nanofibers make them useful for many applications such as filtration , tissue scaffolds , sensors , drug delivery , and wound dressing . Different techniques are reported for preparing polymer nanofibers, amongst which electrospinning process has been accepted as a simple and efficient method.…”

Section: Introductionmentioning

confidence: 99%

The effect of MWNTs concentration and nanofiber orientation on mechanical properties of PAA nanocomposite nanofibrous web

et al. 2015

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In this research, nanocomposite nanofibrous webs of poly(acrylic acid) (PAA)/multi‐walled carbon nanotubes (MWNTs) were obtained via electrospinning. The effect of MWNTs concentration on the morphology and mechanical properties of PAA/MWNTs nanofibers was investigated by changing the MWNTs content from 0 to 5 wt%. The results showed that average diameter of nanofibers increased with increasing the MWNTs concentration and presence of MWNTs led to the enhancement of mechanical properties. Also, the results revealed that the strength, modulus, and elongation at break of samples increased 3.22, 2.70, and 4.27 fold, respectively, after adding 3 wt% of MWNTs. In addition, the effect of rotating speed of collector on the orientation of PAA nanofibers and its effect on mechanical properties was investigated. Scanning electron microscopy (SEM) studies demonstrated that the degree of nanofibers orientation increased with the augmentation of drum speed to 25 rps. Moreover, the average nanofibers diameter decreased with the increase of drum speed. Improvement of nanofiber orientation resulted in superior mechanical properties that is, higher strength and modulus of aligned nanofiber layers were obtained in comparison to nonaligned layers (12.6 and 26.6 fold, respectively). POLYM. COMPOS., 37:3149–3159, 2016. © 2015 Society of Plastics Engineers

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Electrospinning of poly(hydroxybutyrate‐co‐hydroxyvalerate) fibrous tissue engineering scaffolds in two different electric fields

Cited by 14 publications

References 35 publications

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Preparation of electrospun polyurethane/hydrophobic silica gel nanofibrous membranes for waterproof and breathable application

The effect of MWNTs concentration and nanofiber orientation on mechanical properties of PAA nanocomposite nanofibrous web

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