Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro

Min, Byung‐Moo; Lee, Gene; Kim, So Hyun; Nam, Young Sik; Lee, Taek Seung; Park, Won Ho

doi:10.1016/j.biomaterials.2003.08.045

Cited by 1,061 publications

(609 citation statements)

References 17 publications

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“…Moreover, cell growth on electrospun scaffolds was better than that on the film scaffolds (Figure 4, PLA vs. PLAF, SHPXL, XL-L, XL-M, and XL-H vs. ZFXL), since the porous structure of electrospun fiber mats was able to facilitate the transportation of oxygen, nutrients, and the metabolic waste of cells, and the migration of cells and communication between them, all of which contribute to better cell growth [15,[39][40][41]. Furthermore, after 24h fewer cells were growing on the electrospun zein fibers cross-linked using SHP (SHPXL) than on samples XL-L, XL-M, and XL-H cross-linked without SHP.…”

Section: Cell Growthmentioning

confidence: 99%

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

2010

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Section: Cell Growthmentioning

confidence: 99%

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

2010

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“…Fibers fabricated by an electrically driven jet have been employed in various biomedical applications such as tissue engineered constructs and wound healing patches. 16 One of the advantages of ESFs is that a wide variety of macromolecules can be used for fabrication from biologically derived polymers such as gelatin, collagen, silk fibroin, chitosan, and cellulose [17][18][19][20][21] to synthetic polymers such as polyglycolide, poly (L-lactide) (PLLA), poly(e-caprolactone) (PCL), polyurethane, and poly(vinyl alcohol). 22 ESFs made of natural polymers usually possess inferior mechanical properties, while those made of synthetic polymers often lack suitable biological activities.…”

mentioning

confidence: 99%

Electrospun PLGA Fibers Incorporated with Functionalized Biomolecules for Cardiac Tissue Engineering

Lee

Lin

et al. 2014

Tissue Engineering Part A

102

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Structural similarity of electrospun fibers (ESFs) to the native extracellular matrix provides great potential for the application of biofunctional ESFs in tissue engineering. This study aimed to synthesize biofunctionalized poly (Llactide-co-glycolide) (PLGA) ESFs for investigating the potential for cardiac tissue engineering application. We developed a simple but novel strategy to incorporate adhesive peptides in PLGA ESFs. Two adhesive peptides derived from laminin, YIGSR, and RGD, were covalently conjugated to poly-L-lysine, and then mingled with PLGA solution for electrospinning. Peptides were uniformly distributed on the surface and in the interior of ESFs. PLGA ESFs incorporated with YIGSR or RGD or adsorbed with laminin significantly enhanced the adhesion of cardiomyocytes isolated from neonatal rats. Furthermore, the cells were found to adhere better on ESFs compared with flat substrates after 7 days of culture. Immunofluorescent staining of F-actin, vinculin, a-actinin, and Ncadherin indicated that cardiomyocytes adhered and formed striated a-actinin better on the laminin-coated ESFs and the YIGSR-incorporated ESFs compared with the RGD-incorporated ESFs. The expression of a-myosin heavy chain and b-tubulin on the YIGSR-incorporated ESFs was significantly higher compared with the expression level on PLGA and RGD-incorporated samples. Furthermore, the contraction of cardiomyocytes was faster and lasted longer on the laminin-coated ESFs and YIGSR-incorporated ESFs. The results suggest that aligned YIGSRincorporated PLGA ESFs is a better candidate for the formation of cardiac patches. This study demonstrated the potential of using peptide-incorporated ESFs as designable-scaffold platform for tissue engineering.

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“…[103,104] Silk is degradable and lightweight with excellent thermal and mechanical properties. [105,106] SF is obtained after the extraction of sericin proteins from silk.…”

Section: Sfmentioning

confidence: 99%

Graphene Oxide/Polymer‐Based Biomaterials

2017

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Since its discovery in 2004, derivatives of graphene have been developed and heavily investigated in the field of tissue engineering. Among the most extensively studied forms of graphene, graphene oxide (GO), and GO/ polymer-based nanocomposites have attracted great attention in various forms such as films, 3D porous scaffolds, electrospun mats, hydrogels, and nacre-like structures. In this review, the most actively investigated GO/ polymer nanocomposites are presented and discussed, these nanocomposites are based on chitosan, cellulose, starch, alginate, gellan gum, poly(vinyl alcohol) (PVA), poly(acrylamide), poly(e-caprolactone) (PCL), poly(lactic acid) (PLLA), poly(lactide-co-glycolide) (PLGA), gelatin, collagen, and silk fibroin (SF). The biological and mechanical performance of such nanocomposites are comprehensively scrutinized and ongoing research questions are addressed. The analysis of the literature reveals overall the great potential of GO/ polymer nanocomposites in tissue engineering strategies and indicates also a series of challenges requiring further research efforts.

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Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro

Cited by 1,061 publications

References 17 publications

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

Cytocompatible cross-linking of electrospun zein fibers for the development of water-stable tissue engineering scaffolds

Electrospun PLGA Fibers Incorporated with Functionalized Biomolecules for Cardiac Tissue Engineering

Graphene Oxide/Polymer‐Based Biomaterials

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