Electrospray ionization mass spectrometry fingerprinting of extracts of the leaves of Arrabidaea chica

Abstract

Arrabidaea chica (crajiru) é uma importante planta da Amazônia. Seus extratos são usados como pigmento, agentes antimicrobianos e adstringentes. Três variedades diferentes desta espécie são cultivadas na região Amazônica. Neste trabalho, infusões diretas de extratos de A. chica destas três variedade foram analisadas por impressão digital via espectrometria de massas com ionização por eletrospray (ESI(+)-MS). Dados derivados destes espectros foram classificados utilizando um método de análise multivariada (PLS-DA, análise discriminante com calibração multivariada por mínimos quadrados parciais). O método direto aqui apresentado baseia-se na extração de folhas secas e em pó com uma solução de metanol/água acidificada sem manipulação adicional da amostra. A solução sobrenadante foi analisada através de infusão direta por ESI(+)-MS, o que resultou em perfis de composição para cada variedade. 3-Desoxiantocianidinas são importantes substâncias presentes na A. chica, seus íons foram usados como marcadores no tratamento de dados utilizando PLS-DA, o que possibilitou a separação das três variedades. Impressão digital por ESI(+)-MS funciona como um método simples e rápido para diferenciação de variedades de A. chica.Arrabidaea chica (crajiru) is an important Amazonian plant. Its extracts are used as red pigments, antimicrobial agents and astringents. Three different varieties of this species are cultivated in the Amazon region. In this work, direct infusions of A. chica extracts from these three varieties were analyzed via electrospray ionization mass spectrometry (ESI(+)-MS) fingerprinting. Derived data from the spectra were classified by using a multivariate method (PLS-DA, partial least squares-discriminant analysis). The direct method that is herein presented relies on extraction of dry, powdered leaves with acidified methanol/water solution with no further sample preparation. The resulting supernatants were analyzed by direct infusion ESI(+)-MS, which provides characteristic fingerprints of the sample composition. 3-Deoxyanthocyanidins are important substances in A. chica, their ions were used as markers in the PLS-DA data treatment. PLS-DA was able to differentiate the three varieties. ESI(+)-MS fingerprinting works as a simple and fast method to differentiate varieties of A. chica.

Antioxidant Capacity of the Leaf Extract Obtained from Arrabidaea chica Cultivated in Southern Brazil

“…Fractions 7 and 8 yielded scutellarein ( 1 ) (15 mg) and fraction 9 afforded apigenin ( 2 ) (10 mg). The compounds were identified by ESI-MS-MS, 1 H- and 13 C-NMR spectral data, and by comparison with data available in the literature [11], [12], [15]–[20].…”

Quantitation of organic acids in wine and grapes by direct infusion electrospray ionization mass spectrometry

“…The main advantages of ESI-MS are that neither chromatographic separation nor derivatization is needed, having minimal or no sample pretreatment at all, being also relatively simple and rapid. Our group and others have been successfully applying this method for the ngerprinting characterization of several complex mixtures such as those found in propolis, 11 plant extracts, 12 wine, 4,[13][14][15] coffee, 16 biodiesel, 17 fruit juices, 18,19 and essential oils. 20 We have already applied direct infusion ESI-MS ngerprinting for wine analysis, more specically to characterize samples of must from six grape varieties during the fermentation process and samples of their respective wines aer malolactic fermentation.…”

Chemical Characterization and Anti-inflammatory Assessment of the Hydroethanolic Extract of Fridericia chica

“…The presence of flavonoids has been reported in the Amazonian herbal drug pariri (Takemura et al 1995;Zorn et al 2001;Devia et al 2002;Schiozer et al 2012;Siraichi et al 2013), but little else is known about the micromolecular composition of its extracts and the association with its probable intestinal anti-inflammatory potential. Five solvents were used to optimize the extraction of phenolic compounds present in the leaves (boiling water, ethanol, and mixtures of ethanol:water in the proportions of 1:1, 7:3, and 8:2).…”