Electrospray Liquid Chromatography/Mass Spectrometry of Ginsenosides

Breemen, Richard B. van; Huang, Chao; Lu, Zhi Zhen; Rimando, Agnes M.; Fong, Harry H. S.; Fitzloff, John F.

doi:10.1021/ac00117a026

Cited by 65 publications

(47 citation statements)

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“…The method validation of the quantitative assay was described previously [13]. Negative ESI was used in this study, although positive ion ESI also provided good sensitivity for the detection of ginsenosides [14][15][16][18][19][20][21][22][23]. It was found that the application of negative ion mode provided significant advantages for analyzing the ginsenoside in the biological samples, especially for the feces samples.…”

Section: Lc-ms Analysis Of Rg 3 In Rat Biological Samplesmentioning

confidence: 94%

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In vivo rat metabolism and pharmacokinetic studies of ginsenoside Rg3

Qian

Cai

Wong

et al. 2005

Journal of Chromatography B

103

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Section: Lc-ms Analysis Of Rg 3 In Rat Biological Samplesmentioning

confidence: 94%

“…LC-MS has been proved to be a powerful and reliable analytical approach for supporting studies on active components in Chinese medicines [13][14][15][16][17][18][19]. Both positive and negative ESI-MS and MS-MS have been applied for analyzing various ginsenosides extracted from Oriental and North American ginsengs [20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

In vivo rat metabolism and pharmacokinetic studies of ginsenoside Rg3

Qian

Cai

Wong

et al. 2005

Journal of Chromatography B

103

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“…MS. 22 Applying the on-column polymerization reaction to the monolithic silica capillary columns modified with MAS as an universal anchor group, resulted in highly efficient weak anionexchange (WAX) and strong anion-exchange (SAX) columns. The monolithic WAX and SAX columns, 200H-DAHMA and 200H-DMAEA-Q, were examined to facilitate the separation of mixtures of inorganic anions, as shown in Figs.…”

Section: Anion-exchange Modementioning

confidence: 99%

Anion- and Cation-Exchange MicroHPLC Utilizing Poly(methacrylates)-coated Monolithic Silica Capillary Columns

et al. 2007

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The preparations of micro-sized monolithic silica columns have recently been attracting attention due to the potential of their multi-mode application for microHPLC, 1,2 LC-MS, 3,4 and 2D HPLC separations 5-7 with higher column efficiencies than the conventional particle-packed columns exhibit. Until very recently, the surface modifications of the monolithic silica columns were limited to the reversed-phase (RP) mode in many cases, except for an aminopropyl bonded column 8 or chiral separation columns. 9In 2006, a hydrophilic interaction chromatography (HILIC)-type monolithic silica column 10 and a weak cation-exchange (WCX) monolithic silica column 11 were reported. A highly efficient monolithic silica column modified with strong cation-exchange (SCX) functionality was also reported. 12 Under the RP mode separation, the monolithic silica columns achieved much higher separation efficiency with higher theoretical plates (can be larger than 100000 plates) 13 and much faster separation than the conventional particle-packed columns could achieve. However, the improvement of the column efficiency for ion-exchange chromatography has been limited to polymer monolithic columns possessing ion-exchange functionalities. In many cases, polymer monolithic columns are evaluated by the electrochromatography mode, 14 and it is not easy to adapt them to the common HPLC system. The ionexchange chromatography is widely used for the proteomics, 15,16 the separation of lipooligosaccharides 17 and NAHoligosaccharides, 18 quantification of proteins, 19 and metabolomics studies. 20 The preparation of highly efficient ionexchange columns will thus be beneficial to the progress of the above-mentioned life sciences areas.To realize higher separation efficiency in ion-exchange modes than conventional particle-packed columns, researchers have suggested that the functionalization of monolithic silica capillary columns by a polymer-coating reaction will be a reasonable method for WCX mode. 11 In this report, the ionexchange mode was enlarged to SCX and strong-and weak anion-exchange (SAX and WAX) modes, to show the utility of the present polymer-coating method for the column functionalization and for providing much more efficient anionexchange columns. ExperimentalReagents and chemicals 3-Methacryloxypropyltriethoxysilane (MAS) was prepared by the previously reported method. 10Reagent grade methanol (Nacalai Tesque, Kyoto, Japan) was used after single distillation. Reagent grade toluene (Nacalai) was distilled from calcium hydride. 3-Diethylamino-2-hydroxypropyl methacrylate (DAHMA) was prepared by reacting glycidyl methacrylate (2.7 ml, 20 mmol, Wako Pure Chemical Industries Ltd., Osaka, Japan) and diethylamine (4.1 ml, 40 mmol, Nacalai) under reflux for 24 h, followed by a removal of the excess of diethylamine under reduced pressure. 21Other monomers: 2-(triethylammonium)ethyl methacrylate chloride (DMAEA-Q, Kohjin, Tokyo, Japan), p-styrenesulfonic acid sodium salt (pSSA, Tokyo Chemical Industry Co. Ltd., Tokyo, Japan) and 2-acrylamido-2-me...

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“…The use of a mass spectrometer as a detector for HPLC has increasingly gained acceptance, complementing or replacing conventional detection methods, such as UV absorbance, electrochemical detection or laser-induced fluorescence, which have proven to be less informative and less universal. [11][12][13] Among the various mass spectrometric approaches, electrospray ionization mass spectrometry (ESI-MS) is considered to be the best to couple with HPLC. It has been successfully used for the determination of ginsenosides 13 and cardiac glycosides.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Paeoniae Radix by High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

Zhao

Sun

2003

ANAL. SCI.

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A method has been developed for the qualitative analysis of paeonol, paeoniflorin and their derivatives in Paeoniae Radix by high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). Gradient elution with acetonitrile-water solvent system was employed in an HPLC-ESI-MS study. The negative-ion ESI mode was suitable for these compounds. The peaks were identified by their mass spectra, UV spectra and fragments of their MS 2 spectra. The structures of three unknown compounds are inferred in this paper.

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Electrospray Liquid Chromatography/Mass Spectrometry of Ginsenosides

Cited by 65 publications

References 0 publications

In vivo rat metabolism and pharmacokinetic studies of ginsenoside Rg3

In vivo rat metabolism and pharmacokinetic studies of ginsenoside Rg3

Anion- and Cation-Exchange MicroHPLC Utilizing Poly(methacrylates)-coated Monolithic Silica Capillary Columns

Analysis of Paeoniae Radix by High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry

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