Electrospun chitosan/polyvinyl alcohol nanofibre mats for wound healing

Abstract

Chitosan (CS) aqueous salt blended with polyvinyl alcohol (PVA) nanofibre mats was prepared by electrospinning. CS was dissolved with hydroxybenzotriazole (HOBt), thiamine pyrophosphate (TPP) and ethylenediaminetetraacetic acid (EDTA) in distilled water without the use of toxic or hazardous solvents. The CS aqueous salts were blended with PVA at different weight ratios, and the effect of the solution ratios was investigated. The morphologies and mechanical and swelling properties of the generated fibres were analysed. Indirect cytotoxicity studies indicated that the CS/PVA nanofibre mats were non-toxic to normal human fibroblast cells. The CS-HOBt/PVA and CS-EDTA/PVA nanofibre mats demonstrated satisfactory antibacterial activity against both gram-positive and gram-negative bacteria, and an in vivo wound healing test showed that the CS-EDTA/PVA nanofibre mats performed better than gauze in decreasing acute wound size during the first week after tissue damage. In conclusion, the biodegradable, biocompatible and antibacterial CS-EDTA/PVA nanofibre mats have potential for use as wound dressing materials.

A novel electrospun membrane based on moxifloxacin hydrochloride/poly(vinyl alcohol)/sodium alginate for antibacterial wound dressings in practical application

“…The amount of released MH was determined using a high-performance liquid chromatography (HPLC) (Kenawy et al, 2002;Charernsriwilaiwat et al, 2012) system equipped with a quaternary gradient pump unit, a variable wavelength detector, an auto-sampler and a thermostated column compartment. Sample analyses were performed on a Diamonsil C18 column (250 mm long  4.6 mm I.D.…”

“…The calculated average diameter of the healing residue area was used to determine the healing percentage (Yang et al, 2011;Charernsriwilaiwat et al, 2012) following equation:…”

“…PVA is a non-toxic, hydrophilic polymer with biodegradation and adhesive properties that can form solid hydrogen bonds with water (Bolto et al, 2009;Garg et al, 2014). Many researchers have utilized electrospinning to fabricate PVA nanofibrous scaffolds for use as wound healing scaffolds (Draize et al, 1944;Cencetti et al, 2012;Charernsriwilaiwat et al, 2012;Nitanan et al, 2013). A characteristic of materials designed for wound dressing is its antimicrobial effect.…”

“…To overcome this issue, modern dressings as vehicles for delivering therapeutic agents to wound sites have been applied in the form of films, sponges, foams, hydrogels (Cui et al, 2011;Balata et al, 2014;Vasile et al, 2014). And recently nanofibrous membranes (NFM) produced via electrospinning are beginning to emerge as wound dressing materials (Sill & von Recum, 2008;Charernsriwilaiwat et al, 2012;Ignatova et al, 2013;Mogosanu & Grumezescu, 2014), due to the unique properties of electrospun mats -high specific surface area and small-size pores are very favorable for the adsorption of body fluids and for preventing penetration of bacteria and thus provide good conditions for wound healing (Zahedi et al, 2010;Rieger et al, 2013).…”

Effect of Chitosan Gel on Wound Healing: Experimental Study in Donkeys

“…Chitosan promotes granulation, organization, and normal tissue regeneration, and help in recovering the original tensile strength of the wound (Okamoto et al, 1995;Ueno et al, 2001). Chitosan forms a semipermeable sheet upon the wound that maintains a sterile condition beneath a dry scab, therefore, preventing wound dehydration and contamination (Charernsriwilaiwat et al, 2014;Laura et al, 2014). Bioactive materials contain chitosan, was utilized recently as bone grafting substitutes due to its known osteoconductive and osteogenesis properties (Nandi et al, 2010;Emara et al, 2013).…”